Experimental studies on the influence of fibrin adhesive, factor XIII, and calcitonin on the incorporation and remodeling of autologous bone grafts

Gerngroß, H.; Burri, C.

doi:10.1007/bf00435648

Cited by 22 publications

(11 citation statements)

References 14 publications

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“…Gerngross et al noted a negative impact on bone remodeling in the cortical tibia of a sheep model upon the addition of a fibrin sealant on autologous cancellous bone grafts. 20 Lappalainen et al 21 also observed a similar result upon the association of a fibrin glue with autologous particulate bone in rabbit critical-sized calvarial defects. The negative effect was explained by the potential role of fibrin in limiting the ingrowth of vessels into the grafted bone or its role as a void filler which prevents the formation of the natural fibrin.…”

Section: Injectable Fibrin-based Tissue Engineeringmentioning

confidence: 70%

“…18,19 Variations in experimental design, implantation site, and animal models as well as fibrinogen and thrombin concentrations are other parameters that make direct comparison of the research findings difficult. Moreover, to achieve the desired biological and/or physical properties for bone tissue engineering applications, fibrin must be reinforced, and there is a board range of nano-and other materials such as autogenous bone grafts, 20,21 allogenic bone grafts, 22,23 xenogeneic bone grafts, 24 metals, [25][26][27] ceramics, [28][29][30] …”

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

“…For example, Linsley et al showed that while hMSC growth in pure fibrin scaffolds decreased with increasing fibrin concentration, in the presence of BCP ceramics, the trend was reversed and greatest cell growth was detected in the highest fibrin concentration. 72 Kim et al mixed demineralized bone powder and various fibrinogen concentrations (10,20,40, or 80 mg/mL) by 5 U/mL thrombin and showed that the proliferation and expression of osteoblastic markers of hMSCs were enhanced in scaffolds prepared from higher fibrinogen concentrations. 73 However, it is necessary to point out that there was a noticeable decrease in the growth rate of cells cultured on the scaffolds fabricated with 80 mg/mL fibrinogen, probably due to lower porosity of the scaffolds and the results may vary if the cell culture duration time increased.…”

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confidence: 99%

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A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

368

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Section: Injectable Fibrin-based Tissue Engineeringmentioning

confidence: 70%

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

mentioning

confidence: 99%

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A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

368

256

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“…29 -34 The administration of factor XIII has shown to accelerate fracture healing and increase bone stability in various different osteotomy experiments, 35,36 to promote the osseointegration/stability of implants and the healing of pseudoarthroses, 37 as well as to accelerate the incorporation and remodelling of bone grafts. 38,39 In a fibrin-alginate bead system previously evaluated for the cultivation of periosteal cells, 40 periosteal cells were cultivated after initial monolayer culture under osteogenic culture conditions and the influence of three different growth factors that support osteogenic differentiation. The fibrin beads are effective carrier structures that support the bone-and cartilage-forming potential of different types of cells that can be combined with different matrices for special requirements of cell culture.…”

Section: Introductionmentioning

confidence: 99%

Optimization of bone engineering by means of growth factors in a three‐dimensional matrix

Arnold

Schweitzer

Lindenhayn

et al. 2003

J Biomedical Materials Res

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The direction and acceleration of differentiation by administering growth factors is one of the ways of optimizing bone engineering. The present study considered the influence of the growth factors factor XIII, TGF-beta 1, and b-FGF on the proliferation and osteogenic differentiation of porcine periosteal cells in a three-dimensional carrier matrix (bead), consisting of a fibrin-alginate-hydroxyapatite composite. F XIII, TGF-beta 1, and b-FGF were added to the culture medium of monolayer culture and fibrin beads in different concentrations. The monolayer culture was assessed on the basis of cell counts, while DNA, osteocalcin, osteonectin, and collagen content and alkaline phosphatase activity were determined, and microscopic and immunohistologic evaluations were performed for the beads. In the monolayer, the addition of b-FGF led to a significantly shorter time up to the confluence of the cells. In the bead, cell proliferation was accelerated by b-FGF and TGF-beta 1. With regard to alkaline phosphatase activity, factor XIII led to significantly higher values, while b-FGF and TGF-beta 1 resulted in lower activities. Osteocalcin content was significantly increased by the application of b-FGF. For the osteonectin content the addition of growth factors did not produce any changes. The application of TGF-beta 1 during the monolayer culture significantly increased the primary collagen content of the beads. The administration of different growth factors opens up new ways of optimizing cell growth in vitro and of directing the osteogenic differentiation of periosteal cells, without one universally applicable factor having been demonstrated. It will be the task of further studies to analyze the interaction of individual factors and the chronologic dependency of the action, on the way to in vitro bone generation.

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“…We had long been using fibrin glue in lateral suboccipital craniectomy to prevent cerebrospinal fluid leakage from the mastoid air cells. An unexpected observation on CT scan, that bone fragments applied with the fibrin glue on the dural surface regrew a bone plate several months later, drew our attention to the possibility that allogenic fibrin glue could have an osteopromotive effect, even though such effects have not been seen using xenogeneic fibrin glue in animals [7,8,14,32]. This observation prompted the present study to test regeneration of bone following regrafting of bone fragments with allogenic fibrin glue in patients.…”

Section: Introductionmentioning

confidence: 94%

Osteoregenerative lateral suboccipital craniectomy using fibrin glue

Sawamura¹,

Terasaka²,

Ishii³

et al. 1997

Acta neurochir

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This report describes a simple technique of cranioplasty for suboccipital craniectomy and the results of a clinical study to assess the effects of fibrin glue on regeneration of the skull. Cranioplasty using a mixture of autologous bone chips and human allogenic fibrin glue was performed in 31 patients who received lateral suboccipital craniectomy. Long-term observations with three-dimensional CT showed satisfactory reconstruction of the mastoid-occipital bone plate in 25 patients (81%); among them, a nearly complete reconstruction of the occipital bone (plate) was found in 14 patients. Regeneration of the bone began 6 months after surgery on the inner surface, adjacent to the dura mater. In conclusion, the present technique provides a new simple method to restore an autologous bone plate in a cranial defect made by piecemeal craniectomy.

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Experimental studies on the influence of fibrin adhesive, factor XIII, and calcitonin on the incorporation and remodeling of autologous bone grafts

Cited by 22 publications

References 14 publications

A review of fibrin and fibrin composites for bone tissue engineering

A review of fibrin and fibrin composites for bone tissue engineering

Optimization of bone engineering by means of growth factors in a three‐dimensional matrix

Osteoregenerative lateral suboccipital craniectomy using fibrin glue

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