Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues

Economides, Nikolaos; Kotsaki-Kovatsi, V. P.; Poulopoulos, A.; Kolokuris, Ioannis; Rozos, Georgios; Shore, R.C.

doi:10.1016/s0099-2399(06)80436-x

Cited by 70 publications

(66 citation statements)

References 13 publications

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“…Sealapex is a calcium hydroxide-based root canal sealer. Calcium hydroxide sealers are generally known to have good cytocompatibility 19,20) . Regarding Sealapex, our results were not in agreement with those of previous studies, which showed that this material was noncytotoxic at 24 h after mixing 21) .…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

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The objective of this study was to assess the cytotoxicity of EndoSeal, a novel mineral trioxide aggregate-based root canal sealer in comparison with two commonly used sealers, AH Plus and Sealapex. For cytotoxicity assay, MG-63 cells and human gingival fibroblasts were incubated in culture medium containing eluates of each sealer at 1, 3, and 7 days. Cell metabolism was evaluated by the WST-1 assay. For cell adhesion assay, disc specimens were fabricated from EndoSeal and AH Plus. MG-63 cells and human gingival fibroblasts were seeded on the discs, and after 1 day and 7 days of incubation, cell morphology and cell adhesion were examined by SEM. The order of cytotoxicity of root canal sealers was as follows: EndoSeal < AH Plus < Sealapex. Both types of cells seeded on the EndoSeal specimens were much larger, flat with rough margins compared to those on the AH Plus specimens. These results suggest that EndoSeal has a satisfactory cytocompatibility.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

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“…The method is now a common preliminary biocompatibility test, and it is currently used in rats to verify the biocompatibility of implantation material in subcutaneous tissue [25,26]. In this way, researchers can evaluate the tissue response around the implants [33,34], and the test has been widely accepted for this purpose [35]. Polyethylene tubes do not induce persistent irritation, and the tested material remains in the surrounding area.…”

Section: Discussionmentioning

confidence: 99%

“…Polyethylene tubes do not induce persistent irritation, and the tested material remains in the surrounding area. Thus, they allow researchers to analyze inflammatory infiltrates [33,34,36].…”

Section: Discussionmentioning

confidence: 99%

Mixing failures of endodontic sealers: an in vivo biocompatibility study

Bueno

Lopes

Valentim

et al. 2017

BDS

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Objective: Evaluate, in vivo, the influence of mixing failures on endodontic sealers. Material and methods: To alveolus analysis, 80 rats were divided into Sealapex® and AH Plus® groups. Within each group, the sealer was subjected to either partial (incomplete homogenization—simulating handling failures) or total mixing (complete homogenization) over two periods of 7 and 30 days (n = 20). The maxillary incisor was extracted and a polyethylene tube containing the sealer was inserted. To quantify edema, 40 male rats were divided into four groups (n = 10). The animals received 2% Evans Blue intravenously, and either AH Plus® or Sealapex® was injected subcutaneously. The rats were euthanized after 3 or 6 hours and analyzed in a spectrophotometer (630 ƞm). To analyze the subcutaneous tissue, 20 rats received polyethylene tube implants with the sealers in the dorsal area (n=10), then euthanized after either 7 or 30 days, and inflammation was evaluated according to an inflammatory cells score. Results: In the alveolar 7-day group, control group presented an inflammation score 1, while all other groups presented a score 2, except AH plus® total mix group (3). After 30 days, all groups presented a score 1. The edemogenic test showed less edema in Sealapex® groups (p < 0.5). In subcutaneous 7-day period, all groups presented score 2. In 30 days, all groups revealed score 1, except AH Plus® partial mix group (2). Conclusion: Regarding mixing of the sealers, there were no significant differences among the groups.KeywordsAH Plus; Inflammation; Materials testing; Root canal filling materials; Sealapex.

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“…É importante salientar que outros tipos de acondicionadores são utilizados para essa metodologia como, por exemplo, os tubos de dentina, as cápsulas de colágeno, os tubos de silicone rígidos (ZMENER; GUGLIELMOTTI; CABRINI, 1990), e os tubos de teflon SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996; …”

Section: 1-tubo De Polietilenounclassified

“…São utilizados também tubos de teflon SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996;, de silicone rígido (ZMENER; GUGLIELMOTTI;…”

Section: Introductionunclassified

Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno. Estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico

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Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues

Cited by 70 publications

References 13 publications

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Mixing failures of endodontic sealers: an in vivo biocompatibility study

Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno. Estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico

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