Experimental evaluation of new chitin–chitosan graft for duraplasty

Shitiz

International Wound Journal

2017

234

119

Chitin and chitosan are biopolymers with excellent bioactive properties, such as biodegradability, non-toxicity, biocompatibility, haemostatic activity and antimicrobial activity. A wide variety of biomedical applications for chitin and chitin derivatives have been reported, including wound-healing applications. They are reported to promote rapid dermal regeneration and accelerate wound healing. A number of dressing materials based on chitin and chitosan have been developed for the treatment of wounds. Chitin and chitosan with beneficial intrinsic properties and high potential for wound healing are attractive biopolymers for wound management. This review presents an overview of properties, biomedical applications and the role of these biopolymers in wound care.

Section: Chitin and Chitosan Wound Dressingsmentioning

confidence: 99%

Chitin and chitosan: biopolymers for wound management

Shitiz

International Wound Journal

2017

234

119

“…The chitin particles were dispersed by stirring within the volume of a viscose solution to form a homogeneous solution. The solution of chitin/chitosan was placed into Petri dishes and dried for 3 days at room temperature [12,13]. After the drying, materials had a visually smooth lower surface and a rough upper surface (Fig.…”

Section: Graft Preparationmentioning

confidence: 99%

“…A crossshaped incision was created in the dura mater. The fascia lata was placed in the dura mater and was sown up with atraumatic suture material [12].…”

Section: Design Of the Experiments And Surgerymentioning

confidence: 99%

Cerebrospinal fluid composition after duraplasty with different substitutes in early and late postoperative periods (an experimental study)

et al. 2019

Objective. To study cerebrospinal fluid (CSF) changes after the duraplasty with autologous fascia, collagen-based material and chitin-chitosan membrane in early and late postoperative periods.Materials and methods. Chitosan-based films were made out of 3 % solution of chitosan for the research. We used 200 kDa chitosan (deacetylation rate 80-90 %) to produce chitin-chitosan membrane by using solvent evaporation method. For enhancing mechanical properties and reducing the degradation, chitin particles were added to the chitosan solution. Chitosan and chitin ratio was 80/20. The chitin/chitosan solution in Petri dishes was being dried out during 3 days at room temperature.Cerebrospinal fluid composition has been studied after the duraplasty with autofascia, collagen-based material and innovative chitosan-based graft in early and late postoperative periods. The duraplasty was performed by applying these materials to 90 Chinchilla rabbits breed. Animals were divided into three groups: I group -duraplasty using the fascia lata autograft, II group -duraplasty with the collagen-based material, III group -duraplasty using the chitin-chitosan membrane. The animals in the II and III groups were divided into 2 subgroups: A -plasty without fixing the material, B -plasty with fixing the material using atraumatic suture. CSF composition was studied before and after the operation had been performed in 2 weeks, 2 and 6 months. Results.The results of our study demonstrated the increase in density and protein level, the decrease in рН and glucose level and the extreme increase of cells, mostly neutrophils after the use of fascia lata for dural closure. At the same time, there were no substantial changes after dural closure with artificial collagen-and chitosan-based materials, the CSF composition normalized in 2 months after operation.Conclusion. The use of autologous fascia for duraplasty leads to an acute response of the cerebrospinal fluid in the early postoperative period and to residual pleocytosis. The chitosan-based graft application was followed by no complications at 6 months after surgery and only slight CSF response in the early postoperative period. There wasn't any significant difference in CSF composition in chitosan-and the collagen-based material usage. Given the lack of changes in SCF tests between suture and no suture graft fixation except for a slight increase in erythrocyte number in the early postoperative period, the choice of material fixation method is entirely dependent on the clinical situation and does not affect the cerebrospinal fluid state.

“…[1][2][3][4] In such cases, it is essential to create a water-tight closure of the dura in order to avoid cerebrospinal fluid (CSF) leaks, brain herniation, infections, adhesions, and cortical scarring. [5][6][7] In cases where the dura mater cannot be repaired by primary closure, a dural graft can be used.…”

Section: Introductionmentioning

confidence: 99%

“…They should support tissue regeneration and resorb while allowing endogenous neodura to grow; should not induce immunologic or inflammatory reactions; should be strong enough to provide a water-tight closure without rupturing; should be easy to handle and suture; and should be cost-effective. 4,6,16 Biodegradable synthetic materials have many of these required features, and indeed, they are gaining popularity in many different medical applications. [18][19][20][21] Several such materials were tested as dura mater grafts.…”

Section: Introductionmentioning

confidence: 99%

Local Tolerance and Biodegradability of a Novel Artificial Dura Mater Graft Following Implantation Onto a Dural Defect in Rabbits

Ramot

Harnof

Klein³

et al. 2020

Toxicol Pathol

Dura mater defects are a common problem following neurosurgery. Dural grafts are used to repair these defects; among them are biodegradable polymeric synthetic grafts. ArtiFascia is a novel synthetic and fibrous Dural graft, composed of poly(l-lactic-co-caprolactone acid) (PLCL) and poly(d-lactic-co-caprolactone acid). In this study, the biodegradability and local tolerance of ArtiFascia was evaluated in rabbits and compared with a bovine collagen matrix as a reference control. ArtiFascia implantation resulted in the formation of neo-dura at the site of implantation and recovery of the dural damage and the calvaria bone above. The implanted graft was completely absorbed after 12 months and the remaining macrophages were morphologically consistent with the anti-inflammatory M2-like phenotype, which contributes to tissue healing and are not pro-inflammatory. The site of the drilled skull bone had a continuous smooth surface, without exuberant tissue or inflammation and a newly formed trabecular bone formation indicated the healing process of the bone. These results support the local tolerability and biodegradability of ArtiFascia when used as a dural graft in rabbits. This study suggests that PLCL-based grafts including ArtiFascia are safe and effective to repair Rabbit Dura.