Experimental immunology The effect of pea albumins on immune response in mice

Złotkowska, Dagmara; Wasilewska, Ewa; Kubicka, Ewa; Wróblewska, Barbara

doi:10.5114/ceji.2012.30799

Cited by 4 publications

(5 citation statements)

References 16 publications

(20 reference statements)

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“…The cells were collected from the interface, washed, and phenotyped. Splenic, MLN, and PP lymphocytes were isolated using the conventional method (Holmes et al, 2001;Zlotkowska et al, 2012). Briefly, each tissue was homogenized by the Dounce homogenizer, passed through a Nitex filter (80-μm nylon mesh; Small Parts Inc., Miami, FL) to remove debris, and washed in IM medium [RPMI 1640 medium (Sigma-Aldrich) supplemented with 1 mM sodium pyruvate, 1 mM NEAA, and 10 U/ mL penicillin-streptomycin].…”

Section: Lymphocyte Isolation and Flow Cytometry Analysismentioning

confidence: 99%

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Wasilewska

Złotkowska

Wróblewska

2019

Journal of Dairy Science

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We investigated the yogurt starter cultures of Lactobacillus bulgaricus 151 and Streptococcus thermophilus MK-10 for their effect on the severity of experimental colitis, lymphocyte profile, and regulatory T-cell response. Colitis was induced in BALB/c mice via the administration of 3.5% dextran sulfate sodium salt (DSS) in drinking water for 6 d. Next, the mice were gavaged intragastrically with an active yogurt cultures (YC) mixture (~5 × 10 9 cfu/mouse per day) or saline (vehicle) for 8 d. Mice receiving DSS or saline alone served as positive and negative controls, respectively. The length of the colon, disease activity index, histological scores, myeloperoxidase activity, epitheliumassociated microbes, short-chain fatty acid profile, total IgA antibody-forming cells, CD3 + CD8 + , CD3 + CD4 + , CD3 + CD4 + CD25 + , CD3 + CD4 + CD25 + Foxp3 + T-cell subsets, and cytokine profiles (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and tumor necrosis factor) were examined after termination of the mice. Feeding mice with YC mixture reduced disease symptoms and modified intestinal microbiota and host inflammatory responsiveness to DSS. We observed limited weight loss and a decreased disease activity index score, lowered myeloperoxidase activity, and somewhat reduced damage of the intestine. The YC mixture upregulated the colon length, increased the amount and diversity of mucosaassociated microbes (enterobacteria, enterococci, and yeast), and decreased the concentration of putrefactive short-chain fatty acids in the cecal contents. It downregulated the input of cytotoxic CD3 + CD8 + T cells and CD3 + CD4 + CD25 + FoxP3 + regulatory T cells in Peyer's patches and enhanced CD3 + CD4 + CD25 + T cells in spleens and CD3 + CD4 + CD25 + FoxP3 + cells in peripheral blood mononuclear cells. Simultaneously, IgA antibody-forming cells were downregulated in mesenteric lymph nodes (MLN) and enhanced in spleens (SPL). The cultures mostly enhanced the production of cytokines tested in MLN and SPL, except for IL-6, which was downregulated in MLN. Interleukin-2 and IL-4 were the most upregulated in MLN, whereas IL-10, IL-4, IL-2, IFN-γ, and tumor necrosis factor were most upregulated in SPL. In serum, the YC mixture downregulated IFN-γ and clearly increased IL-2. Based on these results, we recognize the high anti-inflammatory and immunomodulatory potential of the L. bulgaricus 151 and S. thermophilus MK-10 set. The strains possess the ability to modulate the intestinal mucosal and systemic immune system toward both IgA production and induction of regulatory T cells, shifting Th1/Th2 balance.

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Section: Lymphocyte Isolation and Flow Cytometry Analysismentioning

confidence: 99%

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Wasilewska

Złotkowska

Wróblewska

2019

Journal of Dairy Science

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“…After the first contact with the antigen, naïve CD4 + T cells may differentiate into diverse subpopulations, the most important being Th1, Th2, Th17, and induced regulatory cells [ 28 ]. Our previous results showed changes in induction of CD4 + and CD8 + cells after different routes of immunization, suggesting that a percentage of these populations could serve as a marker for screening differences between immunity to different antigens [ 29 ]. We found κ-CN as an antigen that effectively induces ex-vivo lymphocyte proliferation.…”

Section: Resultsmentioning

confidence: 99%

Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies

et al. 2021

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The presence of various proteins, including modified ones, in food which exhibit diverse immunogenic and sensitizing properties increases the difficulty of predicting host immune responses. Still, there is a lack of sufficiently reliable and comparable data and research models describing allergens in dietary matrices. The aim of the study was to estimate the immunomodulatory effects of β-lactoglobulin (β-lg) in comparison to those elicited by κ-casein (κ-CN), in vivo and ex vivo, using naïve splenocytes and a mouse sensitization model. Our results revealed that the humoral and cellular responses triggered by β-lg and κ-CN were of diverse magnitudes and showed different dynamics in the induction of control mechanisms. β-Lg turned out to be more immunogenic and induced a more dominant Th1 response than κ-CN, which triggered a significantly higher IgE response. For both proteins, CD4+ lymphocyte profiles correlated with CD4+CD25+ and CD4+CD25+Foxp3+ T cells induction and interleukin 10 secretion, but β-lg induced more CD4+CD25+Foxp3- Tregs. Moreover, ex vivo studies showed the risk of interaction of immune responses to different milk proteins, which may exacerbate allergy, especially the one caused by β-lg. In conclusion, the applied model of in vivo and ex vivo exposure to β-lg and κ-CN showed significant differences in immunoreactivity of the tested proteins (κ-CN demonstrated stronger allergenic potential than β-lg), and may be useful for the estimation of allergenic potential of various food proteins, including those modified in technological processes.

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“…Then, cells were fixed in 250 μL of 2% PFA and analyzed using a BD LSR Fortessa Cell Analyzer (BD Biosciences, San Jose, CA, USA) equipped with DIVA software (BD Bioscience, San Jose, CA, USA. A total of 50 000 events were collected for each sample, and FlowJo software (FlowJo, LLC, Ashland, OR, USA) was used for subsequent data analysis . After cytometric analysis, the percentages of CD4 + and CD8 + T cells were recalculated for cell numbers, multiplying the flow cytometry results by the total cell numbers.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 50 000 events were collected for each sample, and FlowJo software (FlowJo, LLC, Ashland, OR, USA) was used for subsequent data analysis. 30 After cytometric analysis, the percentages of CD4 + and CD8 + T cells were recalculated for cell numbers, multiplying the flow cytometry results by the total cell numbers.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice

Chudzik-Kozłowska

Wasilewska

Złotkowska

2020

J. Agric. Food Chem.

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Green pea (Pisum sativum) is a component of European cuisine; however, an estimated 0.8% of Europeans suffer from allergies to pea proteins. We examined the immunoreactive potential of pea albumins (PA) in BALB/c and C57BL/6 mice. Mice were orally gavaged with PA or glycated pea albumins (G-PA) for 10 consecutive days, in combination with an adjuvant. Both PA and G-PA increased PA-specific serum antibody titers to about 2 12 for anti-PA IgG, ∼2 7 for anti-PA IgA, and ∼2 7.8 for anti-PA IgA in fecal extracts (p < 0.001). On day 42 postexposure, the antibodies titers decreased and were greater in BALB/c compared to C57BL/6 mice (p < 0.05). Distribution of CD4 + and CD8 + T cells in lymphoid tissues presented strain-specific differences. PA was found to induce lymphocyte proliferation; however, G-PA did not. Both PA and G-PA changed CD4 + and CD8 + T cells percentages in some lymphoid tissues; however, this did not impact cytokines production by splenocyte cultures evidenced by the stimulation of Th1, Th2, and Th17 cells. The observed immunomodulatory properties of PA and G-PA and lack of a sign of allergic reaction render them suitable for supplements in personalized diets, but further research is needed to precisely understand this activity.

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Experimental immunology The effect of pea albumins on immune response in mice

Abstract: The aim of presented studies was to determine the effect of total pea albumins on mice immune system response. Oral and intraperitoneal (i.p.)

Cited by 4 publications

References 16 publications

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Yogurt starter cultures of Streptococcus thermophilus and Lactobacillus bulgaricus ameliorate symptoms and modulate the immune response in a mouse model of dextran sulfate sodium-induced colitis

Differences in Regulatory Mechanisms Induced by β-Lactoglobulin and κ-Casein in Cow’s Milk Allergy Mouse Model–In Vivo and Ex Vivo Studies

Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice

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