Experimental Verification of CAPN1 and CAST Gene Polymorphisms in Different Generations of Da-Heng Broilers

Abstract

The micromolar calcium activated neutral protease (CAPN1) and calpastatin (CAST) have been widely regarded as genes related to muscle growth and meat tenderness. The objective of this study was to verify the association of SNPs of CAPN1 and CAST genes with carcass and tenderness traits and search the possible change patterns of SNPs in CAPN1 and CAST genes in six generations of broiler breeding process for growth rate, efficiency, and reproduction, during the third generation and the ninth generation, respectively. We found that, for CAPN1, genetic effects between SNPs (G3535A, C7198A) and meat tenderness were similar in different generations, while SNP3 (G7324A) was a novel polymorphism and had significant association with carcass and tenderness traits (P < 0.05) in this study. Furthermore, there was significant association between SNP4 (G9950A) and carcass indexes instead of tenderness traits (P < 0.05) which was consistent in the two generations. Moreover, although SNP6 (G37868A) of CAST had no relevance to carcass traits or tenderness traits in the third generation, it showed significant association with LW and CW in the ninth generation (P < 0.05).

Genetic Diversity and Phyletic Evolution of Eleven Chinese Indigenous and Three Commercial Chicken Breeds by mtDNA Sequences

“…Observing this result, we think that long-term artificial selection may lead to reduced nucleotide diversity in these commercial chicken breeds. Although the DH is a commercial chicken breed, it was bred by Sichuan Dahen Poultry Breeding Co., Ltd, located at Hanchang Town, Chengdu City of Sichuan Province, using Chinese indigenous breeds, and only had a short-term artificial selection (Zhou et al, 2017). The PXY maybe suffered a longterm or high-strength artificial selection in the genetic resources conservation procedure.…”

Keragaman Gen Calpastatin pada Ayam Kampung

“…Teknik PCR yang dilakukan mengacu pada Zhou et al (2017) dengan menggunakan primer CAST forward 5'-CCAAAAGTAGATGAACATTCT-3' dan reverse 5'-GCTTCTATTAATTCCTACT-3'. Sampel DNA sebanyak 45 yang didapat dari tiga jenis ayam kampung disequencing dengan menggunakan mesin sekuenser (ABI Prims 3100-Avant Genetic Analyzer) pada fragmen primer forward dan reverse.…”

Genetic Variation in the Calpastatin Gene and its Association with Growth Traits in Batur Sheep

“…Some studies have been revealed the associations of the CAST polymorphism with carcass traits and meat quality in many livestock [15]; [16]; [17]; [18]; [19] and [19]. Recently, some of the SNPs in the CAST gene have been included as commercial genetic markers by some livestock industries [20]. It should be noted, that the existence of a similar association in sheep has been reported in only a few research papers to date [21]; [13]; [14].…”