Exploring the impact of Helicobacter pylori on gut microbiome composition

Abstract

Helicobacter pylori ( H . pylori ) is known to colonize gastric mucosa, induce inflammation, and alter gastric microbiota resulting in a spectrum of gastric diseases. Likewise, changes in gut microbiota have recently been linked with various metabolic and inflammatory diseases. While extensive number of studies were published examining the relationship between H . pylori and gastric microbiota, little is known about the impact of H . pylori on downstream gut microbiota. In this study, we performed 16 S rRNA and ITS2-based microbial profiling analysis of 60 stool samples from adult individuals. Remarkably, the gut microbiota of H . pylori infected individuals was shown to be increased of members belonging to Succinivibrio, Coriobacteriaceae, Enterococcaceae, and Rikenellaceae. Moreover, gut microbiota of H . pylori infected individuals was shown to have increased abundance of Candida glabrata and other unclassified Fungi. These results links possible role for H . pylori -associated changes in the gut microbiota in intestinal mucosal barrier disruption and early stage colorectal carcinoma deployment. Altogether, the identified differences in bacterial and fungal composition provides important information that may eventually lead to the development of novel biomarkers and more effective management strategies.

Lack of significant differences between gastrointestinal tract microbial population structure of Helicobacter pylori‐infected subjects before and 2 years after a single eradication event

“…Current evidence indicates that H pylori eradication therapy is associated with significant disturbances of the intestinal microbiota-a decrease in bacterial diversity, as well as alterations in the abundance of Bifidobacteria, Enterococci, Lactobacilli, Actinobacteria, butyrate producers, and detrimental bacteria, have been shown. [22][23][24][25][26] Additionally, one study reported that perturbations of the gut microbiota in some cases remain for up to 4 years after the treatment is completed. 27 While the emphasis for majority of H pylori eradication-gut microbiome studies is on short-term impact of antimicrobial therapy, the potential adverse effects to microbiome in a long-term period are insufficiently studied.…”

Gut Microbiota Changes During Helicobacter Pylori Eradication with Vonoprazan-Containing Triple Therapy Among Adolescents: A Prospective Multicenter Study in Japan

“…Several studies showed that H. pylori infection in children caused the changes in the gut microbiota. In adults with H. pylori infection, the intestinal microbiota has reduced diversity [22]. Benavides-Ward et al reported that children with H. pylori infection increased the number of bacteria, including Proteobacteria, Clostridium, Firmicutes, and Prevotella, in the gut microbiota [18].…”

Helicobacter pylori infection and eradication: Exploring their impacts on the gastrointestinal microbiota

“… 31,32 Strikingly, most studies investigating the relationship between H pylori infection and gut microbial diversity provided a positive correlation (Figure 2; Table 2). 31,33‐36 As higher microbial diversity is usually associated with an overall better health state, it will be interesting to explore whether the increased intestinal microbial diversity upon H pylori infection provides some beneficial effects to the host. Significant differences in microbial community structures were found between the H pylori ‐infected and negative control groups 33‐36 .…”

“… 31,33‐36 As higher microbial diversity is usually associated with an overall better health state, it will be interesting to explore whether the increased intestinal microbial diversity upon H pylori infection provides some beneficial effects to the host. Significant differences in microbial community structures were found between the H pylori ‐infected and negative control groups 33‐36 . The compositional analysis at the phylum level discovered that the abundance of Proteobacteria significantly increased in infected individuals, which could be due to the transfer of Helicobacter from the stomach to the intestinal lumen 6,34 .…”

“…Thus, functional shifts in microbiota were likely to be site‐specific as changes observed in the intestinal tract exhibited a different trend with those in the stomach 23,31,34 . Although the goal is to explore the impacts of H pylori infection on the intestinal flora, most recent studies performed sequencing analysis on fecal samples 31,33‐37,39 . Previous studies addressed that the profiles of fecal microbiota only partially recapitulated those of the mucosal microbiota 10,44 .…”