Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: artemisia campestris (asteraceae)

Abstract

Background and objectives: A lot of research has been directed towards medicinal plants which are considered as a source of multiple phytotherapic substances endowed with hypoglycemic activities that could be used to treat diabetes and its complications. Our study was carried out in Wistar rats to investigate the hypoglycemic effect of n-Butanol Fraction from Artemisia campestris leaf Methanolic Extract (BFACME).Methods: Two experimental models were used in rats: orally induced hyperglycemia (OGTT) and isolated perfused liver (IPRL).Results: BFACME at 550 mg/kg BW dose significantly reduced fasting glucose level in normal rats as compared to controls. The decrease of glycaemia was 12.6% more significant than that obtained with the standard drug glibenclamide (10 mg/kg BW), an oral antidiabetic preparation belonging to sulfonylurea class. In OGTT model, BFACME at the highest doses of 550 and 400 mg/kg BW significantly reduced the postprandial hyperglycemic peak compared to controls. In the IPRL model, treatment with BFACME significantly decreased glucose concentrations after 30 min of perfusion with 30 mM glucose solely when insulin was present. The higher doses of BFACME lead to glucose concentration at basal level as early as 90 min, while the lowest dose does not restore this concentration even to t = 120min. The best initial glucose concentration retrieval was obtained with 0.7 mg BFACME/mL/g liver. At this dose, BFACME improves the decrease of glucose level caused by only insulin by about 18%.Conclusion: The BFACME appears to exert a hypoglycemic activity by potentiating the insulin action. Keywords: Artemisia campestris; hypoglycemic activity; isolated perfused rat liver; rats.

Analysis of polyphenols in Aegle marmelos leaf and ameliorative efficacy against diabetic mice through restoration of antioxidant and anti‐inflammatory status

“…After 12 hr fasting, all the mice were fed with glucose (2 g/kg, b.w.). The blood was collected from the tail vein at 0, 30, 60, 90, and 120 min, and blood glucose levels were monitored using a glucometer (Accu‐Chek, India) (Belgacem et al., 2019).…”

Antioxidant Activity and Inhibition of Carbohydrate Digestive Enzymes Activities of Artemisia campestris L.

“…In other words, our extracts have an inhibitory effect against α-amylase and α-glucosidase in vivo, which confirmed by the fact that hydrolysis effect of these enzymes inhibited in vitro significantly by AcAE and AcEE. In orally induced hyperglycemia model, the n-butanol Fraction from Tunisian Artemisia campestris L. at the highest doses of 550 and 400 mg/kg.bw significantly reduced the postprandial hyperglycemic peak compared to control [20]. Two other Moroccan Asteraceae species (Bubonium imbricatum Cav.…”

“…Different pharmacological studies showed that Artemisia campestris L. has antibacterial [13], antitumor [14], anti-inflammatory [15], antiplatelet [16], antispasmodic [17], antidiarrheal [18], and anti-ulcer activities [19]. Belgacem et al [20] reported that Artemisia campestris L. has a hypoglycemic impact, however no information was provided on the mechanism of action responsible for this effect. Chronic hyperglycemia induces the production of extreme amounts of reactive oxygen species in the tissues and this progression can lead to sever organ damage.…”