Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE

Liu, Hongwei; Zhang, Jian; Zhou, Pingping; Sun, Hao; Katsarou, Martha Spyridoula; Drakoulis, Nikolaos

doi:10.3892/etm.2019.8009

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…[ 57 ] Overactive immune system following COVID-19 vaccination and increased specific auto-antibodies and certain factors in the serum might have led to this injury. [ 58 ]…”

Section: Discussionmentioning

confidence: 99%

Ocular adverse effects of COVID-19 vaccines: A systematic review

Kumari¹,

Anand²,

Sambyal

et al. 2022

J Family Med Prim Care

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The COVID-19 pandemic has led to the development and rollout of several vaccines worldwide at unprecedented pace. This systematic review of published literature has been undertaken to spread awareness among general physicians and ophthalmologists about the various reported adverse effects in the eye following COVID-19 vaccination. A systematic search was performed on 25 January 2022 through PuBMed, Medline and Google scholar for publications on ocular adverse effects after COVID-19 vaccination. One brief communication, four retrospective case series, sixteen case reports, and five letters to editors were included. Ocular manifestations most commonly appear in the uvea and retina. Other manifestations are seen on the eyelid, cornea and ocular surface, and in cranial nerves innervating the eye. The incidence rate of these manifestations is quite low after COVID-19 vaccinations. Our systematic review meticulously enumerates various adverse effects of COVID -19 vaccine on the eye. Most of these adverse effects are transient and observed to resolve without any sequelae except for cases of retinal and ophthalmic vascular occlusions and corneal graft rejections. An emphasis on close follow-up and a need to delay vaccination and modified therapy to control flare up of signs and symptoms in certain sub-populations, Graves’ disease (autoimmune etiology), pre-existing uveal inflammation and corneal graft cases are warranted. We need long-term, larger, multicentric studies to substantiate our findings and establish the causal relationship with certainty. Mass vaccinations to curb this pandemic after outweighing the ocular risks associated with it is warranted.

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“…[ 57 ] Overactive immune system following COVID-19 vaccination and increased specific auto-antibodies and certain factors in the serum might have led to this injury. [ 58 ]…”

Section: Discussionmentioning

confidence: 99%

Ocular adverse effects of COVID-19 vaccines: A systematic review

Kumari¹,

Anand²,

Sambyal

et al. 2022

J Family Med Prim Care

View full text Add to dashboard Cite

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“…IGHG3, which is also involved in acute rejection [27], may contribute to platelet activation during ischemic stroke, and it is expected to be a potential target for the treatment of the early phase of stroke [28]. Studies from other groups have found that IGHM is abnormally expressed in patients with conjunctivitis-related systemic lupus erythematosus and cholangiocarcinoma [29,30]. In our study, several of the proteins involved in immune regulation were significantly reduced in plasma, indicating considerable immunosuppression in patients with SAP and suggesting that these proteins may be potential biomarkers for SAP.…”

Section: Discussionmentioning

confidence: 99%

DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

Zhou

et al. 2022

Molecules

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Acute pancreatitis (AP) is a pancreatic inflammatory disease that varies greatly in course and severity. To further the understanding of the pathology of AP, we carried out data-independent acquisition-based proteomic analyses using proteins extracted from the plasma of patients with severe acute pancreatitis (SAP) (experimental group) and healthy volunteers (control group). Compared to the control group, there were 35 differentially expressed proteins (DEPs) in the plasma of patients with SAP. Of those, the expression levels for 6 proteins were significantly increased, and 29 proteins were significantly decreased. Moreover, six candidate biomarkers—VWF, ORM2, CD5L, CAT, IGLV3-10, and LTF—were matched as candidate biomarkers of the disease severity of AP. The area under the receiver operating characteristic of 0.903 (95% CI: 0.839, 0.967) indicated that this combination of these six candidate biomarkers had a good prediction accuracy for predicting the severity of AP. Our study provides specific DEPs that may be useful in the diagnosis and prognosis of SAP, which suggests new theoretical bases for the occurrence and development of SAP and offers potential novel treatment strategies for SAP.

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“…19,20 2D-DIGE allows covalent labeling of proteins in various samples (e.g., a control and a test group) with different fluorescent dyes, followed by mixing and simultaneous separation by two-dimensional gel electrophoresis (2D-GE) in a single gel. 21,22 Common dyes used are cyan fluorescent dyes, binding to the epsilon amino-group of lysine residues and infrared (IR) maleimide dyes, binding to free thiolgroups of cysteines. 23,24 For detection, the distinct fluorescence of the dyes is recorded, which intensity depends on the amount of the protein-attached dye.…”

Section: Introductionmentioning

confidence: 99%

“…For the investigation of proteome variations, the technique of fluorescent two‐dimensional difference gel electrophoresis (2D‐DIGE) presented for the first time by Ünlü et al has been established 19,20 . 2D‐DIGE allows covalent labeling of proteins in various samples (e.g., a control and a test group) with different fluorescent dyes, followed by mixing and simultaneous separation by two‐dimensional gel electrophoresis (2D‐GE) in a single gel 21,22 . Common dyes used are cyan fluorescent dyes, binding to the epsilon amino‐group of lysine residues and infrared (IR) maleimide dyes, binding to free thiol‐groups of cysteines 23,24 .…”

Section: Introductionmentioning

confidence: 99%

Transthyretin as a target of alkylation and a potential biomarker for sulfur mustard poisoning: Electrophoretic and mass spectrometric identification and characterization

Schmeißer¹,

Lüling

Steinritz

et al. 2021

Drug Testing and Analysis

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For the verification of exposure to the banned blister agent sulfur mustard (SM) and the better understanding of its pathophysiology, protein adducts formed with endogenous proteins represent an important field of toxicological research. SM and its analogue 2-chloroethyl ethyl sulfide (CEES) are well known to alkylate nucleophilic amino acid side chains, for example, free-thiol groups of cysteine residues. The specific two-dimensional thiol difference gel electrophoresis (2D-thiol-DIGE) technique making use of maleimide dyes allows the staining of free cysteine residues in proteins. As a consequence of alkylation by, for example, SM or CEES, this staining intensity is reduced. 2D-thiol-DIGE analysis of human plasma incubated with CEES and subsequent matrix-assisted laser desorption/ionization time-of-flight (tandem) mass-spectrometry, MALDI-TOF MS(/MS), revealed transthyretin (TTR) as a target of alkylating agents. TTR was extracted from SM-treated plasma by immunomagnetic separation (IMS) and analyzed after tryptic cleavage by microbore liquid chromatography-electrospray ionization high-resolution tandem-mass spectrometry (μLC-ESI MS/HR MS). It was found that the Cys 10 -residue of TTR present in the hexapeptide C(-HETE)PLMVK was alkylated by the hydroxyethylthioethyl (HETE)moiety, which is characteristic for SM exposure. It was shown that alkylated TTR is stable in plasma in vitro at 37 C for at least 14 days. In addition, C(-HETE)PLMVK can be selectively detected, is stable in the autosampler over 24 h, and shows linearity in a broad concentration range from 15.63 μM to 2 mM SM in plasma in vitro. Accordingly, TTR might represent a complementary protein marker molecule for the verification of SM exposure.

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Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE

Cited by 9 publications

References 23 publications

Ocular adverse effects of COVID-19 vaccines: A systematic review

Ocular adverse effects of COVID-19 vaccines: A systematic review

DIA-Based Proteomic Analysis of Plasma Protein Profiles in Patients with Severe Acute Pancreatitis

Transthyretin as a target of alkylation and a potential biomarker for sulfur mustard poisoning: Electrophoretic and mass spectrometric identification and characterization

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