The power output of wind farms depends strongly on spatial turbine arrangement, and the resulting turbulent interactions with the atmospheric boundary layer. Wind farm layout optimization to maximize power output has matured for small clusters of turbines, with the help of analytical wake models. On the other hand, for large farms approaching a fully-developed regime in which the integral power extraction by turbines is balanced through downwards transport of mean kinetic energy, the influence of turbine layout is much less understood. The main goal of this work is to study the effect of turbine layout on the power output for large wind farms approaching a fullydeveloped regime. For this purpose we employ an experimental setup of a scaled wind farm with one-hundred porous disk models, of which sixty are instrumented with strain gages. Our experiments cover a parametric space of fifty-six different layouts for which the turbine-area-density is constant, focusing on different turbine arrangements including non-uniform spacings. The straingage measurements are used to deduce surrogate power and unsteady loading on turbines for each layout. Our results indicate that the power asymptote at the end of the wind farm depends on the layout in different ways. Firstly, for layouts with a relatively uniform spacing we find that the power asymptote in the fully developed regime reaches approximately the same value, similarly to the prediction of available analytical models. Secondly, we show that the power asymptote in the fully-developed regime can be lowered by inefficient turbine placement, for instance when a large number of the turbines are located in the near wake of upstream turbines. Thirdly, our experiments indicate that an uneven spacing between turbines can improve the overall power output for both the developing and fully-developed part of large wind farms. Specifically, we find a higher power asymptote for a turbine layout with a significant streamwise uneven spacing (i.e. a large streamwise spacing between pairs of closely spaced rows that are slightly staggered). Our results thereby indicate that such a layout may promote beneficial flow interactions in the fully-developed regime for conditions with a strongly prevailing wind direction.Wind turbines are clustered in farms to provide the largest possible cumulative power, within available surface and cost. Inevitably, when turbines are closely spaced together, the momentum deficit in wakes from upstream turbines reduces the available power for downstream ones, while increased turbulence levels result in higher unsteady loading of turbine components. Depending on turbine location, operational control, and inflow conditions, wake induced power losses can be as high as 50%, compared to a lone standing turbine [1].An important aspect for wind farm design is therefore to better understand the relation between turbine layout, and the resulting wake losses and structural loading.Analytical wake models that describe downstream advection and expansion of turbine wakes [2...