Experimental model of Achilles tendon injury in rats

Abstract

Brazil. Intellectual and scientific content of the study, critical revision. ABSTRACT PURPOSE:To describe an effective experimental model to study the Achilles tendon healing. METHODS:Forty male Rattus norvegicus albinus, Wistar lineage adult male weighing 250 to 300g were used for this experiment and thirty were surgically submitted to bilateral partial transverse section of the Achilles tendon. The right tendon was treated with radio waves (RF) whereas the left tendon served as control. On the third postoperative day, the rats were divided into four experimental groups consisting of ten rats each which were treated with monopolar RF adjusted to 650 kHz and 2w, for two minutes twice a week and a group of normal animals without any intervention, until they were sacrificed on the 7 th , 14 th and 28 th days, respectively. Tendons were weighed and collagen quantification was evaluated by hydroxyprolin content. RESULTS:Significant reduction in collagen content on day 7, 14 and 28 was related to control experiment to normal tendon (7 days, p<0.01; 14 e 28 days, p<0.05). CONCLUSION:The experimental model has been effective and available to be used to study Achilles tendon healing.

Effect of Ankaferd blood stopper® on tendon healing: an experimental study in a rat model of Achilles tendon injury

“…During the remodeling stage, the healing tissue is resized and reshaped with the decrease in cellularity and also the collagen and glycosaminoglycan synthesis occurs. [1] According to our results, ABS has negative effect on tendon healing. Up to date, no study was reported about the effects of ABS on tendon healing.…”

“…But in some studies, tendon healing process has been studied for as long as six weeks in rats. [1] With this present study, the stage at which the ABS affects the healing process is unclear. The euthanization time may be longer or may be studied with three-week and a six-week time periods with more animals.…”

“…[1] Tendon injuries are not only responsible for large health care costs, but they also result in lost work time and individual morbidity. [2] For adequate repair, proper visualization of the ends of the injured tendons and avoiding the postoperative adhesions and the bleeding are necessary.…”

Effects of apixaban, rivaroxaban, dabigatran and enoxaparin on histopathology and laboratory parameters in Achilles tendon injury: An in vivo study

“…Transection of the paratenon and Achilles tendon fascicles was achieved with a transverse incision approximately 5 mm proximal to the calcaneal insertion area. [ 7 ] The incised Achilles tendon was re-sutured with atraumatic USP 4/0 silk (Neosilk Ultra, İzmir, Turkey) [Figure 1a and b ]. After the primary repair of the tendon, the rats were released in the cages without immobilization.…”

The 532 nm Laser Treatment Promotes the Proliferation of Tendon-Derived Stem Cells and Upregulates Nr4a1 to Stimulate Tenogenic Differentiation

“…Thirty-six rats were divided into two groups: a control group and a 532 nm laser group. For the tendon injury model, we adopted a rat model of Achilles-tendon injury following a previously-reported method [19]. The Achilles tendon was separated from the plantaris and soleus tendons and injury were surgically induced by semi-cutting, followed by primary suture repair.…”