Experimental Trypsin-induced Urothelial Cell Separation<i>in vivo</i>

Gardiner, Robert A.; Masters, J. R. W.; Molland, E. A.

doi:10.1111/j.1464-410x.1980.tb08920.x

Cited by 2 publications

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References 2 publications

(1 reference statement)

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“…Such treatment with trypsin at the concentration used has previously been shown to produce rapid extensive urothelial shedding and presumably loss of the associated gag layer. 19 Recently, Engler et al 20 reported that pretreatment of the bladder with ethanol was effective for improving adenoviral -mediated gene transfer and expression in the bladder urothelium, consistent with our results indicating the importance of the initial removal of the gag layer before intravesical gene therapy. Further studies in our laboratory will focus on methods to remove the gag layer using reagents Cancer Gene Therapy, Vol 7, No 12, 2000 WATANABE, SHINOHARA, SAZAWA, ET AL: HUMAN BLADDER CANCER CELL MODEL that can be used in human intravesical trials, because the results to date strongly suggest that such a strategy will be required to obtain a high level of gene transfer in superficial bladder tumors by intravesical instillation.…”

Section: Discussionsupporting

confidence: 90%

An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies

et al. 2000

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Orthotopic implantation of human bladder cancer cells into immunodeficient mice is an important tool for studying the biology and effects of therapy. Nevertheless, the incidence of tumor implantation and growth by transurethral instillation of the human bladder cancer cells into murine bladders has been low or not reproducible. However, using a modified intravesical technique and the human bladder cancer cell lines, KU -7 and UM -UC -2, we have been able to obtain a high and reproducible incidence of superficial bladder tumors. Furthermore, intravesical administration of the LacZ adenovirus vector resulted in significant -galactosidase expression in these bladder tumors as well as the normal urothelium, which was associated with the removal of the glycosoaminoglycan layer. Because this modified technique produces a high incidence of superficial human tumor growth and allows the efficacy of gene transfer to be evaluated, it should be a useful model for the study of intravesical gene therapy for human bladder cancer. Cancer Gene Therapy ( 2000 ) 7, 1575 ± 1580

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Section: Discussionsupporting

confidence: 90%

An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies

et al. 2000

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“…2 and 3). The proteolytic enzyme, trypsin, can be used to separate bladder epithelium for in vitro studies (2,20), and Gardiner et al (9) reported the trypsin-induced urothelial cell separation in and observation of urothelial regeneration. The trypsinization which does not cause the disaggregation of cell layers lower than intermediate cells, was employed to remove superficial cells from normal mouse bladder epithelium (Figs.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Mouse Model of Cystitis and Roles of Type 1 Fimbriated Escherichia coli in Its Pathogenesis

Liao

Tomochika

Watanabe

et al. 1992

Microbiology and Immunology

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The role of type 1 fimbriae in promoting bladder colonization and the course of Escherichia coli cystitis were examined with type 1 fimbriated strains of clinically isolated E. coli. In the experiments of mice in vivo, intact bladder epithelium showed natural resistance to the adherence of type 1 fimbriated and nonfimbriated E. coli. However, the exfoliation of bladder superficial cells by trypsinization before the bacterial inoculation promoted the adhesion and colonization of type 1 fimbriated E. coli onto bladder epithelium. After colonization of E. coli, maximum numbers of E. coli and leukocytes were observed 3 days after inoculation.Nine days after inoculation, both of E. coli and leukocytes disappeared and the regeneration of superficial cells was observed.On the other hand, superficial cells in mice injected with phosphate-buffered saline or non-fimbriated E. coli regenerated 5 days after trypsinization.The present study demonstrated that the removal of superficial cells is essential for the adhesion and colonization of type 1 fimbriated E. coli onto bladder epithelium in vivo and a new model of E. coli cystitis in mice was established.The model which we established is valuable for histopathological, immunological, and therapeutic studies.

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Experimental Trypsin-induced Urothelial Cell Separationin vivo

Cited by 2 publications

References 2 publications

An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies

An improved intravesical model using human bladder cancer cell lines to optimize gene and other therapies

Establishment of a Mouse Model of Cystitis and Roles of Type 1 Fimbriated Escherichia coli in Its Pathogenesis

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