Experimental study of parameters and methods of surgical treatment with 445 nm laser

Abstract

Compliance with standardized conditions for the use of lasers and the development of modes in experimental conditions is one of the main factors that make it possible to safely use a laser in surgical practice. To date, a new 445 nm blue laser has now become available for otorhinolaryngologists. The use of this type of laser in rhinosurgery requires some both experimental and clinical studies that will help signal the possibilities and limitations of the use of this laser. Objectives: Choose the safest and most sparing mode of exposure to a laser with a wavelength of 445 nm for rhinosurgery. Materials and methods: The experimental part of the study was carried out on samples of biological tissues with optical properties close to the tissues in the nasal cavity. As a biological model, preparations of chicken muscle tissue and preparations of chicken liver tissue were selected. A new laser with a wavelength of 445 nm was used. The results of the microscopic picture were evaluated by histological examination. The study was carried out using a biological microscope Biolam M-1 (JSC “LOMO”, Russia), with standard magnification of 100 times. Staining of histological preparations was carried out according to hematoxylin-eosin, trichrome according to Masson. To measure the specified parameters, the VIDEOTEST system for morphometry was used. Conclusion: A laser with a wavelength of 445 nm in the experiment showed the possibility of using it in a constant contact mode. The obtained results of the maximum depth of damage when using a laser with a wavelength of 445 nm suggest the most sparing and safe modes of using the laser in surgical practice.