Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Yoo, Keunje; Lee, Gihan

doi:10.3390/antibiotics10020188

Cited by 16 publications

(4 citation statements)

References 63 publications

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“…In this study, one Arr, eleven Erm and two Sul proteins were predicted to have undergone recombination. The arr, erm and sul genes are usually associated with mobile genetic elements such as plasmids, integrons and transposons, which facilitate the dissemination of antibiotic resistance through HGT [51][52][53]. Our results are consistent with those in earlier studies [54,55] that reported Erm proteins to be frequently involved in HGT in grampositive bacteria.…”

Section: Discussionsupporting

confidence: 92%

The resistomes of Mycobacteroides abscessus complex and their possible acquisition from horizontal gene transfer

2022

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Background Mycobacteroides abscessus complex (MABC), an emerging pathogen, causes human infections resistant to multiple antibiotics. In this study, the genome data of 1,581 MABC strains were downloaded from NCBI database for phylogenetic relatedness inference, resistance profile identification and the estimation of evolutionary pressure on resistance genes in silico. Results From genes associated with resistance to 28 antibiotic classes, 395 putative proteins (ARPs) were identified, based on the information in two antibiotic resistance databases (CARD and ARG-ANNOT). The ARPs most frequently identified in MABC were those associated with resistance to multiple antibiotic classes, beta-lactams and aminoglycosides. After excluding ARPs that had undergone recombination, two ARPs were predicted to be under diversifying selection and 202 under purifying selection. This wide occurrence of purifying selection suggested that the diversity of commonly shared ARPs in MABC have been reduced to achieve stability. The unequal distribution of ARPs in members of the MABC could be due to horizontal gene transfer or ARPs pseudogenization events. Most (81.5%) of the ARPs were observed in the accessory genome and 72.2% ARPs were highly homologous to proteins associated with mobile genetic elements such as plasmids, prophages and viruses. On the other hand, with TBLASTN search, only 18 of the ARPs were identified as pseudogenes. Conclusion Altogether, our results suggested an important role of horizontal gene transfer in shaping the resistome of MABC.

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Section: Discussionsupporting

confidence: 92%

The resistomes of Mycobacteroides abscessus complex and their possible acquisition from horizontal gene transfer

2022

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“…The high prevalence of the tetracycline resistance genes (tetQ, tetW, tetM, and tetO) might be the result of antibiotic selective pressure, considering that tetracyclines persist for a long time in the environment and are used frequently in human and veterinary medicine and aquacultural and agricultural practices [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries

et al. 2022

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Anthropogenic pressure is known to be a key driver of antimicrobial resistance (AMR) dissemination in the environment. Especially in lower income countries, with poor infrastructure, the level of AMR dissemination is high. Therefore, we assessed the levels and diversity of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in Lebanese rivers at estuaries’ sites (n = 72) of the Mediterranean Sea in spring 2017 and winter 2018. Methods: A combined approach using culture techniques and high throughput qPCR were applied to identify ARB and ARGs in rivers along the Lebanese coast. Results: Multidrug-resistant Gram-negative (Enterobacterales and Pseudomonas spp.) and Gram-positive bacterial pathogens were isolated. Levels of ARGs were highest in the winter campaign and areas with high anthropogenic activities and population growth with an influx of refugees. Conclusion: Qualitative analysis of ARB and the analysis of the Lebanese estuaries’ resistome revealed critical levels of contamination with pathogenic bacteria and provided significant information about the spread of ARGs in anthropogenically impacted estuaries.

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“…Proteobacteria, Chloroflexi, and Acidobacteria were the most abundant under phyla level in the estuaries [10]. Proteobacteria were also the most dominant bacterial phylum in wastewater treatment plants (WWTPs), Tai Lake and Qinghai Lake [13,15,33]. It suggested that Proteobacteria and Actinobacteria appeared to be the main microbial colonizers of natural substrates in aquatic environments [34].…”

Section: Microbial Community Structurementioning

confidence: 98%

“…3B). In previous studies, multidrug was most frequent in urban environments [20], the drinking water supply system [36], estuaries [37], urban-lake drinking water [38], as well as WWTPs [33]. The highest content in the sediment of Tai Lake was the multidrug, followed by macrolidelincosamide-streptogramin (MLS), and bacitracin [15].…”

Section: Args Compositionmentioning

confidence: 99%

Metagenomic Insight into the Prevalence and Distribution of Antibiotic Resistance Genes in China’s Largest Freshwater Lake

Liu,

Yu,

Jian

et al. 2024

Pol. J. Environ. Stud.

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Lakes offer a primary setting for the collection and spread of antibiotic resistance genes (ARGs) within natural habitats, so it is of great importance and urgency to investigate the characteristics of ARGs in such aquatic ecosystems. Herein, the occurrence and distribution of microbial populations, ARGs, and mobile genetic elements (MGEs) in Poyang Lake, the largest freshwater lake in China, were analyzed in-depth through metagenomic sequencing. Surface water and sediment samples were examined simultaneously at 10 sampling sites covering the inlets of five tributaries and central lake areas. Results showed that the antibiotic concentration was at a low level in both surface water and sediment. Proteobacteria and Actinobacteria were the two most prevalent phyla across all sampling sites. The surface water displayed a greater relative abundance of ARGs than the sediment. Multidrug resistance genes were the most abundant and diverse ARGs. Gene uppP was the most abundant ARG in both the surface water and sediment samples. ARGs in the sediment showed a decreasing trend from inlet to outlet of Poyang Lake, while in the water, the relative abundance of ARGs increased first and then decreased from inlet to outlet. ARG levels in Poyang Lake are thought to be closely related to human activity. A broad spectrum of MGEs series was recognized in both surface water and sediment samples; the abundance of MGEs was consistent with ARGs' abundance. Additionally, the makeup of the microbial community significantly influenced the resistome, suggesting that the composition of ARGs was largely affected by the microbial community. These findings indicate that lake water can act as a significant medium for ARGs dispersion, and the clustering and spread of ARGs are prone to the effects of human activities in aquatic habitats.

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Investigation of the Prevalence of Antibiotic Resistance Genes According to the Wastewater Treatment Scale Using Metagenomic Analysis

Cited by 16 publications

References 63 publications

The resistomes of Mycobacteroides abscessus complex and their possible acquisition from horizontal gene transfer

The resistomes of Mycobacteroides abscessus complex and their possible acquisition from horizontal gene transfer

Resistome Diversity and Dissemination of WHO Priority Antibiotic Resistant Pathogens in Lebanese Estuaries

Metagenomic Insight into the Prevalence and Distribution of Antibiotic Resistance Genes in China’s Largest Freshwater Lake

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