Experimental Study of Tissue Graft Vascularization by Means of Vascular Implantation and Subcutaneous Burying

Shen, Tzuyao; Gillies, Sir Harold

doi:10.1097/00006534-198403000-00011

Cited by 24 publications

(5 citation statements)

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“…Various authors 1,10,14,15 have suggested a simple vascular implant model within the bone tissue. We believe that a nonpermeable wrapping is needed to exclude any blood flow from the surrounding vascularized tissues, to isolate the newly vascularized bone, and to facilitate harvesting procedures.…”

Section: Discussionmentioning

confidence: 99%

“…13 The potential role of vascular implantation in composite graft salvage has been proposed. 10 The biological basis of bone neovascularization remains unclear and undoubtedly is worthy of more detailed investigation. 25 Further points to be cleared up concern the time required for the neovascularization process.…”

Section: Discussionmentioning

confidence: 99%

“…8,9 Tissue neovascularization can be induced by vascular implanting procedures. 10 The first studies in vascular induction to the bone tissue were fundamentally aimed at overcoming the problems represented by avascular necrosis. 1,[10][11][12] This line of study has led to the development of several vascular implanting procedures, especially for Kienbock's disease.…”

mentioning

confidence: 99%

“…10 The first studies in vascular induction to the bone tissue were fundamentally aimed at overcoming the problems represented by avascular necrosis. 1,[10][11][12] This line of study has led to the development of several vascular implanting procedures, especially for Kienbock's disease. 13 On the basis of the results achieved in this field, some authors [14][15][16] have used vascular implanting procedures to obtain composite vascularized grafts for reconstruction of multiple tissue loss.…”

mentioning

confidence: 99%

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Neovascularized bone grafts: Experimental investigation

Busa

Adani²,

Castagnetti

et al. 1999

Microsurgery

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Vascularized bone grafts are standardized procedures in reconstructive surgery but there are some disadvantages: donor site morbidity, limited number of "natural" donor sites, and complex technique. In this study, we test the possibility of creating a "neovascularized" bone graft utilizing a vascular implantation procedure in a rabbit model. Sixteen New Zealand adult white rabbits were used. In each animal, two iliac crest bone grafts (7 x 7 x 10 mm) were harvested. Vascular implantation of the right superficial femoral vessels was performed in one of the two grafts, which was wrapped in a silicone envelope to avoid neovascularization from the surrounding tissues and positioned in a subcutaneous pocket in the right medial thigh. On the left side, the bone block, wrapped in the silicone envelope, was buried subcutaneously without vascular implantation. The operated animals were divided into two groups: Group I included eight rabbits explanted 4 weeks postoperatively and Group II included eight rabbits explanted 8 weeks postoperatively. Tetracycline injection was performed 72 hours preexplantation to evaluate new bone formation. Selective colloidal ink injection in the axial artery was performed to investigate the neovascularization before inclusion in poly-methyl-methacrylate (PMMA). Histological examination was performed in all explanted specimens comparatively. Histological examination 8 weeks after surgery showed a marked neovascularization, with normal bone cells. Tetracycline labeling showed new bone formation with a normal pattern. In all nonvascularized specimens, no viable cells or neovascularization and no bone formation were found. The vascular implantation procedure can induce a good neovascularization with new bone formation in a small bone graft. The possibility of neovascularization induction by the simple vascular implantation procedure has several clinical implications in reconstructive surgery.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Neovascularized bone grafts: Experimental investigation

Busa

Adani²,

Castagnetti

et al. 1999

Microsurgery

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“…They demonstrated the potential of creating a vascularized bone graft nourished by an implanted vascular bundle. Washio, 11 Erol and Spira, 12 and Tzuyao 13 reported a similar method to transfer segments of composite tissue, such as intestine and omentum. Nettelblad et al 6 reported molded vascularized osteogenesis, using titanium chambers with autogenous corticocancellous bone chips and vascular bundle implantation.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Vascularized Bone Grafts Using Ceramic Chambers

Mizumoto¹,

Inada²,

Weiland³

1993

J reconstr Microsurg

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The present study was designed to create a vascularized bone graft combining the osteogenetic potential of bone marrow cells, and vascular bundle implantation in a hydroxyapatite chamber, using New Zealand white rabbits. A hydroxyapatite chamber was molded into a cylindrical shape, and hydroxyapatite fillers were soaked in an autogenous bone-marrow-cell suspension at a concentration of 1.6 x 10(8)/ml. In one group, the implant was packed with allogenic demineralized bone matrix powder (DBM) and implanted in the mid-thigh subcutaneously, with the epigastric vessels running through the chamber. In a second group, the chamber with bone marrow cells and DBM was implanted subcutaneously, without vascular bundle implantation. The control group consisted of a chamber without DBM, soaked in venous blood and implanted in the opposite thigh, with the epigastric vessels running through the chamber. Cross-sectional bone area and mineral apposition rate were measured, in addition to newly-formed vessels. After 3 weeks, chambers implanted with bone marrow cells and DBM demonstrated consistent bone formation in the pores of the chamber walls and within the chambers. No evidence of bone formation was noted in the chambers soaked with venous blood. The results indicated that vascular-bundle implantation promoted earlier bone formation with neovascularization in the chambers with bone-marrow cells and DBM. Microangiograms revealed vascular connections between the vascular bundle and soft tissue surrounding the chamber through newly-formed vessels in the chamber wall. These findings support the concept of creating a preformed vascularized bone graft, to reconstruct segmental bone defects.

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Experimental study on the behavior of a transplanted vascular pedicle

Hung

Cheng

1989

Microsurgery

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The transparent chamber technique of the hamster cheek pouch was utilized to observe the behavior of a transplanted vascular pedicle within a relatively adverse environment. Observations included direct visualization with intravital microscopy, histology, scanning electron microscopy, and alkaline phosphatase staining for vascular endothelium. The results supported the phenomenon of vascular sprouting from the transplanted vascular pedicle.

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Experimental Study of Tissue Graft Vascularization by Means of Vascular Implantation and Subcutaneous Burying

Cited by 24 publications

References 0 publications

Neovascularized bone grafts: Experimental investigation

Neovascularized bone grafts: Experimental investigation

Fabrication of Vascularized Bone Grafts Using Ceramic Chambers

Experimental study on the behavior of a transplanted vascular pedicle

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