Experimental infection of mice with Borna disease virus (BDV): Replication and distribution of the virus after intracerebral infection

Enbergs, H.; Vahlenkamp, Thomas W.; Kipar, Anja; Müller, Hermann

doi:10.1080/13550280152403317

Cited by 10 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of note, viral antigen was not detected in non-neuronal cells of peripheral organs in any of the alpacas or horses investigated in this study. This strict neurotropism of BoDV-1 observed in alpacas and horses coincides with previous work in naturally infected humans 33,41 and other non-reservoir hosts, 3,19,24,56 substantiating their status as dead-end hosts. This suggests that the virus is not shed by these hosts, which is supported by the absence of BoDV-1 RNA in nasal, conjunctival, and oral swabs or feces from the same alpacas in a previous study.…”

Section: Discussionsupporting

confidence: 90%

Borna disease virus 1 infection in alpacas: Comparison of pathological lesions and viral distribution to other dead-end hosts

et al. 2023

View full text Add to dashboard Cite

Borna disease is a progressive meningoencephalitis caused by spillover of the Borna disease virus 1 (BoDV-1) to horses and sheep and has gained attention due to its zoonotic potential. New World camelids are also highly susceptible to the disease; however, a comprehensive description of the pathological lesions and viral distribution is lacking for these hosts. Here, the authors describe the distribution and severity of inflammatory lesions in alpacas ( n = 6) naturally affected by this disease in comparison to horses ( n = 8) as known spillover hosts. In addition, the tissue and cellular distribution of the BoDV-1 was determined via immunohistochemistry and immunofluorescence. A predominant lymphocytic meningoencephalitis was diagnosed in all animals with differences regarding the severity of lesions. Alpacas and horses with a shorter disease duration showed more prominent lesions in the cerebrum and at the transition of the nervous to the glandular part of the pituitary gland, as compared to animals with longer disease progression. In both species, viral antigen was almost exclusively restricted to cells of the central and peripheral nervous systems, with the notable exception of virus-infected glandular cells of the Pars intermedia of the pituitary gland. Alpacas likely represent dead-end hosts similar to horses and other spillover hosts of BoDV-1.

show abstract

Section: Discussionsupporting

confidence: 90%

Borna disease virus 1 infection in alpacas: Comparison of pathological lesions and viral distribution to other dead-end hosts

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Germany (GER): BB = Brandenburg, BY = Bavaria, BW = Baden-Wuerttemberg, HE = Hesse, NI = Lower Saxony, RP = Rhineland-Palatinate, SN = Saxony, ST = Saxony-Anhalt; Switzerland (SUI): GR = Grisons, SG = St. Gall; Liechtenstein (LIE); Austria (AUT): UA = Upper Austria, ST = Styria. Cluster designations, host and geographic origin are indicated according to previously published work (Dürrwald, Kolodziejek, Herzog, & Nowotny, 2007;Dürrwald et al, 2014;Kolodziejek et al, 2005;Niller et al, 2020;Nobach et al, 2015;Schlottau et al, 2018;Weissenböck et al, 2017) it is described as strictly neurotropic and almost exclusively restricted to the CNS in incidentally or experimentally infected immunocompetent non-reservoir hosts (Bilzer, Planz, Lipkin, & Stitz, 1995;Enbergs, Vahlenkamp, Kipar, & Müller, 2001;Herzog, Kompter, Frese, & Rott, 1984;Korn et al, 2018;Liesche et al, 2019;Lipkin, Briese, & Hornig, 2011;Richt & Rott, 2001;Schlottau et al, 2018;Zwick, Seifried, & Witte, 1927). While RT-qPCR confirmed the predominantly CNS- To date, the bicoloured white-toothed shrew is the only known reservoir of BoDV-1 (Bourg et al, 2013;Dürrwald et al, 2014;Hilbe et al, 2006;Nobach et al, 2015;Puorger et al, 2010;Weissenböck et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Borna disease outbreak with high mortality in an alpaca herd in a previously unreported endemic area in Germany

Schulze

Große

Fürstenau

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

Borna disease virus 1 (BoDV‐1) is the causative agent of Borna disease, an often fatal neurologic condition of domestic mammals, including New World camelids, in endemic areas in Central Europe. Recently, BoDV‐1 gained further attention by the confirmation of fatal zoonotic infections in humans. Although Borna disease and BoDV‐1 have been described already over the past decades, comprehensive reports of Borna disease outbreaks in domestic animals employing state‐of‐the‐art diagnostic methods are missing. Here, we report a series of BoDV‐1 infections in a herd of 27 alpacas (Vicugna pacos) in the federal state of Brandenburg, Germany, which resulted in eleven fatalities (41%) within ten months. Clinical courses ranged from sudden death without previous clinical signs to acute or chronic neurologic disease with death occurring after up to six months. All animals that underwent necropsy exhibited a non‐suppurative encephalitis. In addition, six apparently healthy seropositive individuals were identified within the herd, suggesting subclinical BoDV‐1 infections. In infected animals, BoDV‐1 RNA and antigen were mainly restricted to the central nervous system and the eye, and sporadically detectable in large peripheral nerves and neuronal structures in other tissues. Pest control measures on the farm resulted in the collection of a BoDV‐1‐positive bicoloured white‐toothed shrew (Crocidura leucodon), while all other trapped small mammals were negative. A phylogeographic analysis of BoDV‐1 sequences from the alpacas, the shrew and BoDV‐1‐positive equine cases from the same region in Brandenburg revealed a previously unreported endemic area of BoDV‐1 cluster 4 in North‐Western Brandenburg. In conclusion, alpacas appear to be highly susceptible to BoDV‐1 infection and display a highly variable clinical picture ranging from peracute death to subclinical forms. In addition to horses and sheep, they can serve as sensitive sentinels used for the identification of endemic areas.

show abstract

“…In this study, the detection of the BoDV genome only in a part of the brain was attributed to the low viral load in the brain. BoDV-1 has been reported to spread to non-neural tissue via the peripheral nervous system (PNS) [ 1 , 12 , 24 , 41 , 42 ]. In this study, BoDV-1 was detected in the sciatic ganglion, trigeminal ganglion, parasympathetic ganglion, sinus node, adrenal gland, spleen, liver, and ovary.…”

Section: Discussionmentioning

confidence: 99%

Genetic stability of the open reading frame 2 (ORF2) of borna disease virus 1 (BoDV-1) distributed in cattle in Hokkaido

Sukmak

Okamoto

Ando

et al. 2021

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Borna disease virus (BoDV) is a neurotropic virus that causes several infections in humans and neurological diseases in a wide range of animals worldwide. BoDV-1 has been molecularly and serologically detected in many domestic and wild animals in Japan; however, the genetic diversity of this virus and the origin of its infection are not fully understood. In this study, we investigated BoDV-1 infection and genetic diversity in samples collected from animals in Hokkaido between 2006 and 2020. The analysis was performed by focusing on the P region of BoDV-1 for virus detection. The presence of BoDV-1 RNA was observed in samples of brain tissue and various organs derived from persistently infected cattle. Moreover, after inoculation, BoDV-positive brains were isolated from neonatal rats. The gene sequences of the P region of BoDV obtained from the rat brain were in the same cluster as the P region of the virus isolated from the original bovine. Thus, genetic variation in BoDV-1 was extremely low. The phylogenetic analysis revealed that BoDV-1 isolates obtained in this study were part of the same cluster, which suggested that BoDV-1 of the same cluster was widespread among animals in Hokkaido.

show abstract

Experimental infection of mice with Borna disease virus (BDV): Replication and distribution of the virus after intracerebral infection

Cited by 10 publications

References 27 publications

Borna disease virus 1 infection in alpacas: Comparison of pathological lesions and viral distribution to other dead-end hosts

Borna disease virus 1 infection in alpacas: Comparison of pathological lesions and viral distribution to other dead-end hosts

Borna disease outbreak with high mortality in an alpaca herd in a previously unreported endemic area in Germany

Genetic stability of the open reading frame 2 (ORF2) of borna disease virus 1 (BoDV-1) distributed in cattle in Hokkaido

Contact Info

Product

Resources

About