Experimental transmission of sheep pulmonary adenomatosis to a goat

Sharp, JM; Angus, KW; Jassim, FA; Scott, F. E. T.

doi:10.1136/vr.119.10.245

Cited by 21 publications

(10 citation statements)

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“…Exogenous JSRV RNA was found only in tumors, lung fluid, and draining lymph nodes of SPA-affected sheep, which is in agreement with results of an earlier immunological study, which concluded that epithelial tumor cells are major sites of replication for JSRV (27). Lung fluid and tumor tissues from SPA-affected sheep are the only materials that have been used successfully to reproduce the tumor experimentally in sheep and goats (7,24,36,37,42). These findings, therefore, point to exogenous JSRV as a strong candidate for the etiological agent of SPA, although they do not rule out possibilities such as reactivation of an enJSRV as a downstream event of neoplasia.…”

Section: Discussionsupporting

confidence: 87%

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep

Palmarini

Cousens

Dalziel

et al. 1996

J Virol

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Sheep pulmonary adenomatosis ([SPA] ovine pulmonary carcinoma) is a transmissible lung cancer of sheep that has been associated etiologically with a type D-and B-related retrovirus (jaagsiekte retrovirus [JSRV]). To date it has been impossible to cultivate JSRV in vitro and therefore to demonstrate the etiology of SPA by a classical approach. In addition, the presence of 15 to 20 copies of endogenous JSRV-related sequences (enJSRV) has hampered studies at the molecular level. The aim of this study was to investigate whether the expression of exogenous JSRV was specifically associated with neoplasia in SPA-affected animals. Initially, we found that enJSRVs were transcribed in a wide variety of normal sheep tissues. Then, by sequencing part of the gag gene of enJSRV we established a ScaI restriction site in gag as a molecular marker for the exogenous form of JSRV. Restriction enzyme digestion of PCR products obtained from the amplification of cDNA from a total of 65 tissues collected from SPA-affected and unaffected control sheep revealed that the exogenous form of JSRV was exclusively and consistently present in tumor tissues and lung secretions of the affected animals. In addition, exogenous JSRV provirus was detected only in DNA from SPA tumors and not from nontumor tissues of the same animals. This study has demonstrated clearly that the exogenous form of JSRV is specifically associated with SPA tumors.

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Section: Discussionsupporting

confidence: 87%

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep

Palmarini

Cousens

Dalziel

et al. 1996

J Virol

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“…The disease can also be efficiently transmitted to goats by experimental inoculation [77,81]. JSRV has been definitively demonstrated as the aetiological agent of OPA by experimental inoculation of particles produced from a JSRV-molecular clone [57].…”

Section: Natural Historymentioning

confidence: 99%

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

et al. 2007

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-Jaagsiekte Sheep Retrovirus (JSRV) is a betaretrovirus infecting sheep. This virus is responsible for a pulmonary adenocarcinoma, by transformation of epithelial cells from the bronchioli and alveoli. This animal cancer is similar to human bronchioloalveolar cancer (BAC), a specific form of human lung cancer for which a viral aetiology has not yet been identified. JSRV interacts with target cells through the membrane receptor Hyal2. The JSRV genome is simple and contains no recognised oncogene. It is now well established that the viral envelope protein is oncogenic by itself, via the cytoplasmic domain of the transmembrane glycoprotein and some domains of the surface glycoprotein. Activation of the PI3K/Akt and MAPK pathways participates in the envelopeinduced transformation. Tumour development is associated with telomerase activation. This review will focus on the induction of cancer by JSRV.

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“…OPA was found in goats in the 1960s, mainly in India (40)(41)(42)(43). Some experimental evidence on the induction of OPA in goat kids was also obtained in the 1980s (44,45). However, there is a paucity of reports of naturally occurring OPA in goats as opposed to those for sheep.…”

Section: Discussionmentioning

confidence: 99%

Host Species Barriers to Jaagsiekte Sheep Retrovirus Replication and Carcinogenesis

Caporale

Martineau

Heras

et al. 2013

J Virol

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f Understanding the factors governing host species barriers to virus transmission has added significantly to our appreciation of virus pathogenesis. Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA), a transmissible lung cancer of sheep that has rarely been found in goats. In this study, in order to further clarify the pathogenesis of OPA, we investigated whether goats are resistant to JSRV replication and carcinogenesis. We found that JSRV induces lung tumors in goats with macroscopic and histopathological features that dramatically differ from those in sheep. However, the origins of the tumor cells in the two species are identical. Interestingly, in experimentally infected lambs and goat kids, we revealed major differences in the number of virus-infected cells at early stages of infection. These differences were not related to the number of available target cells for virus infection and cell transformation or the presence of a host-specific immune response toward JSRV. Indeed, we also found that goats possess transcriptionally active endogenous retroviruses (enJSRVs) that likely influence the host immune response toward the exogenous JSRV. Overall, these results suggest that goat cells, or at least those cells targeted for viral carcinogenesis, are not permissive to virus replication but can be transformed by JSRV.

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Experimental transmission of sheep pulmonary adenomatosis to a goat

Cited by 21 publications

References 0 publications

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep

The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

Host Species Barriers to Jaagsiekte Sheep Retrovirus Replication and Carcinogenesis

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