Experimental Brucella abortus infection in pigs

Vaccine safety studies of Brucella abortus S19 and S19ΔvjbR in pregnant swine

“…None of the vaccinated animals shed any vaccine strains in vaginal secretions, suggesting that the possibility of cross-contamination to other animals residing in the same pastures might be limited. Interestingly, previous studies in swine infected with B. abortus Strain 544 demonstrated the presence of bacteria in vaginal swabs when animals were inoculated with a dose ranging from 5 × 10 8 to 5 × 10 11 CFU via the subcutaneous or intraconjunctival route [17] further indicating that the vaccine candidates are attenuated compared to B. abortus wild-type.…”

“…Despite the endemic nature of B. suis in the feral swine population and the imminent zoonotic threat, only a handful of studies have been performed throughout the years to develop and evaluate potential vaccine candidates for swine [15], [16], [17], [18], [19], [20]. Historically, vaccination has been recognized as the most straightforward and powerful way to control infectious diseases.…”

“…Decreased susceptibility to abortion secondary to S19 vaccination in gilts might be explained by a decreased capacity of species other than B. suis to establish a marked or persistent infection in pigs. Studies conducted by Stuart et al [17] demonstrated that inoculation with Brucella abortus wild-type Strain 544 can only establish a short-lived infection for up to 99 days post-inoculation. This is different than what is usually observed in cattle where vaccination of adult pregnant cattle with S19 induces abortions at an approximate rate of 22% when animals are inoculated with the standard dose of 5.8 × 10 9 CFU/ animal [21], [27].…”

Diagnostic performance of serological tests for swine brucellosis in the presence of false positive serological reactions

“…Thus, although unable to synthesize S-LPS, B115 accumulates O-PS internally (Cloeckaert et al, 1992). Indeed, this O-PS is a potential cause of specificity problems in the FPSR context (Dieste-Pérez et al, 2014) and, in fact, it was reported that injection of B115 brucellin causes a rise in agglutinating antibodies to this antigen in pigs experimentally infected with Brucella (Stuart et al, 1987). Although this potential problem has been recently solved by the use of genetically defined Brucella R mutants blocked in O-PS biosynthesis (Dieste-Pérez et al, 2014), skin testing requires two veterinary visits and is thus somewhat cumbersome and expensive.…”

The Brucella abortus S19 Δ vjbR Live Vaccine Candidate Is Safer than S19 and Confers Protection against Wild-Type Challenge in BALB/c Mice When Delivered in a Sustained-Release Vehicle

“…In animals, brucellosis is a major cause of abortions and infertility (3,19,28). In humans, infection can cause a serious debilitating disease manifested as undulant fever, endocarditis, arthritis, and osteomyelitis (23).…”