Experimentelle Untersuchungen an menschlichen Leberpunktaten und Rattenleberschnitten zur Oxidation von Fettsäuren mit unterschiedlicher Kettenlänge und unterschiedlicher Zahl von Doppelbindungen

Abstract

Summary.A method to determine laC02 formed from albumin bond 1-14C fatty acids in human liver biopsies is described. The method was developed mostly using rat liver slices.Independent upon chain length and number of double bonds fatty acids were oxidized proportional to their concentration in the medium. Short chain and long chain fatty acids did not influence each other. Glucose only depressed the oxidation of short chain fatty acids. Ethanol, on the other hand, decreased the oxidation of long chain and short chain fatty acids.In human liver biopsies the determination of the molar oxidation rates showed the dominating influence of the chain length. Similar results were obtained with rat liver slices. A maximal rate was found for the C a fatty acid. The long chain fatty acids showed the lowest oxidation rates. With the exception of the stearic acid they disclosed only minor differences. Stearie acid, which was oxidized much more easily, showed ATP formation rates comparable to those of short chain fatty acids.In view of the small size of the patient groups data were not correlated to diseases and diets. Both factors, however, were of minor importance in comparison to the influence of the type of the fatty acid.

The metabolism of lipids in mouse pancreatic islets. The oxidation of fatty acids and ketone bodies

“…The general pattern of fatty acid oxidation by the B-cells was similar to that in other tissues in that the rate of oxidation showed an inverse relationship to the chain length of the fatty acid (Bjorntorp, 1968;Scheig, 1968;Fischel & Oette, 1974). It should be noted, however, that in vivo octanoate may be of minor importance, since this fatty acid constitutes only a small amount of the dietary fat in adult mammals.…”

Experimentelle Untersuchungen an menschlichen Leberpunktaten und an der Ratte zum Cholesterin- und Cholesterinester-Stoffwechsel mit unterschiedlichen Vorläufern in Abhängigkeit vom Kohlenhydrat-Fett-Gehalt in der Nahrung

“…In vorangehenden Arbeiten konnte gezeigt werden, dab ErhShungen der Fetts£urekonzentration im Inkubationsmedium mit einer gesteigerten Fet~s£ureaufnahme in die Lipide und mit einer gesteigerten Oxidation einhergehen [5,20]. Damit stellte sieh die Frage, ob 1. damit parallel sieh aus Fetts~ureabbauprodukten vermehrt Choles~erin bildet nnd 2. eine verstgrkte Aufnahme der Fetts/~ureaktivR/it in die Cholesterinester stat~finder.…”