Experimental Use of Fibrin Glue to Induce Site-Directed Osteogenesis from Cultured Periosteal Cells

Isogai, Noritaka; Landis, William J.; Mori, Ryuji; Gotoh, Y.; Gerstenfeld, Louis C.; Upton, Joseph; Vacanti, Joseph P.

doi:10.1097/00006534-200003000-00019

Cited by 91 publications

(63 citation statements)

References 25 publications

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“…7c). Insular areas of calcistudies, periosteal cells were cultured in medium supplemented with 50 µg/ml ascorbic acid (19,23). A convenfication were present with von Kossa staining (Fig.…”

Section: Discussion Bone Engineering Using the Bovine Periosteal Cellmentioning

confidence: 99%

“…7c). In 2000, Isogai et al (19) studied fibrin glue/periosteal cell constructs and reported results stripped periosteum can also be effective for bone for- solve problems of bone resorption after implantation.…”

Section: Discussion Bone Engineering Using the Bovine Periosteal Cellmentioning

confidence: 99%

“…(7,32) and cell-gel constructs often become smaller with time (19). Santavirta et al (30) investigated cell attachment to PGA fibers and reported that some cells To treat bone loss that is induced by disease or wounds, bone grafts have been used.…”

Section: Introductionmentioning

confidence: 99%

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Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

et al. 2006

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To treat bone loss that is induced by disease or wounds, bone grafts are commonly used. In dentistry, guided tissue regeneration is effective in the treatment of periodontal diseases. However, bone resorption after implantation is a major problem with the bone graft and guided tissue regeneration technique. This study examines a cell pellet culture system without exogenous scaffolds for bone regeneration. First, we examined the effect of ascorbic acid on cells. Transmission electron microscopic observation revealed that cells formed a three-dimensional structure of multiple cell layers after 5 weeks of culturing in medium containing 50 µg/ ml ascorbic acid with the medium changed every 7 days. A single cell pellet was produced by centrifuging cells that were gathered from 10 tissue culture dishes. Van Gieson staining and collagen type I immunostaining showed that the pellet contained collagen fibers and cells that adhered to the collagen fibers. Several of these cell pellets were implanted subcutaneously on the backs of nude mice for 6 weeks. Histology and immunohistochemistry results indicated new bone formation, vascular invasion, and insular areas of calcification. Bone tissue was surrounded by osteoblasts. The appearance of new bone formation is similar to that seen in intramembranous ossification. The present pellet system is reliable and might solve problems of bone resorption after implantation.

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“…7c). Insular areas of calcistudies, periosteal cells were cultured in medium supplemented with 50 µg/ml ascorbic acid (19,23). A convenfication were present with von Kossa staining (Fig.…”

Section: Discussion Bone Engineering Using the Bovine Periosteal Cellmentioning

confidence: 99%

Section: Discussion Bone Engineering Using the Bovine Periosteal Cellmentioning

confidence: 99%

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Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

et al. 2006

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“…Periosteal cells have been widely used in bone tissue engineering by isolating osteoprogenitor cells from the patient's periosteum and seeding them into bioresorbable scaffolds [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Fetal bone cells for tissue engineering

Montjovent

Burri

Mark

et al. 2004

Bone

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We envision the use of human fetal bone cells for engineered regeneration of adult skeletal tissue. A description of their cellular function is then necessary. To our knowledge, there is no description of human primary fetal bone cells treated with differentiation factors. The characterization of fetal bone cells is particularly important as the pattern of secreted proteins from osteoblasts has been shown to change during aging. In the first part of this work, human primary fetal bone cells were compared to adult bone cells and mesenchymal stem cells for their ability to proliferate and to differentiate into osteoblasts in vitro. Cell proliferation, gene expression of bone markers, alkaline phosphatase (ALP) activity, and mineralization were analyzed during a time-course study. In the second part of this paper, bone fetal cells behavior exposed to osteogenic factors is further detailed. The doubling time of fetal bone cells was comparable to mesenchymal stem cells but significantly shorter than for adult bone cells. Gene expression of cbfa-1, ALP, a1 chain of type I collagen, and osteocalcin were upregulated in fetal bone cells after 12 days of treatment, with higher inductions than for adult and mesenchymal stem cells. The increase of ALP enzymatic activity was stronger for fetal than for adult bone cells reaching a maximum at day 10, but lower than for mesenchymal stem cells. Importantly, the mineralization process of bone fetal cells started earlier than adult bone and mesenchymal stem cells. Proliferation of fetal and adult bone cells was increased by dexamethasone, whereas 1a,25-dihydroxyvitamin D 3 did not show any proliferative effect. Mineralization studies clearly demonstrated the presence of calcium deposits in the extracellular matrix of fetal bone cells. Nodule formation and calcification were strongly increased by the differentiation treatment, especially by dexamethasone. This study shows for the first time that human primary fetal bone cells could be of great interest for bone research, due to their fast growth rate and their ability to differentiate into mature osteoblasts. They represent an interesting and promising potential for therapeutic use in bone tissue engineering.

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“…They have been used in conjunction with other biomaterials such as coral or hydroxyapatite to heal bone defects [20,21]. It has been demonstrated that fibrin glue can be used as a carrier for calvarial osteoprogenitor cells to repair critical sized osseous facial defects [22] and for periosteal cells to induce heterotropic osteogenesis in rat experiments [23]. It has also been reported that fibrin glue together with periosteum derived osteoprogenitor cells can heal segmental bone defects in rabbits [9].…”

Section: Page 4 Of 35mentioning

confidence: 99%

Engineering New Bone via a Minimally Invasive Route Using Human Bone Marrow-Derived Stromal Cell Aggregates, Microceramic Particles, and Human Platelet-Rich Plasma Gel

Chatterjea

Yuan

Chatterjea

et al. 2013

Tissue Engineering Part A

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Tissue Engineering Part A eering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel (doi: 10.1089(doi: 10. /ten.TEA.2012 This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction. The final published version may differ from this proof. 2 AbstractThere is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell based bone tissue engineered constructs rely on solid pre-formed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques.The aim of this study was to develop a clinically relevant, easily moldable, bone tissue engineered construct (TEC), amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSC) and calcium phosphate micro particles in combination with an in-situ forming platelet rich plasma (PRP) gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro prior to seeding them on the scaffolds as unaggregated MSCs did not generate any bone. 24 hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to pre-formed scaffoldsemployed through an open surgery for treating bone defects. Page 2 of 35Tissue Engineering Part A eering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel

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Experimental Use of Fibrin Glue to Induce Site-Directed Osteogenesis from Cultured Periosteal Cells

Cited by 91 publications

References 25 publications

Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

Periosteal Cell Pellet Culture System: A New Technique for Bone Engineering

Fetal bone cells for tissue engineering

Engineering New Bone via a Minimally Invasive Route Using Human Bone Marrow-Derived Stromal Cell Aggregates, Microceramic Particles, and Human Platelet-Rich Plasma Gel

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