Exploitation of food feedstock and waste for production of biobutanol

Abstract

Patáková P., Lipovský J., Čížková H., Fořtová J., Rychtera M., Melzoch K. (2009): Exploitation of food feedstock and waste for production of biobutanol. Czech J. Food Sci., 27: 276-283.Nine strains of solventogenic clostridia including the species C. acetobutylicum, C. beijerinckii, C. pasteurianum, and C. saccharoperbutylacetonicum were tested for the solvents production using potato, maize, and sugar beet molasses as substrates. The solvent concentrations reached in the cultivations with maize and molasses media (15.23 g/l and 13.70 g/l, respectively) looked promising. Based on the screening experiments, the strain C. acetobutylicum DSM 1731 was selected for further experiments in the laboratory bioreactor using the maize medium. The results achieved in this batch cultivation (total solvents concentration 12.91 g/l, the yield from maize starch 22%, the solvents formation productivity 0.22 g/l/h, and the ratio of B:A:E approximately 2:1:0) imply the potential of maize as an energetic crop for the biofuel production. In addition, whey protein concentrate was tested as a possible replacement of the usual but expensive media components, i.e. yeast autolysate and/or trypton, and it was confirmed that these substitutes functioned well in the glucose medium.

Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum

“…E. coli and B. megaterium were grown in Luria-Bertani broth (pH adjusted to 7.5). C. pasteurianum was grown in reinforced clostridial medium (RCM) (Merck, Germany) and modified TYA medium with glucose (Patáková et al 2009). …”

Co-fermentation of hemicellulose and starch from barley straw and grain for efficient pentoses utilization in acetone–butanol–ethanol production

“…The cost of feedstock can raise the end product price by up to 40% (Patáková et al, 2009). Therefore, the selection of a convenient process together with the minimization of the expenses for the cultivation media preparation is of key importance.…”

Draft Genome Sequence of Clostridium pasteurianum NRRL B-598, a Potential Butanol or Hydrogen Producer

“…This strain has been used in only a few studies (2–7); however, it might be a useful platform for further genetic modification because it is not sensitive to oxygen, has versatile sugar-fermenting and proteolytic abilities, seems to be genetically stable in comparison with other clostridia, and tolerates minor changes in fermentation conditions.…”