Experimental model of Border Disease Virus
infection in lambs: comparative pathogenicity
of <i>pestiviruses</i> isolated in France and Tunisia

Thabti, Fatma; Fronzaroli, L; Dlissi, Elyess; Guibert, Jean-Michel; Hammami, Salah; Pépin, Michel; Russo, Pierre

doi:10.1051/vetres:2001004

Cited by 35 publications

(31 citation statements)

References 19 publications

(16 reference statements)

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“…Such long-lasting viremia associated with pestivirus infection has only been described in chamois ). In contrast, during transient infection in sheep, viremia is short-lived, lasting no more than 12-15 days (Thabti et al, 2002). In other wild species, such as elk (Cervus elaphus), viremia was only detected between 3 dpi and 7 dpi (Tessaro et al, 1999).…”

Section: Discussionmentioning

confidence: 97%

“…After 5 dpi, leukopenia was more severe for inoculated animals than for the control chamois and lasted until the end of the experiment. Leukopenia is commonly associated with pestivirus infection in both domestic (Thabti et al, 2002) and wild species. In Pyrenean chamois, leukopenia has been associated with BDV infection in both natural (Fernández-Sirera et al, 2011) and experimental conditions .…”

Section: Discussionmentioning

confidence: 99%

“…Three virus subcultures were produced to obtain inocula. The final viral suspension was titrated before inoculation, and ETM52 cells were grown in minimum essential medium (MEMGibcoH, Invitrogen, Carlsbad, California, USA) as described by Thabti et al (2002) and used for both virus production and virus neutralization (VN) tests. Fetal calf serum used for cell cultures was confirmed to be free of pestivirus.…”

Section: Cells and Virusmentioning

confidence: 99%

“…Animals were inoculated under sedation by intratracheal injection (Thabti et al, 2002) of 5 ml of cell culture medium containing 1.0310 6 50% tissue culture infectious doses (TCID 50 ) per milliliter of the CADI-6 strain. They were inoculated during the second third of gestation, as estimated by ultrasound.…”

Section: Experimental Designmentioning

confidence: 99%

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Experimental Infection of Pregnant Pyrenean Chamois (Rupicapra Pyrenaica) With Border Disease Virus Subtype 4

Martin¹,

Duquesne²,

Guibert³

et al. 2013

Journal of Wildlife Diseases

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ABSTRACT:Border disease virus (BDV) causes high mortality in Pyrenean chamois (Rupicapra pyrenaica) on the French and Spanish sides of the Pyrenees Mountains. We investigated the pathology induced by BDV in pregnant chamois via experimental infection. Three females were inoculated during the second third of pregnancy with a BDV-4 subgroup strain isolated from a wild Pyrenean chamois during an acute epizootic. A fourth pregnant chamois and one nonpregnant ewe were kept as negative controls. Animals were monitored to assess clinical signs, hematology, viremia, and serology. Postmortem examinations included necropsy, histopathology, and quantification of viral RNA in organs. Pregnancy was unsuccessful in all inoculated animals. One died 24 days postinoculation (dpi) without showing any precursory clinical signs. The second animal had profuse diarrhea from 13 dpi to its death at 51 dpi. The third aborted at 46 dpi and was euthanized at 51 dpi. All animals were viremic from 4 dpi until death. Neutralizing antibodies against BDV-4 were detected from 12 dpi. Necropsies showed generalized lymphadenomegaly, associated in one case with disseminated petechial hemorrhages in the digestive tract. Seventyeight of 79 organs from inoculated adults and their fetuses had detectable viral RNA. The main histologic lesions in adults were mild lymphohistiocytic encephalitis associated with moderate or moderately severe lymphoid depletion. Control animals remained negative for virus (in blood and organs), antibody, and lesions upon postmortem examination. BDV infection during pregnancy in Pyrenean chamois causes severe disease leading to abortion, then death. Despite 100% fetal death following inoculation, viral RNA was recovered from all organs of infected fetuses, suggesting that persistently infected offspring could be born. Our results may help explain the reported decrease in chamois populations in several areas and suggest that great care must be taken when interpreting infection status for wildlife.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Cells and Virusmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Infection of Pregnant Pyrenean Chamois (Rupicapra Pyrenaica) With Border Disease Virus Subtype 4

Martin¹,

Duquesne²,

Guibert³

et al. 2013

Journal of Wildlife Diseases

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“…Secondly, even when samples The another reason of the higher percentage of pestiviruses in that region can be use of live vaccines purpose of control programme some viral infections for instance peste des petits ruminants, sheep and goat pox and etc. Pestiviruses are frequent contaminants of modified live virus vaccines produced on primary ovine and bovine cells 20 and may play a role in pestivirus maintenance and/ or dissemination in the region.…”

Section: Discussionmentioning

confidence: 99%

The Investigation of Pestivirus and Rift Valley Fever Virus Infections in Aborted Ruminant Foetuses in the Blacksea Region in Turkey

Albayrak¹,

Özan²

2012

Kafkas Univ Vet Fak Derg

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SummaryIn this study, aborted foetuses (cattle, sheep and goat) in either coastal or inland Blacksea region of Turkey were surveyed for the presence of nucleic acids from pestiviruses (bovine viral diarrhoea virus-BVDV, border disease virus-BDV) and rift valley fever virus (RVFV) by reverse transcriptase polymerase chain reaction assay (RT-PCR). Pestivirus nucleic acids were found in 39 of 53 aborted calves (73.6%), in 34 of 37 aborted lambs (91.9%) and in 2 of 2 aborted kids (100%) by RT-PCR. In addition RVFV has been investigated in the same foetuses, however RVFV nucleic acid has not been confirmed in any samples. Keywords: Aborted foetus, RT-PCR, Pestivirus, RVFV, Turkey Karadeniz Bölgesinde Ruminant Atık Fötüslerinde Pestivirus ve Rift Vadisi Humması Virus Enfeksiyonlarının Araştırılması ÖzetBu çalışmada, Karadeniz bölgesinin sahil ve iç kesimlerinden elde edilen atık sığır koyun ve keçi fötüsleri pestiviruslar (bovine viral diare virus-BVDV, border hastalığı-BDV) ve rift vadisi humması virus (RVFV) nükleik asitleri yönünden reverz transkriptaz polimeraz zincir reaksiyonu (RT-PZR) ile test edildi. RT-PZR ile 53 atık buzağının 39'unda (%73.6), 37 atık kuzunun 34'ünde (%91.9) ve 2 atık oğlağın tamamında (%100) pestivirus nükleik asidi tespit edildi. Ayrıca aynı atık fötusların tamamında RVFV araştırıldı, fakat hiçbirisinde RVFV nükleik asidi tespit edilemedi.

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Detection of a novel border disease virus subgroup in Tunisian sheep

Thabti¹,

Letellier²,

Hammami

et al. 2004

Arch Virol

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Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and representative reference strains of the different pestivirus groups. The antigenic study showed that the Tunisian isolates were closer to the two BDV reference strains than to the Alfort-187 and the NADL reference strains. Comparison of the nucleotide sequences of the 5'-non coding regions of all the Tunisian isolates to those of other pestiviruses have shown that these isolates were distinct from the established pestivirus species. The entire N(pro)-E2 coding sequences of four Tunisian isolates were determined and compared to other pestiviruses. Segregation of these pestiviruses based on the N(pro)-E2 region was identical to that obtained with the 5'UTR sequences. The phylogenetic tree obtained with these sequences showed that the Tunisian isolates formed a separate branch between the BDV and CSFV groups, and consequently a possible new species within the pestivirus genus. However, as indicated by the antigenic study and the host origin of the isolates, the Tunisian isolates were assigned to a novel subgroup within the BDV species.

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Experimental model of Border Disease Virus infection in lambs: comparative pathogenicity of pestiviruses isolated in France and Tunisia

Cited by 35 publications

References 19 publications

Experimental Infection of Pregnant Pyrenean Chamois (Rupicapra Pyrenaica) With Border Disease Virus Subtype 4

Experimental Infection of Pregnant Pyrenean Chamois (Rupicapra Pyrenaica) With Border Disease Virus Subtype 4

The Investigation of Pestivirus and Rift Valley Fever Virus Infections in Aborted Ruminant Foetuses in the Blacksea Region in Turkey

Detection of a novel border disease virus subgroup in Tunisian sheep

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