Molecular Detection of Rangelia Vitalii in a Dog From the Curitiba Metropolitan Region, Parana State, Southern Brazil

Mongruel, Anna Cláudia Baumel; Stedile, Simone Tostes de Oliveira; Sousa, Marlos Gonçalves; Valente, Jessica Damiana Marinho; Barbosa, Iago Carvalho; Spanhol, Viviane Campos; Sezyshta, Amanda; Weber, Luiz Felipe Silva; Vieira, Thállitha Samih Wischral Jayme; Vieira, Roberval Daiton

doi:10.5380/avs.v22i4.56936

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…R. vitalii is morphologically similar to B. vogeli on blood smears, but genetically distinct in molecular phylogeny (LEMOS et al, 2017). In the State of Paraná, south Brazil, only one study detected R. vitalii in a dog by molecular diagnosis (MONGRUEL et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

Silva

Ferreira

Maffezzolli

et al. 2019

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii infects erythrocytes, leukocytes and endothelial cells of dogs. The present study aimed to report the molecular detection confirmed by sequencing of R. vitalii in the state of Paraná, as well as describe the clinical, hematological and biochemical alterations of the infected dogs. Three sick dogs from the metropolitan area of Curitiba, PR, Brazil, underwent a physical exam, and laboratory tests included hematology, biochemistry, polymerase chain reaction (PCR), and gene sequencing. Clinical signs included apathy, anorexia, and hemorrhage. Intra-erythrocytic and extracellular piroplasms were found on peripheral blood smears from all three dogs. Blood samples from these animals were positive for Babesia sp. by PCR targeting 18S rRNA. PCR products from all three dogs were sequenced, and BLAST analysis showed that the PCR-generated sequences were highly homologous with those of R. vitalii previously reported. Hematologic findings included severe anemia, shift of neutrophils to the regenerative left, and thrombocytopenia. Serum urea levels were increased in all three dogs, and direct bilirubin levels were elevated in one dog.

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Section: Introductionmentioning

confidence: 99%

Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

Silva

Ferreira

Maffezzolli

et al. 2019

Rev. Bras. Parasitol. Vet.

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Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.

Oliveira

Yang²,

Duarte

et al. 2023

Ticks and Tick-borne Diseases

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Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil

Fernandes

Rodrigues

Valente

et al. 2021

Semina: Ciênc. Agrár.

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This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral blood), and polymerase chain reaction (PCR) for piroplasm based on the 18S rRNA gene, were performed. One dog (1/47, 2.1%; CI 95%: 0.1-11.3%) tested positive to Babesia vogeli in the polymerase chain reaction (PCR) assay for piroplasms and the resulting sequence showed 100% identity with Babesia vogeli isolates deposited in GenBank®. Co-infection with Ehrlichia spp. was also observed by direct examination (via blood smear). The clinical and hematological alterations observed in the positive animal were anorexia, dehydration, white mucous membranes, anemia and thrombocytopenia.

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Molecular Detection of Rangelia Vitalii in a Dog From the Curitiba Metropolitan Region, Parana State, Southern Brazil

Cited by 3 publications

References 8 publications

Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

Detection molecular of Rangelia vitalii in dogs from Parana State, Southern Brazil

Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.

Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil

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