Experimental Study of the Effects of Permafrost Microorganisms on the Morphofunctional Activity of the Immune System

Kalenova, L. F.; Suhovey, U. G.; Broushkov, A. V.; Melnikov, V. P.; Fisher, T. A.; Besedin, I. M.; Новикова, М. А.; Efimova, J. A.; Субботин, А. М.

doi:10.1007/s10517-011-1289-9

Cited by 9 publications

(2 citation statements)

References 2 publications

(1 reference statement)

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“…Bacillus sp. isolated from permafrost sands of the Mammoth Mountain in Central Yakutia was characterized by an extraordinary viability (about 3.5 million years old) and enhanced longevity, immunity and resistance to heat shock and UV irradiation in Drosophila melanogaster and mice [11,12,13,14]; probiotic activity by a Bacillus sp. strain isolated from the same sample has been recently reported [15].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment

Zhang

Brouchkov

Griva

et al. 2013

Biology

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In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatty acids (PLFA) demonstrated the dominance of bacteria over fungi; the analysis of fatty acids specific for Gram-positive and Gram-negative bacteria revealed an approximately twofold higher amount of Gram-negative bacteria compared to Gram-positive bacteria. Direct microbial counts after natural permafrost enrichment showed the presence of (4.7 ± 1.5) × 108 cells g−1 sediment dry mass. Viable heterotrophic bacteria were found at 0 °C, 10 °C and 25 °C, but not at 37 °C. Spore-forming bacteria were not detected. Numbers of viable fungi were low and were only detected at 0 °C and 10 °C. Selected culturable bacterial isolates were identified as representatives of Arthrobacter phenanthrenivorans, Subtercola frigoramans and Glaciimonas immobilis. Representatives of each of these species were characterized with regard to their growth temperature range, their ability to grow on different media, to produce enzymes, to grow in the presence of NaCl, antibiotics, and heavy metals, and to degrade hydrocarbons. All strains could grow at −5 °C; the upper temperature limit for growth in liquid culture was 25 °C or 30 °C. Sensitivity to rich media, antibiotics, heavy metals, and salt increased when temperature decreased (20 °C > 10 °C > 1 °C). In spite of the ligninolytic activity of some strains, no biodegradation activity was detected.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment

Zhang

Brouchkov

Griva

et al. 2013

Biology

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“…В то же время биологическое влияние бактерий из ММП как на растения, так и на другие совре-менные организмы практически не изучено. Этот вопрос рассматривается лишь в отдельных работах (Калёнова и др., 2011;Brushkov et al, 2011;Субботин и др., 2011, 2012, свидетельствующих об изменении адаптивного потенциала лабораторных животных и гидробионтов.…”

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Influence of permafrost microorganisms on morphophysiological indicators of spring wheat

Субботин¹,

Narushko²,

Bome³

et al. 2016

Vestn. VOGiS

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Effects of Low-Doses of Bacillus Spp. from Permafrost on Differentiation of Bone Marrow Cells

2015

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The effects of a new microorganism species (Bacillus spp., strain M3) isolated from permafrost specimens from Central Yakutia (Mamontova Mountain) on the bone marrow hemopoiesis were studied on laboratory mice. Analysis of the count and immunophenotype of bone marrow cells indicated that even in low doses (1000-5000 microbial cells) these microorganisms modulated hemopoiesis and lymphopoiesis activity. The percentage of early hemopoietic precursors (CD117(+)CD34(-)) increased, intensity of lymphocyte precursor proliferation and differentiation (CD25(+)CD44(-)) decreased, and the percentage of lymphocytes released from the bone marrow (CD25(+)CD44(+)) increased on day 21 after injection of the bacteria. These changes in activity of hemopoiesis were associated with changes in the level of regulatory T lymphocytes (reduced expression of TCRαβ) and were most likely compensatory. The possibility of modulating hemopoiesis activity in the bone marrow by low doses of one microorganism strain isolated from the permafrost could be useful for evaluating the effects of other low dose bacteria on the bone marrow hemopoiesis.

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Experimental Study of the Effects of Permafrost Microorganisms on the Morphofunctional Activity of the Immune System

Cited by 9 publications

References 2 publications

Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment

Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment

Influence of permafrost microorganisms on morphophysiological indicators of spring wheat

Effects of Low-Doses of Bacillus Spp. from Permafrost on Differentiation of Bone Marrow Cells

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