Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation

Yasuhara, Hajime; Neupane, Debendra; Hayashi, Kazuyuki; Okamura, Mitsu

doi:10.1016/j.sandf.2012.05.011

Cited by 280 publications

(154 citation statements)

References 17 publications

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“…In addition, these findings are similar to those for sand cemented by enzymatically induced carbonate precipitation. 28) Therefore, our results indicate that the Pararhodobacter sp. strain SO1, which originates from the sand near the beachrock, has the potential for soil improvement.…”

Section: 9)mentioning

confidence: 62%

Microbially Induced Sand Cementation Method Using <i>Pararhodobacter</i> sp. Strain SO1, Inspired by Beachrock Formation Mechanism

Danjo

Kawasaki

2016

Mater. Trans.

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To develop an alternative ground improvement technique in coastal areas based on bio-stimulation, we investigated sand cementation using bacteria that have been shown to enhance beachrock formation. We conducted cementation tests using Pararhodobacter sp. strain SO1, a local ureolytic bacteria originating from the sand near beachrock in Okinawa, Japan. Specifically, we attempted to cement sand specimens to unconfined compressive strength (UCS) of several MPa and establish the influence of several test conditions (curing temperature, injection interval of cementation solution, Ca 2+ concentration and sodium malate concentration in the cementation solution, and test period) on the UCS. Column specimens were cemented up to UCS of 10 MPa after 28 days under the conditions (curing temperature; 30°C, injection interval; 1 day, Ca 2+ concentrations in cementation solution; 0.3 M). Multiple regression analysis showed that the relevant conditions for UCS were test period, D (days), and Ca 2+ concentration of the cementation solution, C ca (M). The prediction formula for UCS, q ud (MPa), was experimentally determined to be q ud = 48.3C ca + 0.456D ¹ 19.51. Overall, the results of this study will contribute to the application of a new technique for coastal sand improvement and bio-stimulation.

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Section: 9)mentioning

confidence: 62%

Microbially Induced Sand Cementation Method Using <i>Pararhodobacter</i> sp. Strain SO1, Inspired by Beachrock Formation Mechanism

Danjo

Kawasaki

2016

Mater. Trans.

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“…Sword beans (Canavalia gladiate) urease is an example. The permeability as well as strength characteristics have been examined using sword beans urease [104]. The permeability of the improved samples has been reduced by more than one order of magnitude.…”

Section: Caco 3 Precipitationmentioning

confidence: 99%

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

Ran¹,

Kawasaki²

2016

J Civil Environ Eng

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Geotechnical and geo-environmental engineering applications currently being explored through bio mineralization process include cementation of sands to improve mechanical properties and hence, to enhance bearing capacity and liquefaction resistance, sequestration of carbon, soil erosion control via surficial stabilization, groundwater flow control and remediation of soil and groundwater impacted by metals and radionuclides. Bio mediated system broadly refers to a chemical reaction series that are managed and controlled through biological activities and byproducts resulting from those reactions alter the properties of material in the system. Bio mediated methods such as microbial induced calcite precipitation (MICP), biofilm formation, biogas generation and biopolymers have been developed by injecting or stimulating microbes. MICP is the most widely explored method. Microbes having urease activity enhance the hydrolysis of urea and it helps to control the pH and to precipitate carbonate. Although it is a widely explored bio mediated method it has some disadvantages.The plant-derived urease may offer many benefits over microbial urease to induce carbonate cementation. Therefore, it is advantageous to explore the knowledge regarding such a technique as an eco-friendly method for different applications in the fields of geoenvironmental/geotechnical Engineering. The main objective of this paper is to provide an overview of the importance of plant-derived urease for different applications in the fields of geoenvironmental/ geotechnical engineering. The information presented in this paper may be important for biotechnologists/geoengineers to have wide ranging updates on the current situation.

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“…Calcite precipitation techniques have been proposed as the alternative method for improving the engineering properties of soil [1][2][3][4]. Enzyme-mediated calcite precipitation (EMCP) may be one of the promising methods [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…Enzyme-mediated calcite precipitation (EMCP) may be one of the promising methods [4][5][6][7][8]. The unconfined compressive strength of treated sand ranging from 200 kPa to 1.6 MPa, depending upon the amount of precipitated calcite can be achieved, and the permeability of the improved samples can be reduced by more than one order of magnitude [4,5,9]. In this technique, an enzyme of urease is employed to dissociate the urea into ammonium and carbonate ions.…”

Section: Introductionmentioning

confidence: 99%

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Applicability of Natural Zeolite for NH-Forms Removal in Enzyme-Mediated Calcite Precipitation Technique

2017

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This study evaluated the applicability of natural zeolite for the removal of the NH-forms in the enzyme-mediated calcite precipitation technique. The natural zeolite of mordenite was added to prepared grouting solutions composed of urea and urease and mixed thoroughly using a rotation table for the mixing times of 0.5, 1.0, and 2.0 h. Then, the concentrations of evolving NH-forms in the solutions were measured. The effects of the presence of zeolite on the amount and the mineralogical substance of the precipitated minerals were also evaluated by X-ray powder diffraction and scanning electron microscopy analyses. Sand samples were treated with the grouting solutions containing zeolite, and the improvement in strength was assessed. It was found that utilizing zeolite in grouting solutions can reduce the concentration of NH-forms. A significant reduction in the concentration of NH-forms was obtained. The addition of 10 g natural zeolite/L solution, combined with the 2-h mixing time, resulted in removal efficiencies of 75% and 45% in reagent concentrations of 0.5 and 1.0 mol/L, respectively. Mechanical test results showed that the grouting solutions also brought about a significant improvement in the soil strength. A precipitated material, comprising 9% of the sand mass, was produced by three pore volume (PV) injections of the grouting materials, which showed an unconfined compressive strength of 300 kPa.

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Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation

Cited by 280 publications

References 17 publications

Microbially Induced Sand Cementation Method Using <i>Pararhodobacter</i> sp. Strain SO1, Inspired by Beachrock Formation Mechanism

Microbially Induced Sand Cementation Method Using <i>Pararhodobacter</i> sp. Strain SO1, Inspired by Beachrock Formation Mechanism

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

Applicability of Natural Zeolite for NH-Forms Removal in Enzyme-Mediated Calcite Precipitation Technique

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