Exploration of the role of the subodontoblastic layer in odontoblast-like cell differentiation after tooth drilling using Nestin-enhanced green fluorescent protein transgenic mice

Imai, Chihiro; Sano, Hiroto; Quispe-Salcedo, Ángela; Saito, Kotaro; Nakatomi, Mitsushiro; Ida‐Yonemochi, Hiroko; Ohshima, Hayato

doi:10.1016/j.job.2022.01.001

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…In dental pulp, it seems that the odontoblastic and subodontoblastic layers are a source for MSCs. This finding is in line with the high remodeling rate inside this area (44). their affiliated organizations, or those of the publisher, the editors and the reviewers.…”

Section: Limitations Of the Studysupporting

confidence: 83%

Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament

Heilen

Roßgardt²,

Dern-Wieloch³

et al. 2023

Front. Vet. Sci.

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IntroductionThe lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.MethodsIn this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure via immunohistochemistry of histological sections. For these analyses, several surface markers were applied.ResultsThe described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer.DiscussionOur findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies.

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Section: Limitations Of the Studysupporting

confidence: 83%