Exploiting exopolysaccharides from lactic acid bacteria

Jolly, Laure; Vincent, Sébastien J. F.; Duboc, Philippe; Neeser, Jean‐Richard

doi:10.1007/978-94-017-2029-8_26

Cited by 65 publications

(74 citation statements)

References 28 publications

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“…EPS from microbial sources can be classified into two groups based on their monosaccharide composition and biosynthetic pathway [11]. The homopolysaccharides (HoPS) embody dextran, mutan, alternan, reuteran, pullulan, levan, inulin, curdlan etc.…”

Section: Exopolysaccharides (Eps)mentioning

confidence: 99%

Potentials of Exopolysaccharides from Lactic Acid Bacteria

2011

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Recent research in the area of importance of microbes has revealed the immense industrial potential of exopolysaccharides and their derivative oligosaccharides from lactic acid bacteria. However, due to lack of adequate technological knowledge, the exopolysaccharides have remained largely under exploited. In the present review, the enormous potentials of different types of exopolysaccharides from lactic acid bacteria are described. This also summarizes the recent advances in the applications of exopolysaccharides, certain problems associated with their commercial production and the remedies.

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Section: Exopolysaccharides (Eps)mentioning

confidence: 99%

Potentials of Exopolysaccharides from Lactic Acid Bacteria

2011

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“…Introducing new or existing glycosyltransferases into LAB [4] or gene shuffling with glycosyltransferases are effective means for controlling EPS structure [5]. Masja et al [23] reported that heterologous production of the pneumococcal serotype 14 polysaccharide in Lactococcus lactis resulted in the recombinant product secreted into culture medium, which simplified downstream processing.…”

Section: Discussionmentioning

confidence: 99%

“…cremoris Ropy352 and showed the specificities of polymerization and export enzymes blocking the function of just one glycosyltransferase abolished the production of ropy EPS. Heterologous expression of glycosyltransferases has been found to result in different sugars added at strategic positions in generating EPSs with new properties [5].…”

Section: Discussionmentioning

confidence: 99%

“…Based on the available genetic information, genetic modification of eps genes should lead to EPSs with a different repeating unit or with a different chain length [5]. Manipulation of genes which function in export, polymerization, and determination of chain length during EPS biosynthesis were also proved effective for altering EPS structure [6].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of an Ebosin derivative produced by heterologous gene replacement in

Zhang

Shan²,

Bao

et al. 2014

Microb Cell Fact

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Background: Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp. 139 and evidenced to possess an anti-rheumatic arthritis activity in vivo. The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production. Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.Results: This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g). In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin. It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS). In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin. Conclusions: The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments. This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.

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“…cremoris were extensively studied in the last years (Cerning, 1995). EPS synthesized by LAB play a major role in the manufacturing of fermented dairy products (Duboc and Mollet, 2001;Jolly et al, 2002). These molecules are economically important because they can impart functional effects to foods and confer beneficial health effects .…”

Section: Introductionmentioning

confidence: 99%