Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

Atif, Abdul-Raouf; Cis, Uǵis La; Engqvist, Håkan; Bagheri, Shervin; Mestres, Gemma

doi:10.1021/acsomega.1c05540

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Cells can recognize specific peptide domains in this protein, further regulate their fate, and ultimately affect the biological properties of the scaffolds ( Lutolf and Hubbell, 2005 ). Therefore, increasing protein surface coverage can improve cell adhesion and diffusion ( Atif et al, 2022 ). The structure of the scaffold affects the protein adsorption level.…”

Section: Mechanism Of Scaffold Structure Promoting Osteogenesismentioning

confidence: 99%

Study on the influence of scaffold morphology and structure on osteogenic performance

Zhou

Xiong²,

Liu³

et al. 2023

Front. Bioeng. Biotechnol.

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The number of patients with bone defects caused by various bone diseases is increasing yearly in the aging population, and people are paying increasing attention to bone tissue engineering research. Currently, the application of bone tissue engineering mainly focuses on promoting fracture healing by carrying cytokines. However, cytokines implanted into the body easily cause an immune response, and the cost is high; therefore, the clinical treatment effect is not outstanding. In recent years, some scholars have proposed the concept of tissue-induced biomaterials that can induce bone regeneration through a scaffold structure without adding cytokines. By optimizing the scaffold structure, the performance of tissue-engineered bone scaffolds is improved and the osteogenesis effect is promoted, which provides ideas for the design and improvement of tissue-engineered bones in the future. In this study, the current understanding of the bone tissue structure is summarized through the discussion of current bone tissue engineering, and the current research on micro-nano bionic structure scaffolds and their osteogenesis mechanism is analyzed and discussed.

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Section: Mechanism Of Scaffold Structure Promoting Osteogenesismentioning

confidence: 99%

Study on the influence of scaffold morphology and structure on osteogenic performance

Zhou

Xiong²,

Liu³

et al. 2023

Front. Bioeng. Biotechnol.

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“…30 Regards the adsorption mechanisms, Canpolat and Tatlisoz 34 presented a model for protein adsorption within a charged silica nanoparticle with an electrical double layer using finite element analysis. Atif et al 35 used a Langmuir isotherm-based model to characterize protein and cell adhesion on a biomimetic hydroxyapatite surface. The equations were discretized in the time dimension and solved numerically using finite element method.…”

Section: Introductionmentioning

confidence: 99%

Theoretical modeling approach for adsorption of fibronectin on the nanotopographical implants

Gao,

Zhao,

Wang

et al. 2023

Proc Inst Mech Eng H

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The success of orthopedic implants depends on the sufficient integration between tissue and implant, which is influenced by the cellular responses to their microenvironment. The conformation of adsorbed extracellular matrix is crucial for cellular behavior instruction via manipulating the physiochemical features of materials. To investigate the electrostatic adsorption mechanism of fibronectin on nanotopographies, a theoretical model was established to determine surface charge density and Coulomb’s force of nanotopography – fibronectin interactions using a Laplace equation satisfying the boundary conditions. Surface charge density distribution of nanotopographies with multiple random fibronectin was simulated based on random number and Monte Carlo hypothesis. The surface charge density on the nanotopographies was compared to the experimental measurements, to verify the effectiveness of the theoretical model. The model was implemented to calculate the Coulomb force generated by nanotopographies to compare the fibronectin adsorption. This model has revealed the multiple random quantitative fibronectin electrostatic adsorption to the nanotopographies, which is beneficial for orthopedic implant surface design. Significance: The conformation and distribution of adsorbed extracellular matrix on biomedical implants are crucial for directing cellular behaviors. However, the Ti nanotopography-ECM interaction mechanism remains largely unknown. This is mostly because of the interactions that are driven by electrostatic force, and any experimental probe could interfere with the electric field between the charged protein and Ti surface. A theoretical model is hereby proposed to simulate the adsorption between nanotopographies and fibronectin. Random number and Monte Carlo hypothesis were applied for multiple random fibronectin simulation, and the Coulomb’s force between nanoconvex and nanoconcave structures was comparatively analyzed.

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Universal Biomaterial-on-Chip: a versatile platform for evaluating cellular responses on diverse biomaterial substrates

Atif,

Aramesh,

Carter

et al. 2024

J Mater Sci: Mater Med

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Microfluidics has emerged as a promising approach for assessing cellular behavior in vitro, providing more physiologically relevant cell culture environments with dynamic flow and shear stresses. This study introduces the Universal Biomaterial-on-Chip (UBoC) device, which enables the evaluation of cell response on diverse biomaterial substrates in a 3D-printed microfluidic device. The UBoC platform offers mechanical stimulation of the cells and monitoring of their response on diverse biomaterials, enabling qualitative and quantitative in vitro analysis both on- and off-chip. Cell adhesion and proliferation were assessed to evaluate the biocompatibility of materials with different physical properties, while mechanical stimulation was performed to investigate shear-dependent calcium signaling in pre-osteoblasts. Moreover, the applicability of the UBoC platform in creating more complex in vitro models by culturing multiple cell types was demonstrated, establishing a dynamic multicellular environment to investigate cellular interfaces and their significance in biological processes. Overall, the UBoC presents an adaptable tool for in vitro evaluation of cellular behavior, offering opportunities for studying various biomaterials and cell interactions in microfluidic environments. Graphical Abstract

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Experimental Characterization and Mathematical Modeling of the Adsorption of Proteins and Cells on Biomimetic Hydroxyapatite

Cited by 3 publications

References 42 publications

Study on the influence of scaffold morphology and structure on osteogenic performance

Study on the influence of scaffold morphology and structure on osteogenic performance

Theoretical modeling approach for adsorption of fibronectin on the nanotopographical implants

Universal Biomaterial-on-Chip: a versatile platform for evaluating cellular responses on diverse biomaterial substrates

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