Experimental infection of bulls with Akabane virus

Parsonson, I. M.; Della-Porta, A.J.; Snowdon, W. A.; O'Halloran, M.L.

doi:10.1016/s0034-5288(18)32486-x

Cited by 16 publications

(9 citation statements)

References 14 publications

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“…This period is about 4–8 weeks of pregnancy in sheep, and about 8–14 weeks in cattle [6,8]. Akabane virus could not be detected in semen collected from viraemic bulls following an experimental infection [9]. Intra-uterine inoculation of Akabane virus in cattle at the time of artificial insemination did not result in clinical disease but most animals developed viraemia.…”

Section: Introduction Methods and Resultsmentioning

confidence: 99%

“…It is actually difficult to compare behavior between SBV and other worldwide orthobunyaviruses, as Akabane, Aino or Cache Valley viruses in semen, considering the facts that i) limited scientific data are available regarding semen shedding, ii) a quite low proportion of SBV-seropositive bulls present positive RT-PCR results, iii) the virus detection methods developed for semen need to be highly sensitive to detect the RNA genome (specific extraction protocols have been developed recently for SBV) iv) culture or isolation may not be sensitive enough for this group of RNA viruses, which could explain previously reported Akabane negative culture results [9]. Akabane virus was isolated from a number of tissues, including the reproductive tract (ovaries, uterine) and associated lymph nodes from cows slaughtered up to day 7 after intrauterine inoculation [17].…”

Section: Discussionmentioning

confidence: 99%

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Evidence of excretion of Schmallenberg virus in bull semen

Ponsart

Pozzi

Bréard

et al. 2014

Vet Res

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Schmallenberg virus (SBV) is a novel orthobunyavirus, discovered in Germany in late 2011. It mainly infects cattle, sheep and goats and could lead to congenital infection, causing abortion and fetal abnormalities. SBV is transmitted by biting midges from the Culicoides genus and there is no evidence that natural infection occurs directly between ruminants. Here, we could detect SBV RNA in infected bull semen using qRT-PCR (three bulls out of seven tested positive; 29 positive semen batches out of 136). We also found that highly positive semen batches from SBV infected bulls can provoke an acute infection in IFNAR-/- mice, suggesting the potential presence of infectious virus in the semen of SBV infected bulls.

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Section: Introduction Methods and Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evidence of excretion of Schmallenberg virus in bull semen

Ponsart

Pozzi

Bréard

et al. 2014

Vet Res

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“…In a comparable study by Parsonson et al . [16], eight bulls were infected with Akabane virus and semen samples were tested for virus by inoculation of tissue cultures and by subcutaneous injection of susceptible cattle. In that study viable virus was also not detected in any of the semen samples.…”

Section: Discussionmentioning

confidence: 99%

Schmallenberg virus detection in bovine semen after experimental infection of bulls

et al. 2013

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SUMMARYTo study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT–PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT–PCR). After SBV infection both animals in the study showed viraemia (qRT–PCR) with fever and diarrhoea. SBV RNA could be detected in semen from both animals. The highest SBV RNA concentrations in semen were found in the first week (days 4–7 post-inoculation) but concentrations were relatively low (Ct values 30–39). Viable SBV was only isolated from blood samples and not from semen or genital tissues.

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“…Recently, SBV RNA has been detected in the semen of naturally infected bulls and SBV infection was reported in calves inoculated experimentally with SBV RT-qPCR-positive semen [138-140]. These findings show that the semen of bulls naturally infected with SBV can be infectious and suggest that SBV and Akabane virus differ in terms of semen contamination since a group has reported previously that the semen from bulls infected experimentally with Akabane virus is not infectious [141]. The export of semen from countries where SBV is present might represent a risk of contamination.…”

Section: Preventionmentioning

confidence: 97%

Epidemiology, molecular virology and diagnostics of Schmallenberg virus, an emerging orthobunyavirus in Europe

Doceul

Lara

Sailleau

et al. 2013

Vet Res

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After the unexpected emergence of Bluetongue virus serotype 8 (BTV-8) in northern Europe in 2006, another arbovirus, Schmallenberg virus (SBV), emerged in Europe in 2011 causing a new economically important disease in ruminants. The virus, belonging to the Orthobunyavirus genus in the Bunyaviridae family, was first detected in Germany, in The Netherlands and in Belgium in 2011 and soon after in the United Kingdom, France, Italy, Luxembourg, Spain, Denmark and Switzerland. This review describes the current knowledge on the emergence, epidemiology, clinical signs, molecular virology and diagnosis of SBV infection.

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Experimental infection of bulls with Akabane virus

Cited by 16 publications

References 14 publications

Evidence of excretion of Schmallenberg virus in bull semen

Evidence of excretion of Schmallenberg virus in bull semen

Schmallenberg virus detection in bovine semen after experimental infection of bulls

Epidemiology, molecular virology and diagnostics of Schmallenberg virus, an emerging orthobunyavirus in Europe

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