Experimental evaluation of photocrosslinkable chitosan as a biologic adhesive with surgical applications

Ono, Katsuaki; Ishihara, Masayuki; Ozeki, Yuichi; Deguchi, Hiroyuki; Sato, Mitsuharu; Saitō, Yoshio; Yura, Hirofumi; Sato, Masato; Kikuchi, Makoto; Kurita, Akira; Maehara, Tadaaki

doi:10.1067/msy.2001.117197

Cited by 108 publications

(107 citation statements)

References 24 publications

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“…It carries a positive charge, as the free amino groups of β-(1-4)-linked D-glucosamine are protonated at physiological pH. Chitosan is being extensively used as a potential biomaterial in several medical devices and health care products owing to its biodegradability and advantageous biological properties, including hemostatic activity (15,16), biodegradability (15,17), antibacterial activity (18,19) and its ability to serve as a wound dressing agent to accelerate wound healing (16,20,21). Given these advantages, the use of chitosan as a stimulant for platelet activation can be highly effective in PRP therapy.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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Abstract. Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that exhibits high platelet activation ability was investigated. A total of 13 chitosan samples with different molecular weight (Mw) and degree of deacetylation (DDA) were individually added to blood samples of rats and the amount of growth factors, albumin and fibrinogen in plasma was measured. To examine the influence of plasma activated by chitosan on the proliferation of fibroblasts and adipose tissue-derived stromal cells (ASCs), the plasma was added to the culture medium of human fibroblasts and adipose tissue-derived stromal cells. Chitosan with a DDA of >75% increased the release of platelet factor 4 into the plasma. The amount of growth factors released into the plasma and platelet activation varied depending on the Mw and DDA, while albumin and fibrinogen were hardly affected. The proliferation rate was highest when using plasma activated by chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da. These results suggested that the effectiveness of PRP therapy may be improved by using chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da.

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Section: Introductionmentioning

confidence: 99%

Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability

Hattori

Ishihara

2017

Experimental and Therapeutic Medicine

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“…The sealing capability of various surgical glues has been studied and used by many researchers and clinicians [20]- [22]. The threat of getting infection from agents of the fibrin adhesive limits the widespread usage of the surgical glue in surgery, especially in the United States.…”

Section: ) Surgical Gluementioning

confidence: 99%

Radioactive seed immobilization techniques for interstitial brachytherapy

et al. 2008

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Let us know how access to this document benefits you Methods: We first analyze the possible causes for seed movement, and propose three potential techniques for seed immobilization: (1) surgical glue, (2) laser coagulation and (3) diathermy coagulation. The feasibility of each method is explored. Experiments were carried out using fresh bovine livers to investigate the efficacy of seed immobilization using surgical glue. Results:Results have shown that the surgical glue can effectively immobilize the seeds.Evaluation of the radiation dose distribution revealed that the non-immobilized seed movement would change the planned isodose distribution considerably; while by using surgical glue method to immobilize the seeds, the changes were negligible.Conclusions: Prostate brachytherapy seed immobilization is necessary and three alternative mechanisms are promising for addressing this issue. Experiments for exploring the efficacy of the other two proposed methods are ongoing. Devices compatible with the brachytherapy procedure will be designed in future.

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“…Its UV sensitivity and consequent crosslinking was dependent on azide groups that were introduced to the chitosan molecules. In vivo tests were conducted to evaluate its efficacy as well as the organism response (Ono et al, 2001). The final results showed that, 30 days after the surgical procedure, the adhesive was still present at implantation site surrounded by fibrous tissue.…”

Section: Bioadhesivesmentioning

confidence: 99%