Exploring the genetics of lesion and nodal resistance in pea (Pisum sativum L.) to Sclerotinia sclerotiorum using genome‐wide association studies and RNA‐Seq

Abstract

The disease white mold caused by the fungus Sclerotinia sclerotiorum is a significant threat to pea production, and improved resistance to this disease is needed. Nodal resistance in plants is a phenomenon where a fungal infection is prevented from passing through a node, and the infection is limited to an internode region. Nodal resistance has been observed in some pathosystems such as the pea (Pisum sativum K E Y W O R D Sgenome-wide association study, glutathione S-transferase, lesion resistance, nodal resistance, pea (Pisum sativum L.), RNA-Seq, white mold (Sclerotinia sclerotiorum)

A Sclerotinia sclerotiorum Transcription Factor Involved in Sclerotial Development and Virulence on Pea

“…The susceptible line Lifter exhibited a larger stem lesion than the resistant line PI240515, and the Lifter plants showed lodging but not PI240515. At 48 hpi, the lesions observed on the stem and leaf were larger on Lifter than on PI240515; additional phenotype details can be found in a report by Chang et al (8). Two biologically replicated in planta and in vitro samples were harvested at 12, 24, and 48 hpi, and RNA was extracted and sequenced as described previously by Chang et al (8).…”

“…At 48 hpi, the lesions observed on the stem and leaf were larger on Lifter than on PI240515; additional phenotype details can be found in a report by Chang et al (8). Two biologically replicated in planta and in vitro samples were harvested at 12, 24, and 48 hpi, and RNA was extracted and sequenced as described previously by Chang et al (8). The raw RNA sequences were deposited in NCBI SRA database.…”

“…sclerotiorum. Transcriptomic analyses using RNA-seq have become a powerful approach to explore plant resistance to white mold (8, 9) and virulence mechanisms of S. sclerotiorum (10, 11).…”

“…Two pea lines, a partially resistant line (PI240515) and a susceptible line (Lifter), were used as host plants (8). S.…”

The complexity of the Sclerotinia sclerotiorum pathosystem in soybean: virulence factors, resistance mechanisms, and their exploitation to control Sclerotinia stem rot

“…Expression data from soybean and other crop plants will provide valuable information on soybean susceptibility factors for gene editing or post transcriptional gene silencing and gene candidates for cis or transgenic plant development. RNAseq methods were used to evaluate the interaction of S. sclerotiorum with crop hosts such as, canola (Joshi et al 2016;Girard et al 2017;Seifbarghi et al 2017), pea (Chang et al 2018), and common bean (Oliveira et al 2015).…”