Experimental infection with Rangelia vitalii in dogs: Acute phase, parasitemia, biological cycle, clinical-pathological aspects and treatment

Silva, Aleksandro S. Da; França, Raqueli Teresinha; Costa, Márcio Machado; Paim, Carlos Breno; Paim, Francine C.; Dornelles, Guilherme Lopes; Soares, João Fábio; Labruna, Marcelo Bahia; Mazzanti, Cinthia M.; Monteiro, Sílvia González; Lopes, Sonia T.A.

doi:10.1016/j.exppara.2011.04.010

Cited by 39 publications

(65 citation statements)

References 7 publications

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“…Morphologically, intraerythrocytic forms of R. vitalii closely resemble B. vogeli. Piroplasms are often scarce in the bloodstream, but might be more frequent in the acute phase of infection (CARINI;MACIEL, 1914;CARINI, 1948;BARROS, 2005;DA SILVA et al, 2011). The present study demonstrated that 6.8% of the samples were positive by PCR, whereas 3.9% were positive by morphological examination.…”

Section: Discussionmentioning

confidence: 55%

“…This protozoan infects dogs and is supposedly transmitted by ticks and causes a disease that is popularly known as "nambiuvú" (bloody ears) or "plague of blood". This illness has been described only in the south and southeast regions of Brazil (BRAGA, 1935;BARROS, 2004BARROS, , 2005DA SILVA et al, 2011). In a recent study conducted in southern Brazil R. vitalii was molecularly characterized (SOARES et al, 2011).…”

Section: Detection and Molecular Characterization Of Piroplasms Specimentioning

confidence: 99%

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Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Lemos

Cerqueira

Toma

et al. 2012

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii is a protozoon described from dogs in the south and southeast regions of Brazil. It is phylogenetically related to Babesia spp. that infects dogs, but data on this enigmatic parasite is still limited. The aim of this work was to detect piroplasm species in dogs in the state of Rio de Janeiro, Brazil, by 18S rRNA gene-based PCR assay, restriction fragment length polymorphism (RFLP) and sequence analyses. Of 103 dogs examined, seven (6.8%) were positive for Babesia spp. by PCR. The amplified products were digested by restriction enzymes to differentiate the Babesia species, and one sample was identified as Babesia vogeli. The pattern observed for the other six amplification products did not match with pattern described for large Babesia infecting dogs. Sequencing analysis confirmed these six samples as R. vitalii, with high homologies (99-100%) with a sequence from south Brazil. This study confirms the presence of Babesia vogeli and Rangelia vitalii circulate in domestic dogs in Teresópolis, Rio de Janeiro, Brazil.Keywords: Babesia vogeli, Rangelia vitalii, piroplasms, dogs, PCR. ResumoRangelia vitalii é um protozoário que infecta cães e foi descrito nas regiões Sul e Sudeste do Brasil. R. vitalii é filogeneticamente próxima à Babesia spp., mas dados deste misterioso parasito ainda são escassos. O objetivo deste trabalho foi detectar a presença de piroplasmas em cães naturalmente infectados no estado do Rio de Janeiro, através da amplificação do gene 18S rRNA pela PCR, clivagem com enzimas de restrição (RFLP) e caracterização genética através do sequenciamento. De 103 cães, sete (6,8%) foram positivos para Babesia spp. pela PCR. Os produtos amplificados foram digeridos por enzimas de restrição para a diferenciação das espécies de Babesia e uma amostra foi identificada como Babesia vogeli. O padrão de amplificação observado nas outras seis amostras não correspondeu ao padrão descrito para babesias que infectam cães. O sequenciamento das seis amostras confirmou ser uma espécie geneticamente idêntica a R. vitalii apresentando grande homologia (99-100%) com a sequência do sul do Brasil. Este estudo confirma a presença de Babesia vogeli e Rangelia vitalii infectando cães em Teresópolis, Rio de Janeiro, Brasil.

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Section: Discussionmentioning

confidence: 55%

Section: Detection and Molecular Characterization Of Piroplasms Specimentioning

confidence: 99%

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Lemos

Cerqueira

Toma

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…In a study by Fighera et al (2010), the main clinical signs in dogs that were naturally infected with R. vitalii were slight to severe jaundice (100%), signs of gastroenteritis (60%), vomiting (34.3%) and hemorrhage (28.6%). According to a study carried out by Silva et al (2011), dogs that were experimentally infected with R. vitalii presented fever, increased heart rate, apathy and anorexia. Weight loss and diarrhea were less frequent.…”

Section: Discussionmentioning

confidence: 99%

“…This protozoon can be seen as free forms in plasma and in erythrocytes, monocytes and endothelial cell cytoplasm, within cerebral capillaries and in the aorta and jugular vein (LORETTI & BARROS, 2005). It is easier to view the parasite during fever peaks in blood smears (PESTANA, 1910b;SILVA et al, 2011). It has been shown that the tick Amblyomma aureolatum is the vector responsible for its transmission (SOARES et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Lemos

Toma

Assad

et al. 2017

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii, a tick-borne piroplasm that infects dogs, has been recently molecularly characterized in Brazil, Uruguay and Argentina. Studies on molecular characterization of these piroplasms in different Brazilian regions are scarce. The aim of this study was to evaluate clinical and hematological changes in dogs caused by R. vitalii in the mountainous region of the state of Rio de Janeiro. Blood samples from 36 dogs were evaluated for piroplasms and hematological disorders using light microscopy and molecular analysis. Blood samples from all the animals included in this study were confirmed to be positive for R. vitalii through genetic sequencing. Clinical signspresented by 24 of the 36 dogs of the study were evaluated during appointments or hospitalization within private practice. The most frequent clinical disorders in these dogs that were naturally infected with R. vitalii were fever, spontaneous cutaneous bleeding and diarrhea. Normochromic non-regenerative anemia and thrombocytopenia were the most common hematological disorders in these R. vitalii-positive dogs and therefore should be considered in hematological evaluations on suspected cases.Keywords: Piroplasm, Rangelia vitalii, dogs, PCR, hematological evaluation. ResumoRangelia vitalii, um piroplasma transmitido por carrapatos que infecta cães, foi sendo recentemente caracterizado molecularmente no Brasil, Uruguai e Argentina. Nas diferentes regiões brasileiras são escassos os estudos acerca da caracterização molecular destes piroplasmídeos. O objetivo deste estudo foi avaliar as alterações clínicas e hematológicas em cães causadas por R. vitalii na região serrana do Rio de Janeiro. Amostras de sangue total de 36 cães foram examinadas quanto à presença de piroplasmas pela microscopia de luz, alterações hematológicas e análise molecular. Todos os cães do presente estudo foram positivos para R. vitalii através do sequenciamento genético. Dos 36 animais positivos para R. vitalii, 24 foram avaliados clinicamente. As alterações mais frequentemente observadas foram febre, sangramento cutâneo espontâneo e diarréia. Anemia normocítica normocrômica arregenerativa e trombocitopenia foram as alterações hematológicas mais observadas em cães positivos para R. vitalii, devendo ser consideradas na avaliação hematológica de cães suspeitos.

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“…Hemorrhagic processes may leads to anemia and may be due to thrombocytopenia, which represent a drop in platelet count caused by decreased platelet production, aggregation or destruction (Paim et al, 2012). Anemia is a classic laboratory finding present in canine rangeliosis (Eiras et al, 2014;Fighera et al, 2010;França et al, 2014;Lemos et al, 2017;Silva et al, 2011). The hypoproteinemia observed herein may be explained by the anorexia, weight loss (Eiras et al, 2014;França et al, 2014;Lemos et al, 2017;Paim et al, 2012), and hypoalbuminemia (Fredo et al, 2017), commonly related with canine rangeliosis.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Rangelia Vitalii in a Dog From the Curitiba Metropolitan Region, Parana State, Southern Brazil

Mongruel

Stedile

Sousa

et al. 2017

AVS

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Rangeliosis, caused by Rangelia vitalii, is known to causes apathy, intermittent fever, ear’s margin bleeding and anemia. Herein, we report the first molecular detection of R. vitalii infecting a dog from the Curitiba metropolitan region, Paraná State, southern Brazil. A four-year-old male mixed-breed neighborhood dog, presenting apathy, weight loss, and ear’s margin bleeding, was referred for clinical care at the Veterinary Teaching Hospital of the Universidade Federal do Paraná. Sequencing of the piroplasm positive sample showed ≥99% identity with R. vitalii 18S rDNA sequence deposited in GenBank. Further studies are needed to elucidate the pathogenicity, competence vector of ticks and epidemiology of this protozoa the State of Paraná.

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Experimental infection with Rangelia vitalii in dogs: Acute phase, parasitemia, biological cycle, clinical-pathological aspects and treatment

Cited by 39 publications

References 7 publications

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil

Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil

Molecular Detection of Rangelia Vitalii in a Dog From the Curitiba Metropolitan Region, Parana State, Southern Brazil

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