Co-fermentation of hemicellulose and starch from barley straw and grain for efficient pentoses utilization in acetone–butanol–ethanol production

Yang, Ming; Kuittinen, Suvi; Zhang, Junhua; Vepsäläinen, Jouko; Keinänen, Markku; Pappinen, Ari

doi:10.1016/j.biortech.2014.12.005

Cited by 52 publications

(17 citation statements)

References 36 publications

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“…On the one hand the plants are used as fertilizers, since they enhance the transport of oxygen to greater depths in the soil, stimulate oil-degrading microorganisms and provide microbial access to soil micropores for better degradation [142]. However, on the other hand the barley grown on contaminated sites can be used for biomass based bioprocesses such as the production of biogas or biofuels, since barley is known as substrate for biogas [143][144][145] and biofuel [146][147][148] production. Whether barley grown in these conditions would be fit for human consumption or for use as animal feed is a matter which will require further investigation.…”

Section: Discussionmentioning

confidence: 99%

From oil spills to barley growth – oil‐degrading soil bacteria and their promoting effects

Mikolasch¹,

Reinhard²,

Alimbetova

et al. 2016

J. Basic Microbiol.

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Heavy contamination of soils by crude oil is omnipresent in areas of oil recovery and exploitation. Bioremediation by indigenous plants in cooperation with hydrocarbon degrading microorganisms is an economically and ecologically feasible means to reclaim contaminated soils. To study the effects of indigenous soil bacteria capable of utilizing oil hydrocarbons on biomass production of plants growing in oil-contaminated soils eight bacterial strains were isolated from contaminated soils in Kazakhstan and characterized for their abilities to degrade oil components. Four of them, identified as species of Gordonia and Rhodococcus turned out to be effective degraders. They produced a variety of organic acids from oil components, of which 59 were identified and 7 of them are hitherto unknown acidic oil metabolites. One of them, Rhodococcus erythropolis SBUG 2054, utilized more than 140 oil components. Inoculating barley seeds together with different combinations of these bacterial strains restored normal growth of the plants on contaminated soils, demonstrating the power of this approach for bioremediation. Furthermore, we suggest that the plant promoting effect of these bacteria is not only due to the elimination of toxic oil hydrocarbons but possibly also to the accumulation of a variety of organic acids which modulate the barley's rhizosphere environment.

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Section: Discussionmentioning

confidence: 99%

From oil spills to barley growth – oil‐degrading soil bacteria and their promoting effects

Mikolasch¹,

Reinhard²,

Alimbetova

et al. 2016

J. Basic Microbiol.

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“…Reported VFAs yields were twofold [12] and 10.6-fold [13] higher than for sludge fermentation alone when the mixing ratio of food waste to sludge was 50% during co-fermentation. Some studies have attributed the increased VFAs yields to the involvement of organic matter and the microbial community [14, 15]. For example, when adding different types of substrates, some major phyla, such as Firmicutes, Chloroflexi, and Proteobacteria, were significantly changed [16].…”

Section: Introductionmentioning

confidence: 99%

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Zhang

et al. 2017

Biotechnol Biofuels

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BackgroundCo-fermentation is an attractive technology for improving volatile fatty acids (VFAs) production by treatment of solid organic wastes. However, it remains unclear how the composition of different organic matters in solid waste influences the VFAs distribution, microbial community structure, and metabolic pathway during acidogenic co-fermentation. In this study, different organic wastes were added into waste activated sludge (WAS) as co-fermentation substrates to explore the impact of organic matter composition on VFAs pattern and the microbiological mechanism .ResultsAcetate was the most dominant VFA produced in all fermentation groups, making up 41.3–57.6% of the total VFAs produced during acidogenic co-fermentation under alkaline condition. With the increased addition of potato peel waste, the concentrations of propionate and valerate decreased dramatically, while ethanol and butyrate concentrations increased. The addition of food waste caused gradual decreases of valerate and propionate, but ethanol increased and butyrate was relatively stable. Some inconsistency was observed between hydrolysis efficiency and acidification efficiency. Our results revealed that starch was mainly responsible for butyrate and ethanol formation, while lipids and protein favored the synthesis of valerate and propionate. Microbial community analysis by high-throughput sequencing showed that Firmicutes had the highest relative abundance at phylum level in all fermentation groups. With 75% potato peel waste or 75% food waste addition to WAS, Bacilli (72.2%) and Clostridia (56.2%) were the dominant respective classes. In fermentation using only potato peel waste, the Bacilli content was 64.1%, while the Clostridia content was 53.6% in the food-only waste fermentation.ConclusionsAcetate was always the dominant product in acidogenic co-fermentation, regardless of the substrate composition. The addition of carbon-rich substrates significantly enhanced butyrate and ethanol accumulation, while protein-rich substrate substantially benefited propionate and valerate generation. Potato peel waste substantially favored the enrichment of Bacilli, while food waste dramatically increased Clostridia content in the sludge.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0821-1) contains supplementary material, which is available to authorized users.

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“…In brief, clostridia species can uptake a wide range of hexoses, pentoses, and oligomers through the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and/or non-PTS transport systems [66,67]. Subsequently, hexoses and pentoses [first via the pentose phosphate pathway (PPP)] are degraded to pyruvate through the Embden-Meyerhof-Parnas (EMP) pathway along with the production of ATP and NADH [68,69]. Finally, the key intermediates of acetyl-CoA and butyryl-CoA are converted into oxidized products (i.e., acetone, acetate, or CO 2 ) or reduced products (i.e., butanol, ethanol, or butyrate) via six key enzymes (thiolase, 3-hydroxybutyryl-CoA dehydrogenase, crotonase, butyryl-CoA dehydrogenase, butyraldehyde dehydrogenase, and butanol dehydrogenase) [70] (Fig.…”

Section: Carbon Metabolismmentioning

confidence: 99%

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

Huang

et al. 2020

Biotechnol Biofuels

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The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource as it has a high energy content and is less hygroscopic than other candidates. At present, the biobutanol route, employing acetone-butanolethanol (ABE) fermentation in Clostridium species, is not economically competitive due to the high cost of feedstocks, low butanol titer, and product inhibition. Based on an analysis of the physiological characteristics of solventogenic clostridia, current advances that enhance ABE fermentation from strain improvement to product separation were systematically reviewed, focusing on: (1) elucidating the metabolic pathway and regulation mechanism of butanol synthesis; (2) enhancing cellular performance and robustness through metabolic engineering, and (3) optimizing the process of ABE fermentation. Finally, perspectives on engineering and exploiting clostridia as cell factories to efficiently produce various chemicals and materials are also discussed.

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Co-fermentation of hemicellulose and starch from barley straw and grain for efficient pentoses utilization in acetone–butanol–ethanol production

Cited by 52 publications

References 36 publications

From oil spills to barley growth – oil‐degrading soil bacteria and their promoting effects

From oil spills to barley growth – oil‐degrading soil bacteria and their promoting effects

Novel insight into the relationship between organic substrate composition and volatile fatty acids distribution in acidogenic co-fermentation

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

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