Exploitation of Skin Microbiota in Wound Healing: Perspectives During Space Missions

Marvasi, Massimiliano; Monici, Monica; Pantalone, Desirée; Cavalieri, Duccio

doi:10.3389/fbioe.2022.873384

Cited by 7 publications

(6 citation statements)

References 66 publications

(96 reference statements)

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“…Although there are no studies on WH in humans during space missions, the well-known pathophysiological changes induced by spaceflight could affect the ability of the organism to respond effectively to injuries ( Kirkpatrick et al, 2009 ). For example, the deficient immune function ( Crucian et al, 2018 ), chronic low-grade inflammation (LGI) and metabolic alterations ( Strollo et al, 2018 ), changes in hemorrhage evolution ( Kirkpatrick et al, 2009 ), and skin microbiota ( Marvasi et al, 2022 ) could be contributing causes of healing impairment in space.…”

Section: Wound Healing In Unloading Conditionsmentioning

confidence: 99%

Role of fibroblasts in wound healing and tissue remodeling on Earth and in space

Cialdai

Risaliti²,

Monici³

2022

Front. Bioeng. Biotechnol.

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Wound healing (WH) and the role fibroblasts play in the process, as well as healing impairment and fibroblast dysfunction, have been thoroughly reviewed by other authors. We treat these topics briefly, with the only aim of contextualizing the true focus of this review, namely, the microgravity-induced changes in fibroblast functions involved in WH. Microgravity is a condition typical of spaceflight. Studying its possible effects on fibroblasts and WH is useful not only for the safety of astronauts who will face future interplanetary space missions, but also to help improve the management of WH impairment on Earth. The interesting similarity between microgravity-induced alterations of fibroblast behavior and fibroblast dysfunction in WH impairment on Earth is highlighted. The possibility of using microgravity-exposed fibroblasts and WH in space as models of healing impairment on Earth is suggested. The gaps in knowledge on fibroblast functions in WH are analyzed. The contribution that studies on fibroblast behavior in weightlessness can make to fill these gaps and, consequently, improve therapeutic strategies is considered.

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Section: Wound Healing In Unloading Conditionsmentioning

confidence: 99%

Role of fibroblasts in wound healing and tissue remodeling on Earth and in space

Cialdai

Risaliti²,

Monici³

2022

Front. Bioeng. Biotechnol.

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“…The external xator, amongst others, is the treatment of choice for space applications to manage all types of fractures in a hostile environment with restricted conditions, few resources and no health care facility to deal with complications. A major concern is taking care of soft tissue (especially in open fractures) and wound healing, which could be impaired in space conditions by increasing cell apoptosis, in ammation and decreasing matrix formation 40,41 . While allowing preservation of fracture hematoma, external xation is also less invasive thereby decreasing bleeding, infection risk and comorbidities.…”

Section: Discussionmentioning

confidence: 99%

Adequacy of In-Mission Training to Treat Tibial Shaft Fractures in Mars Analog Testing

Manon,

Saint-Guillain,

Pletser

et al. 2023

Preprint

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Long bone fractures are a concern in long-duration exploration missions (LDEM) where crew autonomy will exceed the current Low Earth Orbit paradigm. Current crew selection assumptions require extensive complete training and competency testing prior to flight for off-nominal situations. Analog astronauts (n=6) can be quickly trained to address a single fracture pattern and then competently perform the repair procedure. An easy-to-use external fixation (EZExFix) was employed to repair artificial tibial shaft fractures during an inhabited mission at the Mars Desert Research Station (Utah, USA). Bone repair safety zones were respected (23/24), participants achieved 79.2 % repair success, and median completion time was 50.04 minutes. Just-in-time training in-mission was sufficient to become autonomous without pre-mission medical/surgical/mechanical education, regardless of learning conditions (p>0.05). Similar techniques could be used in LDEM to increase astronauts’ autonomy in traumatic injury treatment and lower skill competency requirements used in crew selection.

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“…These factors explain the radiation-induced reduction in microbial diversity. In addition, the injury recovery process can be accelerated by activating the aryl hydrocarbon receptor (AhR) in keratinocytes and restoring the skin microbiome on the wound ( Uberoi et al., 2021 ; Marvasi et al., 2022 ). This reflects the protective role of cutaneous microbiota in the maintenance of the epidermis.…”

Section: Discussionmentioning

confidence: 99%

Exploring the alterations and function of skin microbiome mediated by ionizing radiation injury

Huang

et al. 2022

Front. Cell. Infect. Microbiol.

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BackgroundRadiation-induced skin injury (RISI) is still the most common and severe side effect of radiotherapy. The role of the skin’s microbial barrier in the pathogenesis and progression of RISI needs to be fully investigated.MethodsThis study aimed to explore the alterations in and functions of the skin microbiota in RISI. We applied the unculturable approach to characterize the cutaneous microbiomes of a radiation-induced animal model by sequencing the V1–V3 regions of the 16S ribosomal RNA (rRNA) gene. Combined with the downloaded clinical data of patients, a comprehensive analysis was performed to identify potential radioprotective species and metabolic pathways.ResultsThere were no significant differences in the alpha diversity indices (Sobs, Shannon, Simpson, Ace, and Chao) between the acute radiation injury and control groups. Phylum-level analysis of the RISI microbiomes exhibited significant predominance of Firmicutes (mean abundance = 67%, corrected p = 0.0035). The high abundance of Firmicutes was significantly associated with rapid healing of RISI (average relative abundance = 52%; Kruskal–Wallis: p = 5.7E−4). Among its members, Streptococcus, Staphylococcus, Acetivibrio ethanolgignens group, Peptostreptococcus, Anaerofilum, and UCG-002 [linear discriminant analysis (LDA) > 3, p < 0.05] were identified as the core genera of Firmicutes. In addition, Lachnosiraceae and Lactobacillus occupied an important position in the interaction network (r > 0.6, p < 0.05). The differential metabolic pathways of RISI were mainly associated with carbohydrate metabolism (butanoate and propanoate metabolism), amino acid metabolism (tryptophan and histidine metabolism), energy metabolism, and lipid metabolism (fatty acid degradation and biosynthesis).ConclusionThis study provides new insights into the potential mechanism and skin microbial changes in the progression of RISI. The overwhelming predominance of members of Firmicutes, including Streptococcaceae, Staphylococcaceae, Lachnospiraceae, and Lactobacillus, is potentially related to rapid healing of RISI. The microbiota–metabolite axis plays a critical role in RISI and provides promising therapeutic targets for the treatment of adverse side effects.

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Exploitation of Skin Microbiota in Wound Healing: Perspectives During Space Missions

Cited by 7 publications

References 66 publications

Role of fibroblasts in wound healing and tissue remodeling on Earth and in space

Role of fibroblasts in wound healing and tissue remodeling on Earth and in space

Adequacy of In-Mission Training to Treat Tibial Shaft Fractures in Mars Analog Testing

Exploring the alterations and function of skin microbiome mediated by ionizing radiation injury

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