Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia‐reperfusion injury

Abstract

Hepatic ischemia-reperfusion (IR) injury is a common clinical problem and ROS may be a contributing factor on IR injury. The current study evaluates the potential protective effect of saffron ethanol extract (SEE) in a rat model upon hepatic IR injury. Caspases 3 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) results showed increased cell death in the IR samples; reversely, minor apoptosis was detected in the SEE/IR group. Pretreatment with SEE significantly restored the content of antioxidant enzymes (SOD1 and catalase) and remarkably inhibited the intracellular ROS concentration in terms of reducing p47phox translocation. Proteome tools revealed that 20 proteins were significantly modulated in protein intensity between IR and SEE/IR groups. Particularly, SEE administration could attenuate the carbonylation level of several chaperone proteins. Network analysis suggested that saffron extract could alleviate IR-induced ER stress and protein ubiquitination, which finally lead to cell apoptosis. Taken together, SEE could reduce hepatic IR injury through modulating protein oxidation and our results might help to develop novel therapeutic strategies against ROS-caused diseases.

Detoxification capacity and protective effects of medicinal plants (part 2): plant based review

“…Proteome tools revealed that 20 proteins were significantly modulated in protein intensity between IR and SEE/IR groups. Particularly, SEE administration attenuate the carbonylation level of several chaperone proteins [94].…”

Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach

“…As indicated in the proteome and analysis network results, upregulation of VCP as well as carbonylated proteins was shown in lead‐treated samples but not in the controls. This reflects the effort of hepatocytes in removing ER stress and attenuating the following ubiquitin–proteasome pathway initiated by lead administration .…”

“…As indicated in the proteome and analysis network results, upregulation of VCP as well as carbonylated proteins was shown in lead-treated samples but not in the controls. This reflects the effort of hepatocytes in removing ER stress and attenuating the following ubiquitin-proteasome pathway initiated by lead administration [18]. METK1 catalyzes the formation of S-adenosylmethionine, which serves as an oxidizing agent and a universal methyl donor in DNA repair and the biosynthesis of vitamins [29].…”

Integrated Proteome and Cytokine Profiles Reveal Ceruloplasmin Eliciting Liver Allograft Tolerance via Antioxidant Cascades

“…The numbers of stained and unstained cells were then counted from randomly chosen fields per slide within a high-power field (×200 magnifications) under an Olympus BX50 microscope. Image-Pro plus 4.5 (Media Cybernetics) image analysis software was used to quantify image signals ( 27 ).…”