Experimental Tooth Movement into New Bone Area Regenerated by Use of Bone Marrow–Derived Mesenchymal Stem Cells

Tanimoto, Kotaro; Sumi, Keisuke; Yoshioka, Mariko; Oki, Nanae; Tanne, Yuki; Awada, Tetsuya; Kato, Yukio; Sugiyama, Masaru; Tanne, Kazuo

doi:10.1597/12-232

Cited by 23 publications

(31 citation statements)

References 20 publications

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“…We developed an alveolar bone regeneration method in patients with CL/P using human bone marrow mesenchymal stem cells (hBMSCs). The transplantation of hBMSC regenerated bone in a canine alveolar cleft model and orthodontic tooth movement was achieved in the regenerated bone (Sumi et al, ; Tanimoto et al, ; Yoshioka et al, ). However, puncture of the iliac bone is still necessary for bone marrow aspiration.…”

Section: Introductionmentioning

confidence: 99%

Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration

et al. 2020

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Objectives Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about the bone‐regenerative effects of stem cells from human exfoliated deciduous teeth (SHED) and conditioned media (CM). Our aim was to address this. Materials and methods Stem cells were isolated from primary tooth pulp and cultured. Defects were made in calvariae of immunodeficient mice and implanted with stem cell‐ or CM‐containing atelocollagen. Regenerated bone was analysed by microcomputed tomography, haematoxylin–eosin and Masson's trichrome staining. Vascular endothelial growth factor, CD31 and CD34 expression were confirmed by immunohistochemistry, and the presence of several proteins and growth factors was verified in SHED‐CM. Results Bone regeneration was enhanced in defects treated with stem cells and CM compared to that in controls 8 weeks after transplantation. Mature bone formation and angiogenesis were confirmed with CM but not with stem cells or in controls. Secretome analysis using multiple cytokine assays revealed that SHED‐CM contained tissue‐regenerating factors with roles in angiogenesis and osteogenesis. Conclusion CM non‐invasively regenerate bone and might be effective to reconstruct alveolar clefts in CL/P patients.

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Section: Introductionmentioning

confidence: 99%

Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration

et al. 2020

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“…MSC in combination with biomaterials, carry a great potential that has already been proven in animal studies and on first human cases. [17][18][19][20][21][22][23][24][25][26][27][28] Several aspects have to be taken into consideration when planning stem cell-enhanced bone regeneration.…”

Section: Stem Cellsmentioning

confidence: 99%

“…One of its major advantages is that maxilla-derived stem cells proliferation potential is probably unaffected by donor age, and only population doubling time seems to increase with donor age. 56 BM-MSC have already been used in the alveolar cleft regeneration on animal models [17][18][19][20][21][22] and in first clinical cases. 23-28 BM-MSC were by many investigators found suitable for craniofacial tissue engineering including calvarial bone defects 57-61 and both maxillary 62 and mandibular 63-67 bone reconstruction.…”

Section: Bone Marrow-derived Stem Cellsmentioning

confidence: 99%

“…Efficient vascularization is important for new bone formation and paracrine stem cell activity could boost it through VEGF secretion. The experiment resulted in complete closure of the cleft on the experimental site that was comparable with the original height of alveolar ridge and allowed for subsequent orthodontic tooth movement 18. Zhang et al investigated19 whether, on a canine model, autologous bone marrow-derived and osteogenically induced mesenchymal stem cells combined with porous β-TCP could repair bone defect and thus allow for succeeding orthodontic tooth movement.…”

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Stem cell regenerative therapy in alveolar cleft reconstruction

Gładysz¹,

Hozyasz²

2015

Archives of Oral Biology

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“…In our previous study, we cultured and transplanted MSCs and carbonated hydroxyapatite particles into a bone defect area and observed that early bone regeneration occurred in the presence of transplanted MSCs [5]. Another study showed that when a tooth was moved to a site of bone that had been regenerated by MSCs and carbonated hydroxyapatite particle transplantation, the movement was the same as in normal bone [6]. Thus, normal bone remodeling appears to occur in regenerated bone.…”

Section: Introductionmentioning

confidence: 98%

The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis

Oki¹,

Abe²,

Kunimatsu³

et al. 2018

biomedicalresearch

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Objective: Angiogenesis is essential for bone remodeling and regeneration. We previously showed that 3 months after transplantation of mesenchymal stem cells (MSCs) into a bone defect area, significantly more regenerated bone was present on the MSC side than on the control side. On the side transplanted with MSCs, the number of blood vessels was significantly higher. Therefore, during the remodeling process, MSCs, which are necessary for regenerating bone, were involved in bone regeneration through angiogenesis in the transplanted area. In addition, MSCs produce Vascular Endothelial Growth Factor (VEGF). Therefore, we investigated the influence of differentiation of MSCs on the expression of VEGF. Methods: We investigated how changes in osteogenic differentiation of MSCs affect VEGF gene and protein expression and how co-culture with Human Umbilical Vein Endothelial Cells (HUVECs) affects gene expression in MSCs. We also determined the role MSCs play in the transplanted area, and the effects on HUVEC morphology when co-cultured with MSCs. Results: VEGF gene and protein expression in undifferentiated MSCs was higher than in differentiated MSCs. VEGF gene expression in MSCs co-cultured with HUVECs was higher than when MSCs were cultured alone. In co-cultures of MSCs and HUVECs, a lumen-like structure was observed on the 3 rd day of culture, and then several luminal structures were observed later. Conclusion: MSC transplantation may contribute to angiogenesis via production of VEGF.

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Experimental Tooth Movement into New Bone Area Regenerated by Use of Bone Marrow–Derived Mesenchymal Stem Cells

Abstract: It is suggested that mesenchymal/carbonated hydroxyapatite transplantation therapy has great potential as a new treatment modality for bone regeneration in patients with cleft palate.

Cited by 23 publications

References 20 publications

Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration

Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration

Stem cell regenerative therapy in alveolar cleft reconstruction

The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis

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