Analysis of polyphenols in
 Aegle marmelos
 leaf and ameliorative efficacy against diabetic mice through restoration of antioxidant and anti‐inflammatory status

Ibrahim, Mohammad; Parveen, Bushra; Zahiruddin, Sultan; Gautam, Gaurav; Parveen, Rabea; Khan, Mohammad Ahmed; Gupta, Arun Kumar; Ahmad, Sayeed

doi:10.1111/jfbc.13852

Cited by 23 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oxo-inflammatory, metabolic status, and beta-cell function are improved by quercetin and sitagliptin co-treatment (Al-Ishaq et al, 2019). Furthermore, quercetin regulates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and inhibits the actions of a tyrosine kinase inhibitor, which together improve glucose-stimulated insulin secretion and have antidiabetic effects (Ibrahim et al, 2022).…”

Section: Ppi Network Of Targeted Genes and Understanding The Ac On Me...mentioning

confidence: 99%

“…Gallic acid enhances the level of insulin and antioxidant enzymes (superoxide dismutase; SOD, glutathione; GSH, and catalase; CAT) and lowers lipid peroxidation, inflammation, oxidative stress, and glucose levels by activating the AMPK pathway and subsequently activating and also protecting beta-cell damages. Besides that, it can inhibit gluconeogenic genes like phosphoenolpyruvate carboxykinase and glucose 6phosphatase and activate the protein kinase B (PKB or Akt) pathway, lowering levels of glycaemia and insulin resistance ( ; Silveira et al, 2018) (Ibrahim et al, 2022;Silveira et al, 2019).…”

Section: Ppi Network Of Targeted Genes and Understanding The Ac On Me...mentioning

confidence: 99%

See 1 more Smart Citation

Integrating phytochemical profiling, in vitro studies and network pharmacology to reveal the antidiabetic action mechanism of Punica granatum fruit peel

Ibrahim¹,

Tiwari²,

Fahim³

et al. 2022

NRFHH

Self Cite

View full text Add to dashboard Cite

Despite considerable studies, diabetes continues to affect millions of people worldwide with an increasing rate of morbidity and mortality. Therefore, we aimed to explore the in vitro antidiabetic and antioxidant effects of Punica granatum and also reveal its drug-likeness, toxicity and action mechanism by utilizing SwissADME (absorption, distribution, metabolism and excretion), ProTox-II and network pharmacology, respectively. The results showed that P. granatum has a significant dose-dependent inhibition potential against α-amylase (IC50=131.90 ± 0.44) and α-glucosidase (IC50=149.74 ± 0.58) activity, as well as a significant inhibition in DPPH-free radical scavenging (IC50=108.57 ± 0.52) activity. ADME/Toxicity prediction of the compounds satisfies Lipinski’s rule of five with zero violations and did not find any toxicity. Moreover, network pharmacology revealed that polyphenolic compounds of P. granatum may combat diabetes by acting on key targets such as IRS1, TNF-α, IL6, MAPK3, DPP4, LEPR, GSK3B, PPARA and PIK3CG were strongly involved in glucose metabolism, oxo-inflammatory responses and insulin-related pathways. Based on the finding, we can conclude that P. granatum might be promising therapeutics for the management of diabetes and related disorders.

show abstract

Section: Ppi Network Of Targeted Genes and Understanding The Ac On Me...mentioning

confidence: 99%

Section: Ppi Network Of Targeted Genes and Understanding The Ac On Me...mentioning

confidence: 99%

Integrating phytochemical profiling, in vitro studies and network pharmacology to reveal the antidiabetic action mechanism of Punica granatum fruit peel

Ibrahim¹,

Tiwari²,

Fahim³

et al. 2022

NRFHH

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the strong interaction of gymnemic acid with α-amylase and α-glucosidase suggested that G. sylvestre reduced carbohydrate digestion and absorption. It is also reported that these compounds controlled insulin levels in the pancreatic beta-cells by enhancing the 5′ adenosine monophosphateactivated protein kinase pathway [32,33]. Furthermore, previous ndings also suggested that gymnemic acid may control oxo-in ammations, insulin resistance, and glucose metabolism, which are bene cial for the of diabetes.…”

Section: Molecular Docking Analysismentioning

confidence: 92%

Fusion, Micronization and Bio-activated (FMB) extract of Gymnema sylvestre for higher antidiabetic and antioxidant effects compared to its conventional standardized extract

Ibrahim¹,

Gokarn²,

Tamoli³

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Despite considerable studies, diabetes continues to remain a challenge for its prevention and management with an increasing rate of morbidity and mortality. One of the popular herbal extracts - Gymnema sylvestre has been extensively studied for its anti-diabetic potential. The present study aimed to explore the in vitro antidiabetic and antioxidant effects of Gymnema sylvetsre (Gudmar; GD) and also reveal its drug-likeness, toxicity and action mechanism by utilizing in silico molecular docking and network pharmacology, respectively. The elemental analysis confirmed that FMB technology successfully replenishes iron, zinc and calcium in the conventional extract which is devoid of these essential trace elements. GD extract-FMB (IC50 = 131.90 ± 0.44) are more effective as compared to conventional GD extract in the case of α-amylase (IC50 = 118.23 ± 0.42; IC50 = 109.52 ± 0.34) and α-glucosidase (IC50 = 109.52 ± 0.34), respectively while a similar effect was observed in DPPH-free radical scavenging (IC50 = 129.2 ± 0.98) of GD extract-FMB as compared to GD extract (IC50 = 176.6 ± 0.51). Network pharmacology revealed that key target proteins that are strongly linked to iron, zinc and calcium were strongly involved in glucose metabolism, oxo-inflammatory responses and insulin-related pathways. In addition, in silico molecular docking results of gymnemic acid against α-amylase and α-glucosidase revealed strong biological bindings. Conclusively, based on the finding we can conclude that FMB technique replenish the ETEs in the conventional extract. Also FMB processing is beneficial and provides a innovative solution for the herbal pharmaceutical industry to develop better efficacious extracts or herbal formulations.

show abstract

“…Last but not least, the ionization process, source of ionization, and flighting distance and time need to be adjusted and favored also based on the chemical nature and complexity of the metabolites. Moreover, the repeatability of the optimized or non-optimized chromatographic and spectroscopic methods needs to be validated to reproduce robustness [ 31 ].…”

Section: Review Findingsmentioning

confidence: 99%

Metabolomics and Network Pharmacology in the Exploration of the Multi-Targeted Therapeutic Approach of Traditional Medicinal Plants

Sharma

Yadav

2022

Plants

View full text Add to dashboard Cite

Metabolomic is generally characterized as a comprehensive and the most copious analytical technique for the identification of targeted and untargeted metabolite diversity in a biological system. Recently, it has exponentially been used for phytochemical analysis and variability among plant metabolites, followed by chemometric analysis. Network pharmacology analysis is a computational technique used for the determination of multi-mechanistic and therapeutic evaluation of chemicals via interaction with the genomes involved in targeted or untargeted diseases. In considering the facts, the present review aims to explore the role of metabolomics and network pharmacology in the scientific validation of therapeutic claims as well as to evaluate the multi-targeted therapeutic approach of traditional Indian medicinal plants. The data was collected from different electronic scientific databases such as Google Scholar, Science Direct, ACS publication, PubMed, Springer, etc., using different keywords such as metabolomics, techniques used in metabolomics, chemometric analysis, a bioinformatic tool for drug discovery and development, network pharmacology, methodology and its role in biological evaluation of chemicals, etc. The screened articles were gathered and evaluated by different experts for their exclusion and inclusion in the final draft of the manuscript. The review findings suggest that metabolomics is one of the recent most precious and effective techniques for metabolite identification in the plant matrix. Various chemometric techniques are copiously used for metabolites discrimination analysis hence validating the unique characteristic of herbal medicines and their derived products concerning their authenticity. Network pharmacology remains the only option for the unique and effective analysis of hundreds of chemicals or metabolites via genomic interaction and thus validating the multi-mechanistic and therapeutic approach to explore the pharmacological aspects of herbal medicines for the management of the disease.

show abstract

Analysis of polyphenols in Aegle marmelos leaf and ameliorative efficacy against diabetic mice through restoration of antioxidant and anti‐inflammatory status

Cited by 23 publications

References 46 publications

Integrating phytochemical profiling, <i>in vitro</i> studies and network pharmacology to reveal the antidiabetic action mechanism of <i>Punica granatum</i> fruit peel

Integrating phytochemical profiling, <i>in vitro</i> studies and network pharmacology to reveal the antidiabetic action mechanism of <i>Punica granatum</i> fruit peel

Fusion, Micronization and Bio-activated (FMB) extract of Gymnema sylvestre for higher antidiabetic and antioxidant effects compared to its conventional standardized extract

Metabolomics and Network Pharmacology in the Exploration of the Multi-Targeted Therapeutic Approach of Traditional Medicinal Plants

Contact Info

Product

Resources

About