The effect of printing parameters (nozzle diameter, layer height, nozzle temperature, and printing speed), dimensions (wall thickness), and filament material on the crashworthiness performance of 3D-printed thin-walled multi-cell structures (TWMCS) undergoing quasi-static compression is presented. The ideal combination of parameters was determined by employing the Signal-to-Noise ratio (S/N), while Analysis of Variance (ANOVA) was utilized to identify the significant parameters and assess their impact on crashworthiness performance. The findings indicated that the ideal parameters for the specific energy absorption (SEA) consisted of a nozzle diameter of 0.6 mm, layer height of 0.3 mm, nozzle temperature of 220 °C, printing speed of 90 mm/s, wall thickness of 1.6 mm, and PLA(+) filament material. Afterward, the optimal parameters for crushing force efficiency (CFE) included a nozzle diameter of 0.8 mm, layer height of 0.3 mm, nozzle temperature of 230 °C, print speed of 90 mm/s, wall thickness of 1.6 mm, and PLA(ST) filament material. The optimum parameter to minimize manufacturing time is 0.3 mm for layer height and 90 mm/s for printing speed. This research presents novel opportunities for optimizing lightweight structures with enhanced energy absorption capacities. These advancements hold the potential to elevate passenger safety and fortify transportation systems. By elucidating the fundamental factors governing the crashworthiness of thin-walled multi-cell PLA 3D-printed tubes, this study contributes to a deeper understanding of the field.