Exploration of Luteinizing hormone in murrah buffalo ( Bubalus bubalis ) urine: Extended surge window opens door for estrus prediction

Selvam, Ramu Muthu; Onteru, Suneel Kumar; Nayan, Varij; Sivakumar, M.; Singh, Dheer; Archunan, ‪Govindaraju

doi:10.1016/j.ygcen.2016.12.002

Cited by 12 publications

(17 citation statements)

References 46 publications

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“…The patterns of urinary LH forms are likely to be biologically meaningful, and proportional to pituitary production of the intact LH (Brindle et al., ), and a definite proof for the presence of circulating form of LH (Cole, ). Our preliminary experiments evidenced the compatibility of bovine serum LH kit for buffalo urinary LH detection (Selvam et al., ). Variation in the period of the onset of oestrus, observed from late evening of day −1 to early morning of day 0, and the end of oestrus, recorded from the early evening to late night of day 0, might be due to interindividual variation.…”

Section: Discussionmentioning

confidence: 56%

“…Several parameters including visual, behavioural and biochemical analysis were observed/performed regularly to detect the oestrus phase of individual animals. The days of the oestrous cycle were assigned as pre-oestrus (days 4-2), oestrus (day 0), ovulation (days 1-2) and post-oestrus (days 6-9), according to Rajanarayanan and Archunan (2004) with slight modification (Selvam et al, 2016).…”

Section: Screening Criteriamentioning

confidence: 99%

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Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed‐artificial insemination in murrah buffalo (Bubalus bubalis)

Selvam

Singh

et al. 2017

Reprod Domestic Animals

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Assessment of urine levels of luteinizing hormone (LH) for predicting the reproductive status of animals is in practice. The aim of this study was to predict the period of ovulation based on the urine levels of LH for timed-artificial insemination to increase the conception rate in buffaloes, which are naturally silent-oestrous animals. Level of LH in urine was assessed using ELISA, and a cut-off LH concentration for prediction of ovulation period was obtained using receiver operating characteristic analysis. Artificial insemination was performed before- and after -positive prediction of ovulation period adopting this method, and the rates of conception were assessed. Urine LH level of 105 mIU/ml (n = 14) was derived as a cut-off concentration which predicts the ovulation period. The buffaloes in the positively predicted group (day 1 or 2) inseminated via intracervical route had an increase in the conception rate (83.33%); however, the insemination in the before-positive-prediction group resulted in poor conception rates (day 0; 16.66%) compared to that of the naturally inseminated group (day 0; 75.0%). In conclusion, the urinary LH would possibly be a fairly reliable predictor of the ovulation period. The day when cut-off LH concentration is obtained may be taken as the most favourable time for artificial insemination, so as to attain a much better rate of conception in the buffalo.

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Section: Discussionmentioning

confidence: 56%

Section: Screening Criteriamentioning

confidence: 99%

Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed‐artificial insemination in murrah buffalo (Bubalus bubalis)

Selvam

Singh

et al. 2017

Reprod Domestic Animals

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“…The pattern of LH surge in buffalo urine was analysed and found that the extended LH surge window increases the success rate of prediction of ovulation period in this species. 4 In addition, an increased rate of conception was observed when inseminated artificially after positive-prediction of ovulation period through a cut-off LH value.5 Though, efficacy of LH peptide based prediction method was evidenced, sensitivity of this method was limited by occurrence of multiple LH waves with different amplitudes, before and after the core pre-ovulatory LH surge. …”

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confidence: 99%

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Urinary Peptides for Predicting Ovulation Period in Murrah Buffaloes: An Opinion

Archunan¹

2016

MOJPB

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was observed by a cost-effective, comprehensible estrus prediction model, 1 but this might better assist a valid biochemical parameter to increase the sensitivity of ovulation prediction. Though estrus-specific pheromones and proteins have been reported in buffaloes, 2,3 no sensitive biochemical kit has yet been developed for estrus detection and/or ovulation period prediction.Excretory fluids like urine reflect the internal status of the animal and have been proposed to be a better medium for assessing the reproductive status, non-invasively. Timed-artificial insemination by assessing urine for peptide hormones is in practice for many mammals. Luteinizing hormone (LH) is a heterodimeric glycoprotein, has an important role in ovulation, and its presence in urine is detected by its metabolite LHβ-core fragment (LHβcf). The pattern of LH surge in buffalo urine was analysed and found that the extended LH surge window increases the success rate of prediction of ovulation period in this species. 4 In addition, an increased rate of conception was observed when inseminated artificially after positive-prediction of ovulation period through a cut-off LH value.5 Though, efficacy of LH peptide based prediction method was evidenced, sensitivity of this method was limited by occurrence of multiple LH waves with different amplitudes, before and after the core pre-ovulatory LH surge. 4Increasing the sensitivity of the prediction method is indispensable, before the LH peptide is put forth for a portable kit development. In order to increase the sensitivity, certain valuable peptide markers in the same sample have to be identified and utilised. These markers should distinguish the period of ovulation from other phases of estrous cycle such as pre-estrus, estrus and post-estrus phases (Figure 1), where non-ovulatory LH surge and false prediction happens. Methods has to be put-forth for the effective extraction of proteins from buffalo urine and phase specific urinary proteins has to be documented through 2D Polyacrylamide gel followed by LC-MS/MS analysis. Ovulation prediction strip comprising antibodies for LH and phase specific proteins (separately) increases the sensitivity and reliability of the prediction of ovulation period, thereby achieves increased conception rate by determining precise time for artificial insemination.

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Detection of estrous biomarkers in the body exudates of Kangayam cattle (Bos indicus) from interplay of hormones and behavioral expressions

Ramachandran

Vinothkumar

Sankarganesh

et al. 2020

Domestic Animal Endocrinology

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Exploration of Luteinizing hormone in murrah buffalo ( Bubalus bubalis ) urine: Extended surge window opens door for estrus prediction

Cited by 12 publications

References 46 publications

Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed‐artificial insemination in murrah buffalo (Bubalus bubalis)

Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed‐artificial insemination in murrah buffalo (Bubalus bubalis)

Urinary Peptides for Predicting Ovulation Period in Murrah Buffaloes: An Opinion

Detection of estrous biomarkers in the body exudates of Kangayam cattle (Bos indicus) from interplay of hormones and behavioral expressions

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