Experimental Induction of Pulmonary Fibrosis in Horses with the Gammaherpesvirus Equine Herpesvirus 5

Williams, Kurt J.; Robinson, N. E.; Lim, Ailam; Brandenberger, Christina; Maes, Roger K.; Behan, Ashley; Bolin, Steven R.

doi:10.1371/journal.pone.0077754

Cited by 60 publications

(77 citation statements)

References 57 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study on the detection of EHV-5 in horses with EMPF and other respiratory diseases, the clinical signs observed in different groups were similar (Pusterla et al 2015), thus demonstrating their non-specificity. EMPF is difficult to diagnose in a living animal and may be suspected based on clinical and radiological examination, lung biopsy, and identification of EHV-5 by tracheal lavage with PCR analysis (Williams et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Equine multinodular pulmonary fibrosis in southern Brazil: pathology and differential diagnosis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Equine multinodular pulmonary fibrosis (EMPF) diagnosed at the Laboratório Regional de Diagnóstico of Faculdade de Medicina Veterinária, Universidade Federal de Pelotas (LRD/UFPel), is described. Differential aspects of other pulmonary diseases in horses with pneumonia and interstitial fibrosis were discussed. The disease occurred in a 15-year-old equine that presented with clinical signs of respiratory distress, intermittent fever, anorexia, and dyspnea. Macroscopically, there was enlargement of the lungs with whitish, pale, firm and well-delimited nodules, approximately 7-10 cm in diameter, distributed throughout the parenchyma. Histologically, the lung nodules had alveolar spaces with walls covered by cuboidal epithelium containing macrophages, neutrophils, lymphocytes, hyperplasia of type II pneumocytes and, eventually, multinucleated giant cells. The interstitium was markedly thickened by mature fibrous connective tissue and collagen. There were intranuclear inclusion bodies in the macrophages. The PCR technique for detecting the EHV-5 DNA was positive. In a retrospective study of pneumonia cases in horses with interstitial fibrosis diagnosed in the LRD/UFPel, two animals had macroscopic and histological lesions similar to those with EMPF, but they were negative for EHV-5 in PCR. Four cases diagnosed with pneumonia and interstitial tissue fibrosis had a histological pattern that was different from that observed in the EMPF animal, thus eliminating the possibility of EMPF. It is concluded that EMPF is a sporadic disease that should be considered in cases of respiratory disease in horses. Reports of such cases are important to alert technicians about the occurrence of rare diseases in Brazil. It is also necessary to establish the true role of EHV-5 in the pathogenesis of EMPF. Cases of pulmonary fibrosis such as EMPF, in which the virus is not present, should be studied to establish whether it could be an idiopathic form of the disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Equine multinodular pulmonary fibrosis in southern Brazil: pathology and differential diagnosis

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although such diseases have been recognized for 20 years in horses, they have only more recently been described in dogs and cats (6,7,(9)(10)(11)(12). Although such diseases have been recognized for 20 years in horses, they have only more recently been described in dogs and cats (6,7,(9)(10)(11)(12).…”

Section: Workhop Discussionmentioning

confidence: 99%

An Official American Thoracic Society Workshop Report: Comparative Pathobiology of Fibrosing Lung Disorders in Humans and Domestic Animals

Roman¹,

Brown²,

Olson³

et al. 2013

Annals ATS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The following selected references of the EHV-5 gB gene were used in the phylogenetic analysis: GenBank accession numbers AF050671 (Holloway et al, 1999), GQ325592 andGQ325593 (Thorsteinsdó ttir et al, 2010), KC715730, KC715731 and KC715732 (Williams et al, 2013), and NC_026421 (Wilkie et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…The strain obtained from the horse diagnosed with EMPF was classified as genotype III, with only 1 nt divergence from the reference nucleotide sequence (GenBank accession number KC715730) obtained from an EMPF case in the USA (Williams et al, 2013) (Figs 1 and 2). However, other healthy horses were also infected with strains of the same genotype.…”

Section: Clinical Linkmentioning

confidence: 99%

Genetic variation and dynamics of infections of equid herpesvirus 5 in individual horses

Back

Ullman

Leijon

et al. 2016

Journal of General Virology

View full text Add to dashboard Cite

Equid herpesvirus 5 (EHV-5) is related to the human Epstein-Barr virus (human herpesvirus 4) and has frequently been observed in equine populations worldwide. EHV-5 was previously assumed to be low to non-pathogenic; however, studies have also related the virus to the severe lung disease equine multinodular pulmonary fibrosis (EMPF). Genetic information of EHV-5 is scanty: the whole genome was recently described and only limited nucleotide sequences are available. In this study, samples were taken twice 1 year apart from eight healthy horses at the same professional training yard and samples from a ninth horse that was diagnosed with EMPF with samples taken pre-and post-mortem to analyse partial glycoprotein B (gB) gene of EHV-5 by using next-generation sequencing. The analysis resulted in 27 partial gB gene sequences, 11 unique sequence types and five amino acid sequences. These sequences could be classified within four genotypes (I-IV) of the EHV-5 gB gene based on the degree of similarity of the nucleotide and amino acid sequences, and in this work horses were shown to be identified with up to three different genotypes simultaneously. The observations showed a range of interactions between EHV-5 and the host over time, where the same virus persists in some horses, whereas others have a more dynamic infection pattern including strains from different genotypes. This study provides insight into the genetic variation and dynamics of EHV-5, and highlights that further work is needed to understand the EHV-5 interaction with its host.

show abstract

Experimental Induction of Pulmonary Fibrosis in Horses with the Gammaherpesvirus Equine Herpesvirus 5

Cited by 60 publications

References 57 publications

Equine multinodular pulmonary fibrosis in southern Brazil: pathology and differential diagnosis

Equine multinodular pulmonary fibrosis in southern Brazil: pathology and differential diagnosis

An Official American Thoracic Society Workshop Report: Comparative Pathobiology of Fibrosing Lung Disorders in Humans and Domestic Animals

Genetic variation and dynamics of infections of equid herpesvirus 5 in individual horses

Contact Info

Product

Resources

About