Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis

Yoo, Keunje; Yoo, Hyunji; Lee, Jangho; Choi, Eun Joo; Park, Joonhong

doi:10.1007/s12275-020-9309-y

Cited by 51 publications

(34 citation statements)

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“…Metagenomics rapid annotation subsystem technology (MG-RAST) was used to identify bacterial taxonomic classification [22], and a column chart comparing the relative abundance of each class was generated. All clean data were also searched for MGEs against an offline database, including integrons, gene cassettes, insertion sequences and plasmids, downloaded from the INTEGRALL and NCBI RefSeq databases [6,23]. If the nucleotide sequence identity of the best BLASTn hit was ≥90% over an alignment of ≥50 bp with an e-value ≤ 1 × 10 −5 , it was annotated as an integron, insertion sequence or gene cassette.…”

Section: Bioinformatics Analysismentioning

confidence: 99%

“…The removal of antibiotics is attributed to biodegradation and biosorption onto activated sludge [27][28][29]. In addition, the MLE anoxic/aerobic tanks in the activated sludge process may not provide good conditions for the proliferation of ARG-carrying bacteria; hence, the replication and dissemination of ARGs were not favored [6,30]. However, it is of concern that tetracycline and sulfonamide resistance genes were still high in the effluent because these resistance genes can persist under harsh environmental conditions, which may potentially transfer antibiotic resistance to bacteria via HGT (horizontal gene transfer) [21,31].…”

Section: Diversity and Occurrence Of Args From Metagenomic Analysismentioning

confidence: 99%

“…Aquatic environments are regarded as a major source for the aggregation and dissemination of antibiotic resistance genes (ARGs) because they are easily influenced by human activities [4,5]. In particular, the emergence of ARGs could significantly increase the spread of antibiotic resistance, especially via mobile genetic elements (MGEs) including plasmids, integrons and insertion sequences [4][5][6]. There is growing evidence that wastewater treatment plants (WWTPs) have received special attention in playing a major role in the proliferation of antibiotic resistance into other environmental systems, such as rivers, sediment, and oceans [6].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the emergence of ARGs could significantly increase the spread of antibiotic resistance, especially via mobile genetic elements (MGEs) including plasmids, integrons and insertion sequences [4][5][6]. There is growing evidence that wastewater treatment plants (WWTPs) have received special attention in playing a major role in the proliferation of antibiotic resistance into other environmental systems, such as rivers, sediment, and oceans [6]. Because WWTPs are mainly designed for the removal of physical and chemical pollutants, ARGs and antibiotic-resistant bacteria (ARB) are usually not considered for the effective removal of antibiotics in WWTPs.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have consistently reported relatively high levels of ARGs and MGEs and even heavy metal resistance genes in WWTPs and in each process in wastewater treatment systems [6,8,9]. However, most previous studies preliminarily focused on the identification of ARGs and ARB during the wastewater treatment process, such as activated sludge and anaerobic digestion processes, not considering the scale and treatment capacity of WWTPs [6,8].…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Yoo

Lee

2021

Antibiotics

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Although extensive efforts have been made to investigate the dynamics of the occurrence and abundance of antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs), understanding the acquisition of antibiotic resistance based on the WWTP scale and the potential effects on WWTPs is of relatively less interest. In this study, metagenomic analysis was carried out to investigate whether the WWTP scale could be affected by the prevalence and persistence of ARGs and mobile genetic elements (MGEs). As a result, 152 ARG subtypes were identified in small-scale WWTP samples, while 234 ARG subtypes were identified in large-scale WWTP samples. Among the detectable ARGs, multidrug, MLS (macrolide–lincosamide–streptogramin), sulfonamide, and tetracycline resistance genes had the highest abundance, and large and small WWTPs had similar composition characteristics of ARGs. In MGE analysis, plasmids and integrons were 1.5–2.0-fold more abundant in large-scale WWTPs than in small-scale WWTPs. The profile of bacteria at the phylum level showed that Proteobacteria and Actinobacteria were the most dominant bacteria, representing approximately 70% across large- and small-scale WWTPs. Overall, the results of this study elucidate the different abundances and dissemination of ARGs between large- and small-scale WWTPs, which facilitates the development of next-generation engineered wastewater treatment systems.

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Section: Bioinformatics Analysismentioning

confidence: 99%