Experimental infection in mice with Treponema hyodysenteriae

Joens, Lynn A.; Glock, Robert D.

doi:10.1128/iai.25.2.757-760.1979

Cited by 46 publications

(28 citation statements)

References 5 publications

(4 reference statements)

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“…isolated from rodents resemble B. murdochii in their biochemical reaction pattern (Trott et al, 1996b) but B. hyodysenteriae isolates identical to porcine isolates have been recovered from wild rats (Trott et al, 1996b) and mice . It has been shown experimentally that B. hyodysenteriae can be transmitted from mice to pigs (Joens, 1980) and laboratory mice inoculated with B. hyodysenteriae develop lesions similar to those described in the pig (Joens and Glock, 1979). There are occasional reports of the isolation of B. pilosicoli from wild mice but experimentally it seems difficult to infect mice without using a specialized diet (Jamshidian et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of Brachyspira spp. isolated from wild rodents

Backhans¹,

Johansson

Fellström³

2010

Environ Microbiol Rep

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The occurrence of intestinal spirochaetes of genus Brachyspira in wild rodents was studied by cultivating 209 caecal samples. Spirochaetal cultures were obtained from 83% of rats and 33% of house mice. Biochemical characterization and six different species-specific PCR methods were applied to 101 of 118 isolates and a selection of 34 brachyspiras were further studied by sequencing of the 16S rRNA gene. The results showed that isolates representing all the established biochemical phenotypes could be cultured from the rodents, including the porcine pathogens Brachyspira hyodysenteriae and Brachyspira pilosicoli. Phylogenetic studies indicated that rodents carry Brachyspira spp. that are closely related to porcine and avian isolates, as well as variants previously not described. One group of hippurate-negative rat isolates were shown to possess the 16S rRNA gene hexa(T) nucleotide segment, previously described only in B. pilosicoli and 'Brachyspira corvi', and phylogenetically they formed a sister lineage of the B. pilosicoli cluster. Furthermore, a large number of the rodents were colonized by slowly growing, non- or weakly haemolytic spirochaetes. Most of these brachyspiras were isolated at 37°C and phylogenetically they formed two separate clusters. Sequence analysis of their 16S rRNA genes indicated that the new variants of Brachyspira spp. may constitute novel species of the genus Brachyspira.

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Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of Brachyspira spp. isolated from wild rodents

Backhans¹,

Johansson

Fellström³

2010

Environ Microbiol Rep

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“…Immunization and challenge of mice. The mouse challenge studies were done as described by Joens and Glock (16). Three-week-old female CF-1 mice (Charles River) were immunized intraperitoneally (i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…The cloned antigens are compared with their corresponding native T. hyodysenteriae antigens, which were identified by using antibodies specific to each cloned antigen. One particular cloned antigen which was serologically related to the endoflagellar proteins of T. hyodysenteriae is shown to induce bactericidal antibody and to protect against challenge in a mouse model of T. hyodysenteriae infection and immunity (16).…”

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confidence: 99%

Cloning and characterization of Treponema hyodysenteriae antigens and protection in a CF-1 mouse model by immunization with a cloned endoflagellar antigen

1989

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We cloned genes that code for Treponema hyodysenteriae antigens into Escherichia coli with the purpose of identifying protective antigens for vaccine development. Three different genomic libraries were screened with various antisera reactive with T. hyodysenteriae antigens. The cloned antigens and corresponding native T. hyodysenteriae antigens were analyzed for molecular size, serum reactivity, solubility in sarcosine, and segregation during phase partitioning with the nonionic detergent Triton X-114. The results from these analyses suggested that the gene products were components of either the cytoplasmic membrane, periplasm, or endoflagella of T. hyodysenteriae. The cloned antigens were tested as vaccine candidates in a CF-1 mouse model of T. hyodysenteriae infection and immunity. Intraperitoneal injection of crude E. coli extracts containing cloned antigens did not protect mice from challenge. However, serum from mice injected with a crude extract of an E. coli clone which expressed an endoflageliar antigen killed T. hyodysenteriae in vitro. Partially purified preparations of this cloned endoflagellar antigen protected mice against oral challenge with both the homologous serotype (B204) and a heterologous serotype (B234) of T. hyodysenteriae. These results suggest that the endoflagellar proteins could be used as an effective subunit vaccine against T. hyodysenteriae. 3808 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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“…The mouse challenge studies were done as described [13], Six-week-old female OF-I mice (Iffa Credo, Lyon France) were kept under controlled conditions. Feed was removed 14 h before the first challenge and was withheld for 40 h. Two consecutive doses (24 h apart) were administered to mice by gastric intubation with 0.5 ml of a log-phase culture of treponemes: 10 6 or 10 8 cfu of S. hyodysenteriae Wt C5, 106 or l0 n cfu of S. hyodysenteriae C5 tly-, or 10 a cfu of S. innocens…”

Section: Virulence Test In Micementioning

confidence: 99%

Inactivation of aSerpula (Treponema) hyodysenteriaehemolysin gene by homologous recombination: Importance of this hemolysin in pathogenesis ofS. hyodysenteriaein mice

Huurne

Houten

Muir

et al. 1992

FEMS Microbiology Letters

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The role of Serpula hyodysenteriae hemolysin in the pathogenesis of swine dysentery was studied. The inactivation of a gene, tly, encoding a hemolysin, and the virulence of a tly− mutant in mice is reported. We obtained chromosomal integration of the distrupted tly gene via homologous recombination in a S. hyodysenteriae wild‐type strain following electroporation. A plasmid construct, in which the tly gene was disrupted by a kanamycin‐resistance gene, was used for the electroporation. The mutants obtained had reduced hemolysis indicating that the tly encoded hemolysin is not the only hemolysin produced by S. hyodysenteriae. Mice infected with the tly− mutant had fewer cecal lesions than mice infected with the wild‐type S. hyodysenteriae. From these results it was concluded that the tly encoded hemolysin may be an important virulence factor, but not the only one. It was demonstrated that gene transfer to spirochaetes can be achieved through electroporation.

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Experimental infection in mice with Treponema hyodysenteriae

Abstract: Nineteen of 22 female mice (CF1 strain) inoculated intragastrically with Treponema hyodysenteriae developed cecal and colonic lesions consisting of catarrhal inflammation, edema, and occasional hemorrhage.

Cited by 46 publications

References 5 publications

Phenotypic and molecular characterization of Brachyspira spp. isolated from wild rodents

Phenotypic and molecular characterization of Brachyspira spp. isolated from wild rodents

Cloning and characterization of Treponema hyodysenteriae antigens and protection in a CF-1 mouse model by immunization with a cloned endoflagellar antigen

Inactivation of aSerpula (Treponema) hyodysenteriaehemolysin gene by homologous recombination: Importance of this hemolysin in pathogenesis ofS. hyodysenteriaein mice

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