Exploring different photodynamic therapy parameters to optimize elimination of Enterococcus faecalis in planktonic form

Soares, Janir Alves; Soares, Suelleng Maria Cunha Santos; Tavarez, Rudys Rodolfo de Jesus; Rizzi, Cláudia de Castro; Rodrigues, Silvana Cristina Gama Vaz; Filho, Etevaldo Matos Maia; Brito-Júnior, Manoel; Pereira, Rodrigo Dantas; Magalhães, Paula Prazeres; Farias, Luiz de Mácêdo

doi:10.1016/j.pdpdt.2018.03.009

Cited by 16 publications

(18 citation statements)

References 31 publications

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“…They found PDT very effective against bacteria. 27 Similar to the results of our study, the results of a study by Hoedke et al revealed that the application of NaOCl with PDT improved its antibacterial function. 28 Souzo et al observed that NaOCl had a significantly better antibacterial function than PDT.…”

Section: Discussionsupporting

confidence: 92%

Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

et al. 2020

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Introduction: Enterococcus faecalis is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating E. faecalis from infected root canals. Methods: Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. Results: The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% (P=0.011) and when the system was used with sodium hypochlorite, it was 100% (P=0.001). The antibacterial effect of PDT was 90.08% (P=0.011) and it was 99.7% when PDT was combined with sodium hypochlorite (P=0.011). Conclusion: All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.

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Section: Discussionsupporting

confidence: 92%

Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

et al. 2020

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“…The number of bacteria was adjusted using a standard curve. For the erythrosine supplement group, 100 μL of erythrosine was added, whereas 100 μL of PBS was added to the control groups [14,15].…”

Section: Bacterial Strainmentioning

confidence: 99%

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Park¹,

Park²,

Lee³

et al. 2020

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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The present study is aimed to assess the effect of antimicrobial photodynamic therapy (aPDT) on Streptococcus mutans biofilm through teeth whitening light emitting diode (LED).Planktonic and dynamic biofilm state cultures of S. mutans were used. Erythrosine 20 μM/L was used as the photosensitizer. Irradiation was performed by exposing cultures to clinic and homecare whitening LEDs for 15 minutes. The viability was measured through Colony Forming Unit counts and confocal laser scanning microscopy.aPDT using whitening LEDs and erythrosine significantly decreased the CFU count of S. mutans compared to that in the control group. Dynamic biofilm group showed more resistant features to aPDT compared with planktonic state. Clinic and homecare whitening LED device showed similar antimicrobial effect. The whitening LED, which could irradiate the entire oral arch, showed a significant photodynamic effect on cariogenic S. mutans biofilm. aPDT mediated by erythrosine and LEDs used for teeth whitening exhibited promising antimicrobial activity. Abstract 70Corresponding author : Juhyun Lee

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“…Each root was autoclaved and the apical foramen was sealed with a cyanoacrylate in a laminar flow chamber (Marconi Equipment, Piracicaba, São Paulo, Brazil). The root canals were inoculated with a fresh suspension of E. faecalis (ATCC 19433) at 10 9 CFU/mL in BHI, followed by incubation at 37°C, according to Soares et al [24]. Every 48 hours, the root canals were reinoculated with fresh suspension until a final period of 3 weeks.…”

Section: Methodsmentioning

confidence: 99%

Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen

Soares

Carvalho

Soares

et al. 2019

The Scientific World Journal

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Objective To quantify Enterococcus faecalis density in root canal dentin after chemomechanical preparation (CMP) using alternated irrigating regimen. Methodology Root canals (RC) were contaminated with E. faecalis (ATCC 19433) for 3 weeks and evident biofilms were obtained. After initial sampling (S1), the CMP was aided by irrigants: saline solution (control; n=12), a conventional regimen (CR) (group 1; n=12) using 5.25% NaOCl and a final rinse with 17% EDTA, and an alternating regimen (AR) of intercalated use of NaOCl and EDTA (group 2, n=12), followed by a second sampling (S2). After 2 weeks, S3 was obtained. Two roots were analyzed by scanning electron microscopy. Each root was divided into cervical, mild, and apical segments and sampling of the superficial (n=90) and deep (n=90) dentin layers was obtained using Gates-Glidden burs. The E. faecalis density (CFU/mg) in log10 was categorized as residual (0 > 0.2), moderate (0.2 ≥ 0.5), or elevated (> 0.5). The prevalence of positive samples in BHI and BHI-A was analyzed by Pearson's chi-square test. The data were normalized by a log10 transformation of CFU and were analyzed by one-way ANOVA and Tukey's tests. Results Biofilms were observed only in the control root canal walls. Topographically, the controls and CR showed similar distributions of E. faecalis in the dentin. Microbiologically positive root canals harbored much E. faecalis in the adjacent dentin (p < 0.05). Irrigating saline provided moderate density of E. faecalis in the dentin while CR and AR resulted in a residual density of microorganisms (p < 0.05). Conclusions The Enterococcus faecalis density in dentin was influenced by the irrigating regimen and the microbiological status of the root canal. The CMP aided by the alternating regimen interfered with the recolonization of the root canal and topographic distribution of Enterococcus in root dentin.

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Exploring different photodynamic therapy parameters to optimize elimination of Enterococcus faecalis in planktonic form

Cited by 16 publications

References 31 publications

Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

Application of Teeth Whitening LED for Prevention of Dental Caries : Antimicrobial Photodynamic Therapy Approach

Pattern of Disinfection of Root Canal Dentin by Alternated Acid-Base Irrigating Regimen

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