Experimental infection of European crustaceans with white spot syndrome virus (WSSV)

Corbel, Vincent; Zuprizal, Z.; Shi, Chengyin; Huang, Adam; Sumartono, Sumartono; Arcier, J.M.; Bonami, Jean-Robert

doi:10.1046/j.1365-2761.2001.00302.x

Cited by 88 publications

(59 citation statements)

References 19 publications

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“…Within a decade, it has become a serious threat to the shrimp culture industry throughout Asia and Latin America (Hill 2002). WSSV also infects many other crustacean species from several regions of the world (Lo et al 1996, Chang et al 1998, Kasornchandra et al 1998, Wang et al 1998, Rajendran et al 1999, Corbel et al 2001, Hossain et al 2001, Sahul-Hameed et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Escobedo-Bonilla¹,

Audoorn²,

Wille³

et al. 2006

Dis. Aquat. Org.

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In the past, strategies to control white spot syndrome virus (WSSV) were mostly tested by infectivity trials in vivo using immersion or per os inoculation of undefined WSSV infectious doses, which complicated comparisons between experiments. In this study, the reproducibility of 3 defined doses (10, 30 and 90 shrimp infectious doses 50% endpoint [SID 50 ]) of WSSV was determined in 3 experiments using intramuscular (i.m.) or oral inoculation in specific pathogen-free (SPF) Litopenaeus vannamei. Reproducibility was determined by the time of onset of disease, cumulative mortality, and median lethal time (LT 50 ). By i.m. route, the 3 doses induced disease between 24 and 36 h post inoculation (hpi). Cumulative mortality was 100% at 84 hpi with doses of 30 and 90 SID 50 and 108 hpi with a dose of 10 SID 50 . The LT 50 of the doses 10, 30 and 90 SID 50 were 52, 51 and 49 hpi and were not significantly different (p > 0.05). Shrimp orally inoculated with 10, 30 or 90 SID 50 developed disease between 24 and 36 hpi. Cumulative mortality was 100% at 108 hpi with doses of 30 and 90 SID 50 and 120 hpi with a dose of 10 SID 50 . The LT 50 of 10, 30 and 90 SID 50 were 65, 57 and 50 hpi; these were significantly different from each other (p < 0.05). A dose of 30 SID 50 was selected as the standard for further WSSV challenges by i.m. or oral routes. These standardized inoculation procedures may be applied to other crustacea and WSSV strains in order to achieve comparable results among experiments.

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Section: Introductionmentioning

confidence: 99%

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Escobedo-Bonilla¹,

Audoorn²,

Wille³

et al. 2006

Dis. Aquat. Org.

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“…It is a rod-shaped, double-stranded DNA virus affecting tissues of ectodermal and mesodermal origin in penaeids, and it results in mass mortalities. In addition, other crustaceans such as crabs, copepods, lobsters, prawns and krill act as asymptomatic carriers of the virus (Yu et al 1995, Flegel 1996, Supamattaya et al 1998, Rajendran et al 1999, Chen et al 2000, Corbel et al 2001. Dual infection of Penaeus monodon with WSSV and yellow head virus (YHV) , Sithigorngul et al 2000 has also been reported.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibodies developed for sensitive detection and comparison of white spot syndrome virus isolates in India

Anil¹,

Shankar²,

Mohan³

2002

Dis. Aquat. Org.

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Since its first report in 1994, white spot syndrome virus (WSSV) has become widespread in India. We have developed a simple, rapid and sensitive monoclonal antibody (MAb)-based immunodot test for detection of WSSV. Four MAbs of IgG isotype were produced against an Indian isolate of WSSV: 1 MAb recognised a 28 kDa viral protein while the other 3 recognised both 28 and 18 kDa proteins. The 4 MAbs recognised 4 different Indian WSSV isolates collected at different times from the east and west coasts of India, indicating antigenic uniformity of the isolates. The limit of detection of the immunodot test was 500 pg of the viral protein, which compared well with 1 step PCR and could be used to detect WSSV in shrimp Penaeus monodon with and without gross signs of white spots in the cuticle. Furthermore, the test was rapid (3 h for completion) and is suitable for further development as a simple field kit. KEY WORDS: WSSV · Monoclonal antibodies · Immunodot · India Resale or republication not permitted without written consent of the publisherDis Aquat Org 51: [67][68][69][70][71][72][73][74][75] 2002 studies (Lo et al. 1998 have indicated that results positive by the highly sensitive 2-step PCR (nested PCR) are not always associated with pond outbreaks and may have a limited value for field prognosis. However, stocking of seed positive by 1-step PCR is strongly associated with outbreaks (Withyachumnarnkul 1999). Therefore, the highest priority should be to exclude broodstock and seed with high levels of infection. This would require application of less-sensitive PCR (1-step) or an alternative screening procedure of similar sensitivity. In this context, we attempted to develop a simple and sensitive monoclonal antibody (MAb)-based assay that would be suitable for fieldlevel screening of shrimp for WSSV.MAb-based tests such as immunofluorescence (Zhan et al. 1999, Shih et al. 2001), immunoperoxidase and whole-mount tissue assays (Poulos et al. 2001) have been developed for detection of the Chinese isolate of WSSV. However, such tests are not ideally suited for field level screening by farmers. Furthermore, there is evidence that the various WSSV geographic isolates differ at the molecular level ) and in virulence ). There are possibly also several variants in any country/region due to long-term intensive shrimp culture and traffic in seed. Thus, we have produced monoclonal antibodies to the Indian isolate of WSSV for antigen characterisation and for development of a simple and sensitive farmer-level immunodot test. The performance of the test was compared with PCR for screening shrimp from WSS outbreaks. MATERIALS AND METHODSVirus collection and purification. Tiger shrimp Penaeus monodon, with gross signs of white spots in the cuticle, were collected from a WSS-outbreak farm in Kundapur on the west coast of India. WSSV infection was confirmed by histopathology and polymerase chain reaction (PCR) . The foregut, gill, and epithelial tissue from the cepahalothorax were separated from the shrimp and homogenised in T...

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“…In fact, a virus designated B2 that is morphologically similar to WSSV was described in Mediterranean crab Carcinus mediterraneus much before WSSV outbreaks were reported in Asia [13]. However, in experimental infection studies, WSSV was not able to develop in Mediterranean crab C. menas and this led Corbel and colleagues [4] to suggest that bacilliform viruses morphologically similar to WSSV found in Mediterranean crabs might be either different viruses or at least different strains.…”

Section: Disease Transmission Risk From Shrimps Exported For Human Comentioning

confidence: 99%

Shrimp Viral Diseases, Import Risk Assessment and International Trade

Karunasagar

Ababouch

2012

Indian J. Virol.

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Shrimp is an important commodity in international trade accounting for 15 % in terms of value of internationally traded seafood products which reached $102.00 billion in 2008. Aquaculture contributes to over 50 % of global shrimp production. One of the major constraints faced by shrimp aquaculture is the loss due to viral diseases like white spot syndrome, yellow head disease, and Taura syndrome. There are several examples of global spread of shrimp diseases due to importation of live shrimp for aquaculture. Though millions of tonnes of frozen or processed shrimp have been traded internationally during the last two decades despite prevalence of viral diseases in shrimp producing areas in Asia and the Americas, there is no evidence of diseases having been transmitted through shrimp imported for human consumption. The guidelines developed by the World Animal Health Organisation for movement of live animals for aquaculture, frozen crustaceans for human consumption, and the regulations implemented by some shrimp importing regions in the world are reviewed.

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Experimental infection of European crustaceans with white spot syndrome virus (WSSV)

Cited by 88 publications

References 19 publications

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Monoclonal antibodies developed for sensitive detection and comparison of white spot syndrome virus isolates in India

Shrimp Viral Diseases, Import Risk Assessment and International Trade

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