Experimental Study of the Possible Protective Effect of Alpha-Lipoic Acid on Paracetamol induced Oxidative Stress and Hepatic Toxicity in albino rats

Abstract

Background: Paracetamol, is the most widely used over-the-counter analgesic and antipyretic medication in the world, which has minimal adverse effects at therapeutic dosages. But in high doses causes hepatic damage and oxidative stress. Objectives: The current study was designed to investigate paracetamol toxic effects upon the liver and oxidative stress after repeated oral dose and evaluate possible protective effect of alpha lipoic acid when co-administered with and after paracetamol. Methods: forty eight white albino rats were divided equally into four groups. Each group was subdivided into two sub groups A & B. Group I received gum acacia suspension. Group II received Alpha lipoic acid (50mg/kg) orally. Group III received paracetamol (1 gm/ kg orally) for 4 weeks. Group IV received paracetamol and alpha lipoic acid at the same doses. Sub groups A were euthanized after 4 weeks, while sub groups B were euthanized after 8 weeks. Blood was collected for evaluation of liver functions and oxidative stress marker. The livers were preserved for histopathological examinations. Results: The study proved that repeated administration of paracetamol induced disturbed liver functions and oxidative stress. But this toxic effects decline markedly when alpha lipoic acid (ALA) was coadministered with paracetamol. And more improvement occurs when ALA was administered for another 4 weeks after stoppage of paracetamol. Conclusions: The present study concluded that repeated paracetamol administration has hepatotoxic and oxidative stress effect and alpha lipoic acid has a protective effect against such harmful effects especially when ALA was administered for another 4 weeks after stoppage of paracetamol.

Antioxidant Mechanism of Renal and Hepatic Failure Prevention Related to Paracetamol Overdose by the Aqueous Extract of Amblygonocarpus andongensis Stem Bark

“…The animals treated with AEAASB at doses of 250 and 500 mg/kg and the satellite test group showed normal structural characteristics of the kidneys and liver. This is in agreement with the results of Dhibi et al [ 61 ] and Allam et al [ 62 ] who showed, respectively, that Eucalyptus globulus extract prevented renal alterations and alpha-lipoic acid prevented liver damage induced by paracetamol overdose administration in rats. These results suggest that AEAASB prevented paracetamol-induced damage.…”

Study the hepatoprotective effects and oxidant-antioxidant status of beta vulgaris roots ethanolic extract in hepatotoxic rats induced by acetaminophen

“…In the present study, the results of enzymes (AST, ALT and ALP) were clearly indicated the hepatotoxic effect of the APAP at the dose used since there were a significant increase in all of these hepatic enzymes in APAP group at both periods (14 and 28 days). Elevated liver enzymes (AST, ALT and ALP) in positive control group with liver damage and cell necrosis were also reported in other studies which attributed to the accumulation of APAP toxic metabolite, namely NAPQI in liver tissues causing oxidative stress effects (45). It was reported that a toxic dose of paracetamol leads to the consumption of the stored glutathione (GSH) and sulfate.…”