Exploring the bioactive potential of Serriatia marcescens VITAPI (Acc: 1933637) isolated from soil

Muthukumar, Aruna; Pradeep, Pallavi; Thigale, Isha; Mohanasrinivasan, V.; Naine, S. Jemimah; Devi, C. Subathra

doi:10.1007/s11515-016-1430-2

Cited by 9 publications

(11 citation statements)

References 7 publications

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“…The potential antioxidant capacity of PG has been reported in some studies, such as Muthukumar et al [ 34 ], Arivizhivendhan et al [ 35 ], and Nguyen et al [ 11 ], who found the max activity of the DPPH radical scavenging capacity of PG to be 86%, 99%, and 98%, respectively. However, few data on the ABTS radical scavenging of PG have been reported so far [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…The result of the biological activities in this study confirmed that the purified PG produced by S. marcescens TNU02 PG was active molecule with potential anticancer properties. The potential antioxidant capacity of PG has been reported in some studies, such as Muthukumar et al [34], Arivizhivendhan et al [35], and Nguyen et al [11], who found the max activity of the DPPH radical scavenging capacity of PG to be 86%, 99%, and 98%, respectively. However, few data on the ABTS radical scavenging of PG have been reported so far [35].…”

Section: Antioxidant and Anticancers Activities Of Purified Pgmentioning

confidence: 87%

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Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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This study aimed to establish the culture process for the cost-effective production of prodigiosin (PG) from demineralized crab shell powder (de-CSP), a fishery processing byproduct created via fermentation. Among the tested PG-producing strains, Serratia marcescens TNU02 was demonstrated to be the most active strain. Various ratios of protein/de-CSP were used as the sources of C/N for PG biosynthesis. The PG yield was significantly enhanced when the casein/de-CSP ratio was controlled in the range of 3/7 to 4/6. TNU02 produced PG with a high yield (5100 mg/L) in a 15 L bioreactor system containing 4.5 L of a newly-designed liquid medium containing 1.6% C/N source (protein/de-CSP ratio of 3/7), 0.02% (NH4)2SO4, 0.1% K2HPO4, and an initial pH of 6.15, at 27 °C for 8 h in dark conditions. The red pigment was purified from the culture broth and then quantified as being PG by specific Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and UV spectra analysis. The purified PG demonstrated moderate antioxidant and effective inhibition against four cancerous cell lines. Notably, this study was the first to report on using crab wastes for PG bioproduction with high-level productivity (5100 mg/L) in a large scale (4.5 L per pilot) in a short period of fermentation time (8 h). The salt compositions, including (NH4)2SO4 and K2HPO4, were also a novel finding for the enhancement of PG yield by S. marcescens in this report.

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Section: Resultsmentioning

confidence: 99%

Section: Antioxidant and Anticancers Activities Of Purified Pgmentioning

confidence: 87%

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Nguyen

et al. 2020

Marine Drugs

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“…The antioxidant capacity of PG and α-tocopherol was also expressed as IC50 values of 79.1 and 38.5 µg/mL, respectively. PG has been reported to show great DPPH radical scavenging capacity of 86% and 99% [54,55], but with no reports on its IC50 value. However, there are rare reports of DPPH radical scavenging capacity of PG beyond the above-cited papers; as such, the result in this study contributes to reconfirm and show in detail the DPPH radical scavenging activity of PG, and also indicates that PG may be a good candidate as an anti-radical scavenging compound.…”

Section: Evaluation Of Biological Activities Of Pgmentioning

confidence: 99%

Microbial Reclamation of Chitin and Protein-Containing Marine By-Products for the Production of Prodigiosin and the Evaluation of Its Bioactivities

Nguyen

Wang

2020

Polymers

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Chitin and protein-containing marine by-products (CPCMBPs), including crab shells, squid pens, and shrimp shells, were investigated as the sole carbon/nitrogen (C/N) source for prodigiosin (PG) production by Serratia marcescens TNU01 in a 250 mL Erlenmeyer flask and a 10 L bioreactor system. Among the used C/N source of CPCMBPs, squid pens powder (SPP) showed the most optimum PG productivity. Different ratios of chitin/protein combination were also used as the C/N sources for PG production. With a similar chitin/protein ratio (4/6) of squid pens, a significant PG productivity was achieved when the chitin/protein ratios were controlled in the range of 3/7–4/6. Maximum PG yield (3450 mg/L) by S. marcescens TNU01 was achieved in the bioreactor system containing 3 L medium of 1.75% SPP, 0.03% K2HPO4, and 0.05% MgSO4 at 25 °C for 12 h in dark. The results of in vitro bioassays reveal that the purified PG possesses acetylcholinesterase inhibitory activity and antioxidant as well as anticancer activities. This study suggests that squid pens may have the potential to be used for cost effective production of bioactive PG at a large-scale.

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“…For comparison, mitomycin C, a commercial anti-cancer compound was also tested, and it showed weaker activity with IC 50 values of 0.11, 0.1, 0.14, and 0.15 µg/mL, respectively [ 59 ]. Muthukumar et al [ 145 ] studied the antioxidant, anti-inflammatory, and cytotoxic properties of PG produced by S. marcescens VITAPI and observed potent radical scavenging effect of the extracted PG at 86%, which was significant in comparison to ascorbic acid as a standard [ 145 ]. The in vitro anti-inflammatory effect of PG in controlled experimental conditions revealed its protection at 88% and inhibition in a concentration-dependent manner.…”

Section: Bioactivity and Application Of Pgmentioning

confidence: 99%

“…The in vitro anti-inflammatory effect of PG in controlled experimental conditions revealed its protection at 88% and inhibition in a concentration-dependent manner. The cytotoxic bioassay of PG showed the IC 50 value as 50 μg/mL at 63% cytotoxicity [ 145 ]. El-Bondkly et al [ 91 ] reported the cytotoxic activities of PG pigments produced by Streptomyces fusant NRCF69 against three human cancer cell lines, including colon cancer cell line (HCT-116), liver cancer cell line (HEPG-2), and breast cancer cell line (MCF-7) [ 91 ].…”

Section: Bioactivity and Application Of Pgmentioning

confidence: 99%

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

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Exploring the bioactive potential of Serriatia marcescens VITAPI (Acc: 1933637) isolated from soil

Cited by 9 publications

References 7 publications

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Utilization of Crab Waste for Cost-Effective Bioproduction of Prodigiosin

Microbial Reclamation of Chitin and Protein-Containing Marine By-Products for the Production of Prodigiosin and the Evaluation of Its Bioactivities

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

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