Experimental detection of conformational transitions between forms of DNA: problems and prospects

Zubova, Elena A.; Strelnikov, Ivan A.

doi:10.1007/s12551-023-01143-9

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Cited by 3 publications

References 172 publications

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VII Congress of Russian Biophysicists—2023, Krasnodar, Russia

Anashkina,

Rubin,

Gudimchuk

et al. 2023

Biophys Rev

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VII Congress of Russian Biophysicists—2023, Krasnodar, Russia

Anashkina,

Rubin,

Gudimchuk

et al. 2023

Biophys Rev

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Theoretical Investigation of the structure and Raman scattering properties of Hoogsteen- and Watson-Crick-type Adenine-Thymine base pair

Xiao,

Zhang,

et al. 2025

Chemical Physics

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Properties of DNA in concentrated aqueous solutions of LiCl suggest transition to C-DNA

Mazur,

Maaloum,

Gladyshev

2024

Preprint

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C-DNA represents a canonical DNA form related to B-DNA. While C-DNA is known to exist in air-dried fibers, its occurrence in aqueous solutions remains subject to debate. In fibers, the transition from B- to C-DNA is promoted by reduced hydration in the presence of certain monovalent cations (most notably Li+), and this process is generally associated with an increase in the helical twist. To understand if the B-to-C transition can occur in liquid media in principle, we analyzed properties of several circular DNA substrates in aqueous solutions with varying concentrations of LiCl (0.1-8 M). To this end, we estimated changes in the helical twist directly from circular dichroism (CD) spectra of a large supercoiled plasmid and, in parallel, assayed conformational changes in DNA minicircles by atomic force microscopy (AFM). We found that the helical twist increased continuously over the entire range of tested LiCl concentrations, without being subject to saturation even at 8 M LiCl. The overall increase in the helical twist was compatible with the B-to-C transition occurring in solution at a concentration of LiCl of about 8 M, suggesting that C-DNA should be stable above this level.

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Experimental detection of conformational transitions between forms of DNA: problems and prospects

Cited by 3 publications

References 172 publications

VII Congress of Russian Biophysicists—2023, Krasnodar, Russia

VII Congress of Russian Biophysicists—2023, Krasnodar, Russia

Theoretical Investigation of the structure and Raman scattering properties of Hoogsteen- and Watson-Crick-type Adenine-Thymine base pair

Properties of DNA in concentrated aqueous solutions of LiCl suggest transition to C-DNA

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