Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells

Pan, Tai‐Long; Wang, Pei‐Wen

doi:10.1155/2012/734987

Cited by 28 publications

(35 citation statements)

References 42 publications

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“…HepG2 cells were plated onto 24‐well plates (2 × 10 4 cells/well), maintained in antibiotic‐free medium for 6 h, and transfected with a mixture containing Opti‐MEM, 8 μL/well Lipofectamine 2000 (Invitrogen, San Diego, CA, USA) and either 0.5 μg/well scramble siRNA or a mixture of three VCP siRNA (siVCP, smart pool; Invitrogen). At 24 h posttransfection, cells were pretreated with 100 μg/mL SEE followed by 1 mM H 2 O 2 dissolved in medium for another 24 h. Then, the cells were harvested for MTT assays . Cell viability ratio =

[({OD}_{control}) - ({OD}_{sample})] / [({OD}_{control}) - ({OD}_{H_{2} O_{2}})]

.…”

Section: Methodsmentioning

confidence: 99%

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Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia‐reperfusion injury

Pan

Wang

et al. 2013

Proteomics

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Hepatic ischemia-reperfusion (IR) injury is a common clinical problem and ROS may be a contributing factor on IR injury. The current study evaluates the potential protective effect of saffron ethanol extract (SEE) in a rat model upon hepatic IR injury. Caspases 3 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) results showed increased cell death in the IR samples; reversely, minor apoptosis was detected in the SEE/IR group. Pretreatment with SEE significantly restored the content of antioxidant enzymes (SOD1 and catalase) and remarkably inhibited the intracellular ROS concentration in terms of reducing p47phox translocation. Proteome tools revealed that 20 proteins were significantly modulated in protein intensity between IR and SEE/IR groups. Particularly, SEE administration could attenuate the carbonylation level of several chaperone proteins. Network analysis suggested that saffron extract could alleviate IR-induced ER stress and protein ubiquitination, which finally lead to cell apoptosis. Taken together, SEE could reduce hepatic IR injury through modulating protein oxidation and our results might help to develop novel therapeutic strategies against ROS-caused diseases.

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[({OD}_{control}) - ({OD}_{sample})] / [({OD}_{control}) - ({OD}_{H_{2} O_{2}})]

.…”

Section: Methodsmentioning

confidence: 99%

“…Cell viability ratio =

[({OD}_{control}) - ({OD}_{sample})] / [({OD}_{control}) - ({OD}_{H_{2} O_{2}})]

. Determination of cell cycle by flow cytometry was described previously . Results shown are the representative of three separate experiments.…”

Section: Methodsmentioning

confidence: 99%

Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia‐reperfusion injury

Pan

Wang

et al. 2013

Proteomics

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“…The result was detected by using ECL reagent. The density of Western blot bands was quantified by GeneTools software as previous reported .…”

Section: Methodsmentioning

confidence: 99%

“…Cells were then incubated with 7.5 μM Tan IIA for 6 h. Carboxy‐H2DCFDA (2 μM) was added to the wells and incubated for 30 min at 37°C. The cells were observed and photographed using a fluorescent microscope (Olympus microscope IX71) under DP72 PhotoImage system .…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis reveals tanshinone IIA enhances apoptosis of advanced cervix carcinoma CaSki cells through mitochondria intrinsic and endoplasmic reticulum stress pathways

et al. 2013

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Cervix cancer is the second most common cancer among women worldwide, whereas paclitaxel, the first line chemotherapeutic drug used to treat cervical cancer, shows low chemosensitivity on the advanced cervical cancer cell line. Tanshinone IIA (Tan IIA) exhibited strong growth inhibitory effect on CaSki cells (IC50 = 5.51 μM) through promoting caspase cascades with concomitant upregulating the phosphorylation of p38 and JNK signaling. Comprehensive proteomics revealed the global protein changes and the network analysis implied that Tan IIA treatment would activate ER stress pathways that finally lead to apoptotic cell death. Moreover, ER stress inhibitor could alleviate Tan IIA caused cell growth inhibition and ameliorate C/EBP-homologous protein as well as apoptosis signal-regulating kinase 1 mediated cell death. The therapeutic interventions targeting the mitochondrial-related apoptosis and ER stress responses might be promising strategies to conquer paclitaxel resistance.

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“…Recent studies have found that PHB is essential in the activation of both PI3 K/Akt and Raf/ERK signaling cascades . PHB interacts with and is phosphorylated at threonine 258 by Akt, which was suggested to be critical for the activation of the Akt signaling cascade .…”

Section: Introductionmentioning

confidence: 99%

The role of lipid raft translocation of prohibitin in regulation of Akt and Raf‐protected apoptosis of HaCaT cells upon ultraviolet B irradiation

2017

Molecular Carcinogenesis

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Prohibitin (PHB) plays a role in regulation of ultraviolet B light (UVB)-induced apoptosis of human keratinocytes, HaCaT cells. The regulatory function of PHB appears to be associated with its lipid raft translocation. However, the detailed mechanism for PHB-mediated apoptosis of these keratinocytes upon UVB irradiation is not clear. In this report, we determined the role of lipid raft translocation of PHB in regulation of UVB-induced apoptosis. Our data show that upon UVB irradiation PHB is translocated from the non-raft membrane to the lipid rafts, which is correlated with a release of both Akt and Raf from membrane. Overexpression of Akt and/or Raf impedes UVB-induced lipid raft translocation of PHB. Immunoprecipitation analysis indicates that UVB alters the interactions among PHB, Akt, and Raf. Reduced expression of PHB leads to a decreased phosphorylation of Akt and ERK, as well as a decreased activity of Akt, and increased apoptosis of the cells upon UVB irradiation. These results suggest that PHB regulates UVB-induced apoptosis of keratinocytes via a mechanism that involves detachment from Akt and Raf on the plasma membrane, and sequential lipid raft translocation.

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Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells

Cited by 28 publications

References 42 publications

Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia‐reperfusion injury

Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia‐reperfusion injury

Proteomic analysis reveals tanshinone IIA enhances apoptosis of advanced cervix carcinoma CaSki cells through mitochondria intrinsic and endoplasmic reticulum stress pathways

The role of lipid raft translocation of prohibitin in regulation of Akt and Raf‐protected apoptosis of HaCaT cells upon ultraviolet B irradiation

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