Experimental composites containing quaternary ammonium methacrylates reduce demineralization at enamel-restoration margins after cariogenic challenge

Maia, A C; Mangabeira, Adrielle; Vieira, R P; Neves, Aline de Almeida; Lopes, Ricardo Tadeu; Pires, Thais Maria; Viana, Gil Mendes; Cabral, Lúcio Mendes; Cavalcante, Larissa Maria; Portela, Maristela Barbosa

doi:10.1016/j.dental.2019.05.021

Cited by 13 publications

(9 citation statements)

References 35 publications

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“…3 The researches has focused on the addition of this property to the restorative materials since the main reasons for the failure in composite resin restoration are the development of secondary caries due to the formation of biofilms in the gaps of the restoration margins. 19,20,21 Thus, all new material introduced should seek to evaluate the presence of such antimicrobial activity.…”

Section: Discussionmentioning

confidence: 99%

Thiourethane-functionalized fillers: biological properties and degradation resistance

et al. 2021

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This study determined the effect of thiourethanefunctionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutans colonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.

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Section: Discussionmentioning

confidence: 99%

Thiourethane-functionalized fillers: biological properties and degradation resistance

et al. 2021

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“…[17,[20][21][22] Therefore, in this study, a cavity preparation was performed to simulate the conditions of pits and fissures and the Knoop microhardness test was carried out to assess demineralization around the preparation margins after 48-hour biofilm formation. Knoop microhardness has been widely used for this purpose [18,23,24] and the S. mutans biofilm metabolic activity test, which can simulate oral conditions, has been used to replace cyclic pH. [22] Bovine teeth were used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Although both fluoridereleasing materials could prevent demineralization, the RI group showed better results as it interfered with the demineralization process at the tooth/material interface compared to the other material tested, and was therefore compatible with the performance of ionomeric materials described in other studies. [18,23] These results can be explained by the quick effect of fluoridereleasing ionomeric materials within the first hours, acting initially at the tooth/material interface and remaining constant at lower concentrations. [30] In addition, prevention of demineralization occurred after aging of the material.…”

Section: Discussionmentioning

confidence: 99%

Effect of Pit and Fissure Sealants on the Prevention of Enamel Demineralization After Exposure to Streptococcus mutans Biofilm: An Vitro Study

Silva¹,

Portela²,

Pereira³

et al. 2020

J Pediatr Dent

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Objective: The aim of this study was to evaluate the effect of sealants on the prevention of enamel demineralization and on biofilm metabolic activity. Materials and Methods: Cavity preparations were performed on 45 blocks of bovine teeth (4x4x4 mm) randomly assigned to three groups (n=15): RI-Riva Light Cure®/SDI; EM-Embrace™ WetBond™ Pulpdent Corp.®; and CO-Natural Flow/DFL resin (negative control). The sealed blocks were subjected to thermocycling (500 cycles/30 s). Half of the blocks were covered with acidresistant varnish to create a control area (RI, EM, and CO) and an experimental area (RI-EX, EM-EX, and CO-EX). They were subsequently exposed to Streptococcus mutans biofilm for assessment of demineralization by the Knoop microhardness test (50 g/15 s), at 25, 50, 100, 150, and 200 μm from the interface. In another test, the cylinders of the sealants (4 mmx3 mm) were subjected to the biofilm metabolic activity test. Results: The RI-EX group showed higher microhardness than CO-EX (p<0.05) and less demineralization when compared to RI at all distances. Microbial activity was lower in EM compared to CO, but not statistically significant in relation to RI. Conclusion: Both sealants can inhibit enamel demineralization in the presence of S. mutans biofilm.

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“…Micro-CT analysis resulted helpful to evaluate the validation of temporary and innovative restoration solutions [67]. The mineralization of carious enamel for the identification of a regenerative treatment solution for dental caries [68], as well as the demineralization of tissues, as the enamel adjacent to restoration margins in cavities filled with experimental composites, from extracted bovine incisors [69], are some of the aspects that were investigated. Furthermore, there were assessed:…”

Section: Dental Treatments and Mineral Tissuesmentioning

confidence: 99%

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

Campioni¹,

Pecci

Bedini

2020

Applied Sciences

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Micro-computed tomography (micro-CT) is a consolidated imaging technology allowing non-destructive three-dimensional (3D) qualitative and quantitative analysis by the observation of microstructures with high resolution. This paper aims at delivering a structured overview of literature about studies performed using micro-CT in dentistry and maxillofacial surgery (MFS) by analyzing the entire set of articles to portray the state of the art of the last ten years of scientific publications on the topic. It draws the scenario focusing on biomaterials, in vitro and in/ex vivo applications, bone structure analysis, and tissue engineering. It confirms the relevance of the micro-CT analysis for traditional research applications and mainly in dentistry with respect to MFS. Possible developments are discussed in relation to the use of the micro-CT combined with other, traditional, and not, techniques and technologies, as the elaboration of 3D models based on micro-CT images and emerging numerical methods. Micro-CT results contribute effectively with whose ones obtained from other techniques in an integrated multimethod approach and for multidisciplinary studies, opening new possibilities and potential opportunities for the next decades of developments.

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Experimental composites containing quaternary ammonium methacrylates reduce demineralization at enamel-restoration margins after cariogenic challenge

Cited by 13 publications

References 35 publications

Thiourethane-functionalized fillers: biological properties and degradation resistance

Thiourethane-functionalized fillers: biological properties and degradation resistance

Effect of Pit and Fissure Sealants on the Prevention of Enamel Demineralization After Exposure to Streptococcus mutans Biofilm: An Vitro Study

Ten Years of Micro-CT in Dentistry and Maxillofacial Surgery: A Literature Overview

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