Experimental infection and vector competence of Amblyomma patinoi, a member of the Amblyomma cajennense species complex, for the human pathogen Rickettsia rickettsii

Martínez-Diaz, Heidy-C; Forero-Becerra, Elkin; Hidalgo, Marylin; Labruna, Marcelo Bahia

doi:10.1016/j.ttbdis.2021.101751

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…Vector competence of the Colombian tick species has only been described using R. rickettsii, which can be used to understand the transmission dynamics of this bacterial group. A. patinoi is considered the primary R. rickettsii vector [ 28 , 39 ], and this species has demonstrated a successful maintenance of R. rickettsii through transstadial perpetuation, transovarial transmission, and subsequent pathogen transmission post-colony passage perpetuation [ 83 ]. Despite this evidence, some authors argue that this vector is less susceptible to R. rickettsii infection compared to other vectors present in Colombia, which is likely due to the higher presence of non-pathogenic species among this vector [ 84 ].…”

Section: Vector Competencementioning

confidence: 99%

Spotted Fever Group Rickettsia spp. Molecular and Serological Evidence among Colombian Vectors and Animal Hosts: A Historical Review

Gual-Gonzalez,

Torres,

Self

et al. 2024

Insects

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Spotted fever group Rickettsia spp. (SFGR) are a large group of tick-borne bacteria causing important emerging and re-emerging diseases that affect animals and humans. While SFGR are found worldwide, a lack of surveillance and misdiagnosis particularly affect South American countries. Colombia is a high burdened country in South America, yet rickettsioses are not deemed a nationally reportable condition limiting disease-specific public health resources. As mortality rates are high for one Rickettsia pathogen species, there is a great need to better understand the epidemiological and ecological factors that increase SFGR transmission risk regionally. This literature review provides an overview of Colombia-based SFGR studies connecting knowledge about both vectors and hosts.

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Section: Vector Competencementioning

confidence: 99%

Spotted Fever Group Rickettsia spp. Molecular and Serological Evidence among Colombian Vectors and Animal Hosts: A Historical Review

Gual-Gonzalez,

Torres,

Self

et al. 2024

Insects

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“…Rickettsia rickettsii is responsible for Rocky Mountain spotted fever (RMSF) and the most important vectors are Ixodid ticks, including Rhipicephalus sanguineus (tropical lineage, now classified as R. linnaei ) , Dermacentor andersoni, D. variabilis, Amblyomma sculptum, A. americanum , and A. patinoi (Pinter et al, 2011 ; Piranda et al, 2011 ; Dergousoff et al, 2013 ; Parola et al, 2013 ; Levin et al, 2017 ; Costa et al, 2020 ; Martínez-Diaz et al, 2021 ; Šlapeta et al, 2022 ; Sánchez Pérez et al, 2023 ). RMSF occurs after the bite of an infected tick, with an incubation period of 7 days.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a novel detection method for Rickettsia rickettsii using a loop-mediated isothermal amplification assay

Carvajal-Gamez,

Olguín-Barrera,

Tinoco-Gracia

et al. 2024

Front. Microbiol.

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IntroductionRickettsia rickettsii is an obligate, intracellular pathogen and the causative agent of Rocky Mountain spotted fever (RMSF). RMSF is an important zoonotic disease due to its high fatal outcome in humans. The difficulty of clinical diagnosis due to the low sensitivity and specificity of current diagnostic methods are a principal setback. We reported the development of a new method for the detection of R. rickettsii in human and tick DNA samples using loop-mediated isothermal amplification (LAMP), as well as the validation of the LAMP test for R. rickettsii in field samples of infected ticks and humans, determining the diagnostic sensitivity and specificity, as well as the reproducibility of the test.MethodsThis technique uses hydroxy naphthol blue (HNB) as an indicator of the formation of magnesium pyrophosphate, a marker for the presence of DNA. Here, we used a putative R. rickettsii gene as a target for three pairs of primers that specifically amplify R. rickettsii DNA by hairpin-based isothermal amplification technique (LAMP).Results and discussionThe sensitivity of the assay was ~1.6–3 pg, which is 10 times more sensitive than PCR. To determine the diagnostics specificity and sensitivity, 103 human DNA samples and 30 tick DNA samples were evaluated. For the human samples, a sensitivity for HNB of 93%, a specificity of 70% and a k of 0.53 were obtained. For electrophoresis the sensitivity was 97% with a specificity of 58% and a k of 0.42. For tick samples, a sensitivity of 80% was obtained, a specificity of 93% for HNB and for electrophoresis the sensitivity and specificity were 87%. The k for both was 0.73. The degree of concordance between HNB and electrophoresis was 0.82 for humans and for ticks, it was 0.87. The result is obtained in shorter time, compared to a PCR protocol, and is visually interpreted by the color change. Therefore, this method could be a reliable tool for the early diagnosis of rickettsiosis.

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“…complex and it is considered the main vector of R. rickettsii in some areas of Central and South America (Guedes et al, 2005;Faccini-Martínez et al, 2015). However, a study found that A. patinoi might not be able to sustain R. rickettsii infection by transovarial transmission for successive tick generations without the horizontal transmission among animal hosts, and that this tick species has a low R. rickettsii infection rate under natural conditions (Martínez-Diaz et al, 2021). In Colombia, R. rickettsii has been detected from A. patinoi in an endemic area for Rocky Mountain Spotted Fever (RMSF) (Faccini-Martínez et al, 2015), thus its importance cannot be underestimated.…”

Section: Discussionmentioning

confidence: 99%

Estudio de la rickettsiosis transmitida por garrapatas en zonas rurales del departamento del Cauca y la importancia de Amblyomma patinoi en la epidemiología de la enfermedad

Díaz¹

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Some hard ticks' species can act as vectors of a wide variety of pathogens of human and animal importance such as Anaplasma, Ehrlichia and Rickettsia spp. In Colombia, a total of forty-six tick species have been described, and some of them have been implicated as vectors of some infectious agents. The department of Cauca is one of the thirty-two departments of Colombia. Most of its population lives in rural areas and depends on agriculture as the main economic activity, favoring exposure to ticks and tick-borne pathogens. Thus, the present study aimed to determine the tick species and tick-borne pathogens circulating in this region. From August to November 2017, ticks were collected from dogs, horses and cattle from eight rural areas of four municipalities in the department of Cauca. All collected ticks were classified according to taxonomic keys and organized in pools. DNA was extracted from all tick pools for molecular confirmation of tick species and detection of Anaplasma, Ehrlichia and Rickettsia spp. A total of 2809 ticks were collected which were grouped in 602 pools. Ticks were morphologically identified as Amblyomma cajennense sensu lato, Dermacentor nitens, Rhipicephalus microplus and Rhipicephalus sanguineus sensu lato. The molecular identity of A. cajennense s.l. was confirmed as Amblyomma patinoi. A total of 95% of the pools scored positive for members of the Anaplasmataceae family, of which, 7.8% and 7.3% were positive to Anaplasma and Ehrlichia spp., respectively, being identified as Anaplasma marginale, Ehrlichia minasensis and Ehrlichia canis; and 16.1% were positive for Rickettsia spp. with high identity for Rickettsia asembonensis, Rickettsia felis and Candidatus Rickettsia senegalensis. This is the first report describing the natural infection of ticks with rickettsial pathogens and the occurrence of A. patinoi ticks in Cauca department, Colombia.

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Experimental infection and vector competence of Amblyomma patinoi, a member of the Amblyomma cajennense species complex, for the human pathogen Rickettsia rickettsii

Cited by 4 publications

References 37 publications

Spotted Fever Group Rickettsia spp. Molecular and Serological Evidence among Colombian Vectors and Animal Hosts: A Historical Review

Spotted Fever Group Rickettsia spp. Molecular and Serological Evidence among Colombian Vectors and Animal Hosts: A Historical Review

Development and validation of a novel detection method for Rickettsia rickettsii using a loop-mediated isothermal amplification assay

Estudio de la rickettsiosis transmitida por garrapatas en zonas rurales del departamento del Cauca y la importancia de Amblyomma patinoi en la epidemiología de la enfermedad

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