Thirty lactating ewes were used to evaluate the effect of superovulation on milk production. Twelve ewes had been injected, prior to mating, with 700 IU of pregnant mare serum gonadotropin; 18 ewes were injected with saline as a control. Thirteen ewes (nine control and four superovulated ewes) were fed at low plane of nutrition; the other ewes (nine control and eight superovulated ewes) were fed at high plane of nutrition. Superovulated ewes, fed at both low and high planes of nutrition, had dramatically higher milk yields (59%), and their milk composition was not changed. Plane of nutrition increased milk lactose and P contents without significant effect on milk production. The increased milk yields in the superovulated ewes were accompanied by increases in dry matter, gross energy intakes, and gross efficiency of milk synthesis. At the end of lactation, superovulated ewes had higher mammary dry fat-free tissue, total DNA, and total RNA. The results demonstrated that superovulation prior to mating dramatically increased milk production and efficiency regardless of plane of nutrition. Increased milk production and efficiency in the superovulated ewes were due to the increased mammary secretory cell numbers and their synthetic activities presumably through the increased endogenous hormonal stimulation of mammary growth and development during pregnancy.
Twenty Pasundan cows were used in this study to know the effect of synchronization using prostaglandins and hormone gonadotropins on the picture of erythrocytes, leukocytes, and pasundan cow hemoglobin levels during pregnancy. The Pasudan heifers experimented with estrus using a combination of prostaglandin hormone (PGF2α) as much as 5 ml per head and gonadotropin realising hormone (GnRH) as much as 2.5 ml per head intramuscular to homogenize fertility conditions and improve fertility. The estrus mother cow is immediately carried out artificial insemination as much as 2 times with an interval of 6 hours. All test cows were given forage basalt food and adlibitum drinking water. Pregnancy examination is carried out on the 60th day and the 150th day of post-insemination using the rectal palpation method. Changes observed in the form of hematological concentrations include erythrocytes, leukocytes, and hemoglobin. The results showed that the concentration erythrocytes, leucocyte, and hemoglobin for Garut region respectively was 6.24±0.61 (million/μl); 11.54±0.25 (thousand/(μl); 11.54±0.61 (g/dl) higher than Bogor in a row was 5.99 ± 0.64 (million/μl); 11.46± 1.41 (thousand/(μl); 11.13 ± 0.60 (g/dl)). The results of the variance analysis showed that the synchronization of estrus with prostaglandins (PGF2α) and the gonadotropin releasing hormones (GnRH) did not differ markedly (P>0.05), between the concentrations of erythrocytes, leucocytes, and hemoglobin during the gestation period with cows that were not pregnant. It concluded that the synchronization treatment of estrus with prostaglandins (PGF2α) and gonadotropin releasing hormones (GnRH) had no effect on the profile of hematological concentrations during the gestational period (60 days and 150 days) and was no different from Pasundan heifers that are not pregnant.
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